Characterization of cellulose acetate micropore membrane immobilized acylase I

This paper describes an innovative method for the immobilization of acylase I, which was entrapped into the CA-CTA micropore membrane. The most suitable casting solutions proportion for immobilizing the enzyme was ob tained through orthogonal experiment. Properties of the enzyme membrane were investigated and compared with those of free enzyme and blank membrane. The thermal stability and pH stability of the enzyme inside the membrane were changed by immobilization. The optimum pH was found to be 6.0, which changes 1.0 unit compared with that of free acylase I. The optimum temperature was found to be about 90 ℃C, which is higher than that of free acylase I (60 ℃). Experimental results showed that immobilization had effects on the kinetic parameters of acylase I.     

Morphological characterization of Al_2O_3 membrane fabricated by sol-gel dip-coating method

1 Introduction Alumina membrane has received considerable atten-tion for its thermal ,chemical and mechanical stabilityin filtration applications .Alumina membraneis usuallyprepared by sol-gel process ,in which uniform nano-meter-sized particles can be pr…     

能量回收和节能过程中的膜技术

本文介绍了膜技术在能量回收和节能过程中的应用和发展动向。对膜技术在制能过程中的应用做了重点介绍,如以煤气化和液化来生产煤合成气制能过程以及由生物资源制能过程中的膜技术,涉及富氧膜及装置,电化学熔盐膜用于酸气的脱除等,并对渗透汽化膜技术代替蒸馏过程的节能意义进行了讨论。     

Determination of the genus-specific antigens in outer membrane proteins from the strains of<Emphasis Type="Italic">Leptospira interrogans</Emphasis> and<Emphasis Type="Italic">Leptospira biflexa</Emphasis> with different virulence

Objective: To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) ofleptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains ofLeptospira interrongans belonging to 15 serogroups and 2 strains ofLeptospira biflexa belonging to 2 serogroups. The outer envelopes (OEs) ofLinterrogans serogroupIcterohaemorrhagiae serovarlai strainlai (56601) with strong virulence and serogroupPomona serovarpomona strainLuo (56608) with low virulence, andL. biflexa serogroupSemaranga serovarpatoc strainPatoc I without virulence were prepared by using the method reported in Auranet al. (1972). OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen, respectively. Results: All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256–1:512. A similar SDS-PAGE pattern of the OMPs from the three strains ofleptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately 60 Kda. A positive protein fragment with approximately 32 KDa confirmed by Western blot, was able to react with the antiserum against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains ofleptospira. Conclusion: There are genus-specific antigens on the surface ofL.interrogans andL.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens ofleptospira. Project (No. 39970678) supported by the National Natural Science Foundation of China     

溶胶-凝胶浸渍法制备氧化铝膜的形貌表征

Alumina membranes without pinholes and cracks were prepared by the sol-gel process using anunordum aluminium sulphate as the starting material. The effects of different preparing conditions on morphology characteristics of the membrane were investigated by scanning electron microscopy and 3D rotational microscopy. The preparing conditions include the amounts of drying control chemical additives （DCCA）, sintering procedure and sol-gel concentration. The results showed that PVA is a good crack-preventing reagent and the morphology of supported membranes was affected by ninny factors, including Al2O3 concentration, PVA/Al2O3 ratio, heating rate, membrane thickness and intrinsic defects of the substrate surface.     

类脂双层的电学性质

类脂双层研究的动力来自动物世界,1961年首次报道了生物体的双层类脂膜(BLM)自组装.BLM体系已广泛应用于对生物能、光合成、免疫学和生物传感器等多种物理学现象的研究.文章在综述了类脂双层的起源及形成机理的基础上,从理论上分析了类脂双层的电学参量,以及类脂双层应用于生物传感器的应用前景.     

Modelling and control PEMFC using fuzzy neural networks

INTRODUCTION With worldwide increase of air pollution and the environmental consciousness of governments,people have to look for new resources to mitigate the energy crisis and improve the present environmental status(Baschuk and Li,2000;Rowe and Li,2001).Fuel cells are highly efficient and environmentally clean electricity generators(Berning et al.,2002)that convert the chemical energy of a gaseous fuel directly into electrical energy and play an important role in solving the energy pro…     

Comparative analysis of RBC membrane lipids in thalassemia, and iron deficiency anemia in relation to hypochromia and oxidant injury

The effect of an intrinsic defect in the red cell and pronounced hypochromia on oxidative damage to RBC membrane lipids was compared in beta-thalassemia and iron deficiency anemia (IDA), which have a varied etiology but equivalent low hemogiobin content. The study was planned to correlate the etiology of the disorders to the severity of lipid imbalance and RBC hemolysis in membranes of both the conditions. Results indicated a fall of lysophosphatidylcholine(LPC), phosphatidylethanolamine(PE) and the unsaturated to saturated fatty acid ratio in both conditions, while phosphatidylcholine(PC) increased only in thalassemia. However, irrespective of the disease, sphingomyelin(SM), total cholesterol and phospholipid levels elevated and the hydrogen peroxide stress test indicated increased susceptibility of both pathologic RBCs to peroxidation. Present findings indicate that IDA and thalassemla, allow for considerable amounts of oxidative damage to membrane lipids, irrespective of their etiologles, and thus point hypochromia as an important contributor for inducing lipid imbalance and RBC hemolysis.     

Differential erythrocyte agglutination pattern in normal and cancer patients with Synadenium grantii root (Hook f) lectin

In the present study the property of lectin agglutination in blood on normal and different cancer patients has been observed. The purifiedSynadenium grantii root lectin was non blood group specific and its utility as a diagnostic tool in malignancy was studied. Hemagglutination (units/ml) of red blood cells of different types of cancer was compared with the normal control's red blood cells. Out of 113 total cancer patients, only a group of 29 breast cancer patients showed significant increase in titre value (P<0.05) compared to normal control.     

Active Control of Free Paraboloidal Membrane Shells Using Photostrictive Actuators

The paraboloidal membrane shell with free boundary condition is actively controlled using photostrictive actuators which can provide contactless actuation under the illumination of ultraviolet light. The governing equations of the paraboloidal shell laminated with paired photostrictive actuators are established based on membrane approximation. The modal control actions of meridional/circumferential actuators are respectively formulated and evaluated by case studies. Constant light intensity related to the velocity of the shell is adopted, and then the governing equations are written in a closed-loop form which can be solved with Newmark-β method. Considering the multi-field coupling behavior of photostrictive actuators, time histories of transverse displacement and control light intensity are simulated and evaluated. The results show that photostrictive actuators can effectively control the vibration of the paraboloidal membrane shell, and the photostrictive actuators oriented along circumferential direction can give better control effect than photostrictive actuators placed along the meridional direction.