图书情报学高被引论文引用现象的实证研究

学术论文是科研活动产出和知识信息交流的主要载体,而学术论文被引用则是学术成果获得认同的主要方式之一。研究高被引论文所引用论文的分布特征,对于了解高被引论文知识基础的构成具有重要意义。本文以ISI图书情报学（Information Science & Library Science）为例,以实证研究为基础,按比例采集图书情报学领域的高被引论文数据,查询这些高被引论文的参考文献的被引频次数据,以期得到高被引论文的引证特点。研究发现,图书情报学领域的高被引论文更多地引用了高被引参考文献;随着被引频次的降低,其引用的高被引论文所占的比例也在下降。     

图书情报核心期刊高被引论文统计分析与研究

文章以中国学术期刊网络出版总库为信息源,对19种图书情报核心期刊进行统计,以≥70被引频次的高被引论文501篇为研究样本,从被引文献的年代分布、被引频次、著者、机构、来源期刊、地区分布以及研究主题等方面进行分析研究,旨在揭示图书情报学高被引文献全面而详细的信息,为图书情报工作人员开展学术研究提供参考依据。     

教育理念的凝炼与个性化办学思想的生成

建设中国特色社会主义事业迫切需要造就一大批教育家型校长。而拥有独特的教育理念和个性化的办学思想是教育家型校长的重要特征。本文主要围绕中学校长＂凝炼教育理念和办学思想的必要性、理念的内涵、以及如何提炼教育理念和形成办学思想＂等方面进行了论述。     

荷电水雾脱硫实验研究

提出塔内荷电水雾脱硫塔外固硫的新型湿法脱硫工艺,重点对荷电水雾的脱硫特性进行了研究,结果表明,增加荷电电压与液／气比均有利于增加脱硫效率,但仅采用水雾作为吸收剂,总的吸收效率相对较低;液／气比低时增加荷电电压对脱硫效率的影响并不明显,随着液／气比增加,荷电电压对脱硫效率的影响有所增强;水雾荷电后能够提高SO2溶解速率,提高SO2吸收率,有利于降低湿法脱硫初期投资及运行成本．     

Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system

BackgroundThe heterologous expression of parasitic proteins is challenging because the sequence composition often differs significantly from host preferences. However, the production of such proteins is important because they are potential drug targets and can be screened for interactions with new lead compounds. Here we compared two expression systems for the production of an active recombinant aldehyde dehydrogenase (SmALDH_312) from Schistosoma mansoni, which causes the neglected tropical disease schistosomiasis.ResultsWe produced SmALDH_312 successfully in the bacterium Escherichia coli and in the baculovirus expression vector system (BEVS). Both versions of the recombinant protein were found to be active in vitro, but the BEVS-derived enzyme showed 3.7-fold higher specific activity and was selected for further characterization. We investigated the influence of Mg²⁺, Ca²⁺ and Mn²⁺, and found out that the specific activity of the enzyme increased 1.5-fold in the presence of 0.5 mM Mg²⁺. Finally, we characterized the kinetic properties of the enzyme using a design-of-experiment approach, revealing optimal activity at pH 7.6 and 41°C.ConclusionsAlthough, E. coli has many advantages, such as rapid expression, high yields and low costs, this system was outperformed by BEVS for the production of a schistosome ALDH. BEVS therefore provides an opportunity for the expression and subsequent evaluation of schistosome enzymes as drug targets.How to cite: Harnischfeger J, Beutler M, Salzig D, et al. Biochemical characterization of the recombinant schistosome tegumental protein SmALDH_312 produced in E. coli and baculovirus expression vector system. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.002     

一种3G手机网络游戏服务器架构的分层设计

3G手机网络游戏互动娱乐应用平台基于3G无线网络的高带宽及封装的底层协议、高性能服务器端、跨平台客户端等技术,以实现业内最有挑战性的MMORPG游戏类型为目标.该系统建成能有效提高数据通讯传输速率、保障数据安全、确保达成即时互动和保障用户体验.     

新时期开放大学在高技能人才培养中的作用及发展思路

本文首先分析了开放大学与高技能人才培养的必要性,以及开放大学在高技能人才培养中的作用。之后,文章提出了新时期开放大学在高技能人才培养中发展思路,主要包括：加快开放大学办学思路改革,提升高技能人才培养意识;发挥政府引导作用,构建开放大学与人才培养对接体系;不断完善开放大学办学机制,构建良好的人才培养氛围。本文旨在结合开放大学的特点,探索其在新时期高技能人才培养中的作用及发展思路,为更好挖掘开放大学的价值和促进我国高技能人才培养工作开展提供参考。     

New Charged Charmonium-like States Observed at BESIII

<正>Since the discovery of the electrically-charged Zc(3900),a possible four-quark object,the Beijingbased BESIII Collaboration has made a rapid string of related discoveries.While quarks have long been known to bind together in groups of twos or threes,these new results seem to be quickly opening the door to a previously elusive type of four-quark matter.The most recent breakthroughs by the BESIII Collaboration have come about through a dedicated study of the decays of the mysterious"Y(4260)"particle,which has a well-established mass that is inconsistent with the interpretation that it consists only of a charm quark and     

体育教学中语言的特点

只有在教学中准确、合理、灵活地运用体育教学语言,才能较好地完成教学任务,提高教学质量,促进学生的发展。     

补充肌酸对大强度运动能力的影响

根据肌酸的生物合成特点及其在能量代谢和疲劳中的作用,阐述了额外补充肌酸对运动效果的影响机理,并论述了大强度运动中补充肌酸的基本原理与方法.