网络环境下数字图书馆的安全与防范措施

阐述了网络环境下影响数字图书馆安全的因素，提出了保护数字图书馆信息安全的防范措施。     

混合架构的开放式实验室管理系统的设计

利用计算机网络和数据库技术,本文采用基于B/S和C/S混合的体系架构,设计并实现一个数据集中处理和分布式处理相结合的,具有较高实用性的开放式实验室管理系统,以促进实验室建设和实验室管理水平的不断提高。     

内科病房控制乙型肝炎病毒传播的探讨

内科病房住院病人中有相当数量的乙肝病人或病毒携带，控制院内HBV传播十分重要。南京市鼓楼医院的有效预防方法，对临床预防工作有一定的指导意义。     

中药制剂抗IBDV效果的观察

选用金蓝、黄花两种中药注射液 ,在非免疫鸡胚中进行抗IBDV效果试验 ,黄花和金蓝注射液在 5 0 0倍稀释浓度下 ,均具有抗IBDV作用 ,接种病毒后混合组试验中鸡胚平均存活 15 4h ,177h(P <0 .0 5 ) ,抑制组试验中平均存活 62h ,68.7h(P >0 .0 5 ) ,预防组实验中鸡胚被孵出 ,病毒对照组则平均存活只有 4 5h。     

In vitro study on ribozyme against hepatitis C virus

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.     

基于行动导向开发高职《B/S结构程序开发》课程

以行动导向教学观为指导,从典型工作任务分析出发,确定了职业能力及职业素质目标,详细阐述了教学项目设计、实施、考核的课程设计流程,探讨了高职B/S结构程序开发课程的教学设计和教学实施。     

脊髓亚急性联合变性24例临床分析

目的探讨脊髓亚急性联合变性（SCD）患者的临床特点、诊断及预后。方法回顾性分析24例SCD患者的临床特点、辅助检查结果、治疗及预后。结果 21例血清维生素B12水平降低。17例行脊柱MRI检查,10例有异常信号,呈条带状等T1长T2异常信号。24例患者均给予维生素B12肌肉注射,14例治愈（58.3%）,6例明显好转（25.0%）,4例效果不明显（16.7%）。结论 SCD患者主要表现为脊髓后索、侧索受累,周围神经受累亦较常见。及时应用维生素B12治疗,症状可明显好转。本病为可治性疾病,预后与病程密切相关,应重视早期诊断、治疗。     

基于RUP的C/S和B/S混合模式构建学籍管理系统

在分析了高校学籍管理系统的特点和功能模块的基础上,针对C/S和B/S各自的优缺点,提出了基于RUP的C/S和B/S混合模式的学籍管理系统设计思想;讨论了学籍管理系统的总体结构和功能设计。     

恶意邮件跟踪系统(MET)设计

介绍了MaliciousEmailTrackingsystem(MET)的结构和功能。MET由服务器和客户机组成,能自动报告网上传播的恶意邮件行为的统计信息,帮助系统管理员检测恶意邮件的网络入口,抑制恶意软件(特别是自我复制病毒)的传播,把损失减少到最低限度。     

Enhancing cellular immune response to HBV M DNA vaccine in mice by codelivery of interleukin-18 recombinant

Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant.