Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.     

An integrated and open-ended experiment

In this article we discuss an exciting experiment in non-linear dynamics. This provides an imaginative platform for bringing in chemical, physical, biological, mathematical and computational sciences together. There are implications for earth sciences as well. B M Deb is a theoretical chemist who has been designing experiments for chemistry teaching laboratories for more than thirty years. Mainak Sadhukhan, Sudarson Sekhar Sinha and Sucheta Sengupta were students of PBIR-Chemical Sciences. Ranjit Biswas is a physical chemist working on both theory and experiment in chemical dynamics.     

蛭类吸血特性和分子进化初探

将云南牛蛭（P.yunnanensis）水蛭素基因的克隆测序结果与前人所报导的20个抗凝血因子和相对应的种属线粒体细胞色素氧化酶片段I（COI）比对,构建NJ进化树,以对吸血蛭类的分子进化机制及舌蛭科和医蛭亚目吸血特性的起源进行探究．结果表明：蛭类凝血抑制因子按相似性可分为以Hirudin家族蛋白和Antistasin蛋白家族为基础的两大类群;吻蛭目和无吻蛭目的吸血特性虽然起源不同,但可能共有一套相同位点的遗传基因．     

分子伴侣、蛋白质聚集和大分子拥挤环境对蛋白质折叠的影响(英)

研究了细胞内存在的分子伴侣、蛋白质聚集和大分子拥挤环境对蛋白质折叠的影响.首先,发现分子伴侣GroEL与底物蛋白的结合有 半位 "和 全位"两种模式,它是由底物蛋白的分子形状、分子大小以及与GroEL的相互作用性质决定的.接着,发现两种不同的蛋白质一起复性时相互不干扰,提示细胞内蛋白质折叠可能不受其他蛋白聚集的影响;后又发现α 乳清蛋白的前熔球态不仅是分子伴侣也是蛋白质聚集体的作用对象.最后,研究大分子拥挤环境对蛋白质折叠热力学和动力学的影响,揭示了这种影响的复杂性和多样性.     

硅微电子技术物理极限对策探讨

根据国际半导体技术蓝图(ITRS)发布的未来半导体工艺技术预测,2016年世界集成电路主流工艺线宽为22纳米,2022年达到10纳米。届时,以硅为基础的微电子技术发展所遵循的摩尔定律将不再适用;为此,必须发展基于全新原理的新技术,以满足人类不断增长的对信息量的需求。本文首先介绍硅材料国内、外的发展现状与趋势,进而讨论后摩尔时代的微电子技术发展可能采取的对策,最后展望基于全新原理的纳米电子、分子电子、光计算和量子信息技术的发展前景。     

谈谈对气体的范德瓦耳斯方程中压强p的理解

本文将对准真实气体的范德瓦耳斯状态方程中压强p,提出作者的理解,并指出部分现行教材中对此问题阐述的不足之处。     

RAPD与AFLP分子标记的研究应用进展

通过对RAPD、AFLP两种分子标记的原理、特点及应用的综述 ,表明这两种分子标记各有其特有的优势和不足 ,可有选择地应用于不同领域 ,并展望了它们的应用前景。     

磷酸锰钴铝(MnCoAPO—5)分子筛的合成与表征

以三乙醇胺作模板剂,用水热法合成了MnCoAPO-5分子筛。用X射线衍射,电子探针,红外光谱等方法进行了结构研究,并测定了其晶胞参数和化学组成,同时对此分子筛的晶体形貌,热稳定性,吸附性能和比表面等进行了研究。证明锰和钴同时进入AlPO-5分子筛骨架而未改变结构类型。     

案例教学法在药学分子生物学教学中的应用

案例教学法(Case-Based Learning,CBL),即通过教师搜索相关案例,引导学生对案例进行分析,使学生运用所学理论知识指导应用、解决问题。将案例教学法融入药学分子生物学的教学过程中,可以激发学生的学习积极性,提高教学质量。     

取代苯胺和苯酚类化合物的结构表征与结构-活性定量相关关系研究

为了评估和测定有机污染物取代苯胺和苯酚类化合物的生物毒性 ,在分子电性距离矢量的基础上引入正辛醇 水分配系数对这类化合物进行了结构表征 ,建立了取代苯胺和苯酚类化合物的结构 -与毒性之间的定量数学模型 (R =0 96 0 ) ,并通过逐步回归分析选出 4个参数建立一新的QSAR方程 (R =0 95 8) ,这些方程可以用来进行该类化合物危害性的定量评估和预测