Three-dimensional tracking of GLUT4 vesicles in TIRF microscopy

TIRF microscopy has provided a means to view mobile granules within 100 nm in size in two dimensions. However quantitative analysis of the position and motion of those granules requires an appropriate tracking method. In this paper, we present a new tracking algorithm combined with the unique features of TIRF. Firstly a fluorescence correction procedure was processed to solve the problem of fluorescence bleaching over time. Mobile granules were then segmented from a time-lapse image stack by an adaptive background subtraction method. Kalman filter was introduced to estimate and track the granules that allowed reducing searching range and hence greater reliability in tracking process. After the tracked granules were located in x-y plane, the z-position was indirectly inferred from the changes in their intensities. In the experiments the algorithm was applied in tracking GLUT4 vesicles in living adipose cells. The results indicate that the algorithm has achieved robust estimation and tracking of the vesicles in three dimensions.     

Switchable 2,3-dithienylmaleimide bonded to different fluorophores:synthesis and photochromic properties

A series of photochromic diarylethene derivatives containing different fluorophores was synthesized by the Sonogashira coupling reaction and characterized by ^1H nuclear magnetic resonance （NMR）, ^13C NMR and mass spectroscopy. Photochromic conversion of Cz-MS was observed and its structural change was confirmed by proton NMR, Switchable absorption and emission phenomena were observed for these molecules. These phenomena were attributed to the reversible photochromic fluorescence resonance energy transfer （pcFRET） process. The design of this novel photochromic system of Py-4MS enables information to be processed in a non-destructive manner. This overcomes the problem of the destructive nature of tracking photochromism using ultraviolet （UV）-vis spectroscopy.     

跨越时空的内在契合——关于杨朔、余秋雨散文之比较

杨朔与余秋雨散文是当代散文史上的两颗奇葩,尽管跨越了30年时空,但他们的散文在创作流变上经历了一个从创新的"范式"到僵化的"模式"的过程,二者的散文不仅有着对主流意识形态的迎合也有着内在精神上的契合.这样的相似之处是与他们所处的历史语境紧密关联的.     

测音诊病仪理论初探

元极学理论揭示了万物在运动过程中皆发出音，人体五脏运化发出五脏之音，五脏和谐运化则身体健康，据此，提出了开发测音诊病仪的设想，从理论基础，实践性，可行性等方面进行了论述，认为利用高新科技开发这种设备是可行的。     

上市公司内部审计模式及其选择

上市公司内部审计的重要性体现在,为上市公司履行守钶责任提供保障;可以协助上市公司完善风险管理,减小上市公司风险;可以协助上市公司完善治理结构;是促进管理,提高效益的推动力量。在选择上市公司内部审计模式时,应分别从公司性质、公司治理体系和公司管理水平上进行考虑。     

地方高校教师转型发展:逻辑、困境与突破路径

地方高校教师转型发展的内在逻辑包括三个方面:转变理念是地方高校教师转型发展的基础;优化队伍是地方高校教师转型发展的核心;政策推动是地方高校教师转型发展的保障。在转型发展过程中,地方高校教师面临着转型发展主体性意识不强、应用型动力不足、缺乏有效的机制和生态式管理等四重困境。基于此,对地方高校教师转型发展的突破路径进行探讨,有助于促进地方高校的转型发展。     

制度伦理及其限度

制度伦理适应了社会转型的需要，有效地解决了当前的道德危机。但制度伦理有其限度，即不是所有的伦理都能制度化；制度伦理有悖于道德生成论；制度伦理的价值指向只溯及到形式公正；制度伦理不是伦理建设的最高形式。     

企业财务风险的成因及对策

分析了企业财务风险的外部因素与内部因素;每个企业财务风险是不可避免的,但必须进行具体分析成因,以有效防范和化解财务风险。     

基于LabView的脉冲核磁共振实验

针对现有脉冲核磁共振实验用示波器显示和测量实验数据精度差的缺点,提出利用计算机声卡采集实验数据,用LabView软件开发实验数据采集及分析系统,具有测量精度高、操作简单、智能化分析的特点。     

核磁共振成像技术教学实验研究

为了克服现有核磁共振成像实验仪不能很好地满足直观实验教学需要之不足,同时为小样品小范围测试进行科学研究提供方便,并为教学单位提供价格相对低廉的产品.利用核磁共振成像的基本原理,采用NMI20型台式核磁共振成像仪,研究如何开展核磁共振成像技术教学实验问题.开发了涉及共振频率、脉冲序列、相位编码、加权成像、射频功放等内容的26个实验.作为教学实验设备,该成像仪备具有直观的界面以及成像过程的开放性,能使学生深入掌握核磁共振的原理、结构及其硬件系统,教学效果良好.