Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.     

Testing and ideology: policy debates about literacy assessments for Colorado’s bilingual students

ABSTRACT

The Colorado Reading to Ensure Academic Development Act requires grade-level attainment in literacy in English for students in grades K-3. Its practical outcome, however, has been to pressure schools with bilingual programs to shift their instructional language allocations towards more English in the early grades. Proposed rule revisions debated by the state Board of Education sought to facilitate testing in students’ language of instruction for those in bilingual programs. Analysis of written and verbal opposition to the proposed rule revisions demonstrates the persistence of insidious ethnoculturalist discourses opposing bilingual education as well as the cooptation of liberal multiculturalist discourses that, while framing bilingualism as an asset, argue that it is underdeveloped in bilingual programs that do not include English instruction from the outset. Given that bilingual instruction has demonstrated time and again that it benefits students acquiring English in schools, such new discursive turns pose a threat that must be recognized to fend off further legislative and regulatory attempts against bilingual education.     

雌激素受体(ER)与基因蛋白(P53、erbB-2)在胃癌中表达的研究

目的:探讨ER、erbB-2和P53在胃癌表达及相关性。方法:取胃癌手术标本119例,SP免疫组织化学染色。结果:ER、erbB-2和P53在胃癌表达率分别为63.87％,75.51％和73.11％;三者同时阳性者31.93％;ER与P53同时阳性者37.82％;ER与erbB-2同时阳性者33.61％;ER在高分化组阳性率高于低分化组(P<0.05);而erbB-2和P53在低分化组阳性率高于高分化组。70岁以上组P53、ER和erbB-2表达率均较高。结论:ER、P53和erbB-2在胃癌中表达率与癌瘤分化程度相关。P53和erbB-2有相关性,而且它们是胃癌预后不良的指标。     

2-（1，2-亚乙／1，3-亚丙二硫）亚甲基-3-羰基-N-芳（烷）基丁酰胺制备的新方法

以易制备的2-（1,2-亚乙／1,3-亚丙二硫）亚甲基-3-羰基丁酸1为原料,经与氯化亚砜的氯化反应后,与胺反应成功地以较好产率（40．5％-72．0％）合成重要的有机合成中间体2-（1,2-亚乙／1,3一亚丙二硫）亚甲基-3-羰基-N-芳（烷）基丁酰胺3．     

6-甲基-2-（α-羟甲基）-1H-苯并咪唑荧光增敏探针法测定蛋白质

以6-甲基-2-（α-羟甲基）-IH-苯并咪唑（MHMBM）作为荧光探针,利用蛋白质对MHMBM荧光增敏作用,建立了测定人血清总蛋白的新方法。结果表明,在中性水溶液中,MHMBM的荧光强度与牛蛋白（BSA）的浓度呈良好的线性关系,相关系数（r）为0．9995,检出限2．3μg／mL,线性范围4．6—60．0μg／mL。本方法用于测定两种样品溶液中的总蛋白,RSD（n=6）分别为1．7％和1．3％,回收率95．5％和98．6％。     

地方高校实验教学示范中心建设的研究与实践

高等学校实验教学示范中心将引领我国高校实验室建设和实验教学改革的发展趋势,国家级实验教学示范中心建设被列为教育部本科教学质量工程之一,作为地方高校如何创建省级和国家级实验教学示范中心,文中提出了多出精品,创出特色的建设思路。     

对青少年运动员胫骨疲劳性骨膜炎的实验研究

笔者针对青少年运动员中胫骨疲劳性骨膜炎发病率高的特点，自1999年以来，以来 往信函和个别访谈的方法，对河南、湖南、湖北、广东和广西五省35所高级中学、10所高等 院校体育系和8所体校的410例患者采用1/1000Hg2(NO3)2．2H2O（千分之一硝酸亚汞）进行 局部贴敷，并配合理疗、点穴按摩等方法，取得显著疗效，治愈率高达96%，深受青少年体 育爱好者的欢迎．     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients withHelicobacter pylori infection

Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) andHelicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status ofH. pylori was determined by histologic examination, RUT,¹⁴C-UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients withH. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml,P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection ofH. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml, respectively (P<0.05). The serum levels of sICAM-1, proved to be significantly correlated with the density ofH. pylori colonization in gastric mucosa (r _s=0.316,P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated thatH. pylori infection up-regulates the expression of sICAM-1.     

差分的应用及正整数的k次方幂求和

提出了利用差分表和二项式定理推导并求解前n个正整数的k次方幂之和S(n,k)=1k 2k nk的方法。