固定在脂质双层膜内的细胞色素c氧化酶电化学研究

含有单体牛细胞色素c氧化酶的脂质双层膜，被成功地固定在金石英晶体微平衡电极上．在较宽的pH值、温度和缓冲浓度范围内观察到了氧化酶修饰电极上的直接电子迁移．氧化酶修饰电极在80℃以上时保持直接电子迁移性质。将电极冷却到室温时，氧化酶仍保持电子迁移能力．在22～80℃范围内。温度变化可引起细胞色素c氧化酶的相转移，估计出了相转移前后的相应反应活化能通过射流分析，研究了在溶液含有亚铁细胞色素c氧化酶的电氧化过程中。乙腈同细胞色素c氧化酶的结合．结果显示：浓度1．3M具有常数Ki时。细胞色素c氧化酶和乙腈形成配合物．每个细胞色素c氧化酶配一个乙腈分子。乙腈同细胞色素氧化酶的结合过程是可逆过程．     

Effects of the crude extract of Polygala tenuifolia Willd on human sperm in vitro

The aim of the present study is to analyze sperm membrane changes and the spermicidal effect in treatment with the crude extract from Polygala tenuifolia Willd (PTW) in vitro. The root of PTW was extracted in distilled water. Normal human spermatozoa were used to assess the spermicidal activity (Sander-Cramer assay) of the extract from the PTW root. The hypo-osmotic swelling (HOS) test and the eosin Y (EY) staining were used to detect the integrity of sperm membrane and vitality. The sperm chromatin dispersion (SCD) test was performed to determine sperm DNA integrity. N-9 was used as a reference standard and semen added to physiological saline was used as the control. Semen samples were donated by 42 healthy fertile men. The crude extract from the root of PTW could immobilize and kill 100% spermatozoa within 20 s in vitro at the concentrations of 20.0 and 10.0 mg/ml; at the concentration of 5.0 mg/ml, spermatozoa were immobilized in (39.5±3.2) s. In the groups of the crude extract from the root of PTW and N-9 solution, the rate of the normal HOS (tails swollen) and the white head (unstained) was 0%, and the rate of the abnormal HOS (tails unswollen) and red head (stained) was 100%. Sperm DNA fragmentation showed no change in exposure to the crude extract from the root of PTW and N-9 solution. The sperm revival test did not show any spermatozoa that recovered their motilities. The rapid spermicidal activity of the crude extract from the root of PTW in vitro may occur by the disruption of the sperm membrane integrity.     

微孔分子箱的合成

利用连吡啶类配体和过渡金属离子进行自组装,合成了一类新的微孔分子箱。通过红外光谱,X-衍射以及热重分析对化合物结构和性质进行了研究。     

不同生境甜槠黄酮类化合物含量及成分分析

运用分光光度法测定了来自浙江台州境内天台山不同生境的甜槠黄酮类化合物的含量并对其成分进行了聚酰胺薄层层析分析。结果表明,甜槠不同生境的叶片黄酮类化合物以冠层生境含量最高,灌丛生境次之,林下生境含量最低;林下生境的黄酮类化合物成分最少,而冠层生境的成分最多。     

热休克蛋白90α在人胃癌与癌旁及淋巴结转移癌中表达的研究

目的 :研究热休克蛋白 90α(HSP90α)在人胃癌与癌旁及淋巴结转移癌中的表达。方法 :应用兔抗人HSP90α多克隆抗体SP免疫组化方法检测 93例胃癌、癌旁和淋巴结转移癌组织。结果 :HSP90α在胃癌、胃旁及淋巴结转移癌中的阳性率分别为 86.5 %、5 9.5 %、71.4 %。HSP90α在胃癌中的表达率高于癌旁 (P <0 .0 5 ) ,而与淋巴结转移癌差异不显著。结论 :HSP90α在人胃癌中表达值高于癌旁 ,此现象说明HSP90α和癌的产生和发展有密切联系。     

矩形膜受迫振动问题的数学模型

对于长为阿a,宽为b的矩形膜的受迫振动的问题,建立关于该问题的数学模型。利用分离变量法和二重傅里叶级数的方法,得到了矩形膜的受迫振动初边值问题的解。     

Amniotic membrane transplantation for porous sphere orbital implant exposure

Objective： This study is aimed at describing the clinical outcome of amniotic membrane transplantation for exposure of porous sphere implants. Methods： A retrospective review of consecutive cases of porous sphere orbital implant exposure was carried out. Eight cases were presented between May 2004 and Oct. 2006 （5 males, 3 females; mean age 44.5 years）. Six had enucleation and two had evisceration. Exposure occurred in two primary and six secondary. Orbital implant diameter was 22 mm in seven cases and 20 mm in one case. Six patients are with hydroxyapatite and two with high-density porous polyethylene （Medpor） orbital implants. The mean time from implantation to exposure was 1.1 months （range 0.8-2 months）. All patients required surgical intervention. Results： The time of follow-up ranged from 3.0 to 28.0 months （mean 16.5 months）. Amniotic membrane grafting successfully closed the defect without re-exposure in all of these patients. The grafts were left bare with a mean time to conjunctiva of about 1 month （range 0.8-1.5 months）. Conclusion： Exposed porous sphere implants were treated successfully with amniotic membrane graft in all of patients. The graft is easy to harvest. This technique is useful, dose not lead to prolonged socket inflammation and infection, and it is valuable application extensively.     

等离子体处理对聚四氟乙烯微孔薄膜界面粘结性能的影响

以XPS、动态接触角分析仪和扫描电镜等为手段,研究了不同条件下低温等离子体对聚四氟乙烯微孔薄膜界面粘结性能的影响。结果表明：等离子体处理功率对薄膜界面粘结性能的影响．因等离子体种类的不同而有明显的差别。等离子体处理时间对薄膜界面粘结性能的影响,因等离子体种类和处理功率的不同而有一定的差别。不同的等离子体,对薄膜界面粘结性能的改善效果,因等离子体处理功率的不同而有较明显的不同。     

膜萃取和流动注射在线样品前处理技术

对用膜萃取和流动注射技术进行样品的在线预处理方法进行了较为系统的研究。第一部分提出了一种新的样品前处理技术———连续流动液膜萃取(CFLME),并用于极性有机污染物的痕量富集。阐述了CFLME的基本原理,并以磺酰脲类除草剂和内分泌干扰物双酚 A为模型化合物,研究了CFLME的影响因素。在优化的条件下,甲磺隆和双酚 A分别经过 1 2 0min和 40min的萃取后,可达到 1 0 0 0倍和2 0 0倍的富集倍数,富集效率是SLM的 3.5～ 2 0 0倍。在此基础上,建立了CFLME 高效液相色谱(HPLC)在线联用测定磺酰脲类除草剂的方法,样品富集 1 0min就能达到 0.0 5～ 0.1 μg/L的检测限;建立的CFLME C1 8预柱 HPLC/紫外检测器在线联用系统,可测定地表水中ng/L级的磺酰脲类除草剂,其检测限比用C1 8SPE柱富集、HPLC/紫外检测器测定时低 2 0 0倍。研究表明,CFLME的主要优点是高选择性、高富集效率、低成本、液膜长期稳定,并易与各种分析仪器联用。第二部分研究了几种流动注射(FI)在线样品前处理技术,为FI应用于日常分析提供了新的思路和途径。建立了FI微孔膜液萃取(MM LLE)系统,用于洗涤剂中阴离子表面活性剂的日常测定;发展了FI高温反应系统,并成功地用于洗涤剂中的总无机磷酸盐以及烟草中总还原糖的自动分析;提出用试剂注入 FI技术     

一种重要的光合膜蛋白——菠菜主要捕光复合物的晶体结构

由生物物理研究所主持,植物研究所参加完成的"菠菜主要捕光复合物的晶体结构"研究成果以Article的方式发表在2004年3月18日出版的Nature上,该晶体结构图被选作本期杂志的封面图案.本文介绍了该项成果的研究背景、意义、主要创新点及方法.