GC-MS法对糖李子籽中脂肪酸成分分析

对糖李子籽的脂肪酸组成和含量进行了分析测定．利用索氏提取法对果籽中的脂肪油进行提取,以KOH-CH3 OH溶液将脂肪油中的脂肪酸甲酯化,经气相色谱-质谱联用仪进行测定并分析．结果表明：糖李子籽中不饱和脂肪酸含量丰富,其亚油酸的相对百分含量为88．20％,极具开发利用价值．     

腹腔注射丁酸盐对大鼠乙酸性结肠炎的预防作用(英文)

目的:探索腹腔注射丁酸盐对防止乙酸性结肠炎的疗效。创新点:首次对大鼠进行腹腔注射丁酸盐,通过与直肠灌注和口服比较,探索三种不同给药方式对预防乙酸性结肠炎的疗效差异。方法:以40只Wistar大鼠为实验对象,分组进行连续7天的腹腔注射、直肠灌注和口服100 mg/kg丁酸钠(SB),第8天进行乙酸(AA)直肠灌注,48小时后处死。记录实验大鼠的临床症状,包括体重减少、腹泻、便血等。对结肠切片进行组织病理学观察,最后对试验数据进行统计分析。结论:腹腔注射、直肠灌注和口服丁酸盐均能明显缓解大鼠乙酸性结肠炎的炎症,其中以腹腔注射疗效最佳。     

Leaf and root glucosinolate profiles of Chinese cabbage (Brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation

Glucosinolates (GSs) are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid (JA) and salicylic acid (SA) are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage (Brassica rapa ssp. pekinensis). In this study, we applied different concentrations of methyl jasmonate (MeJA) and/or SA onto the leaf and root of Chinese cabbage to investigate the time-course interactive profiles of GSs. Regardless of the site of the elicitation and the concentrations of the elicitors, the roots accumulated much more GSs and were more sensitive and more rapidly responsive to the elicitors than leaves. Irrespective of the elicitation site, MeJA had a greater inducing and longer lasting effect on GS accumulation than SA. All three components of indole GS (IGS) were detected along with aliphatic and aromatic GSs. However, IGS was a major component of total GSs that accumulated rapidly in both root and leaf tissues in response to MeJA and SA elicitation. Neoglucobrassicin (neoGBC) did not respond to SA but to MeJA in leaf tissue, while it responded to both SA and MeJA in root tissue. Conversion of glucobrassicin (GBC) to neoGBC occurred at a steady rate over 3 d of elicitation. Increased accumulation of 4-methoxy glucobrassicin (4-MGBC) occurred only in the root irrespective of the type of elicitors and the site of elicitation. Thus, accumulation of IGS is a major metabolic hallmark of SA- and MeJA-mediated systemic response systems. SA exerted an antagonistic effect on the MeJA-induced root GSs irrespective of the site of elicitation. However, SA showed synergistic and antagonistic effects on the MeJA-induced leaf GSs when roots and leaves are elicitated for 3 d, respectively.     

Detection of Bone Metastases in Breast Cancer (BC) Patients by Serum Tartrate-Resistant Acid Phosphatase 5b (TRACP 5b), a Bone Resorption Marker and Serum Alkaline Phosphatase (ALP), a Bone Formation Marker,in Lieu of Whole Body Skeletal Scintigraphy with Technetium99m MDP

Bone metastases are a serious problem in patients with advanced cancer disease and their presence usually signifies serious morbidity prior to the patient’s death. In breast cancer patients the incidence of bone metastasis is observed to be very high at 70 %, as seen during post-mortem examination. Bone metastasis is difficult to diagnose, treat or follow clinically without radiological tools. This study was designed to evaluate the utility of a novel bone resorption marker–serum tartrate-resistant acid phosphatase 5b (TRACP5b) and the bone formation marker such as serum total alkaline phosphatase (ALP), in comparison with whole body skeletal scintigraphy with Technetium99m MDP for the diagnosis of bone metastases (BM) in breast cancer (BC) patients. This study is intended to help the clinician to diagnose bone metastasis without resorting to radiological tools, as they are not cost effective and carry the risk of radiation. Experimental design: Four groups of samples were analysed. 1st group consists 52 normal female (cancer free women), 2nd group consists 38 BC patients without bone metastasis, 3rd group consists 27 breast cancer patients with limited bone metastasis (3 or less than 3 skeletal lesions) and 4th group consists 35 breast cancer patients with extensive bone metastasis (4 or more than 4 skeletal lesions), conformed by whole body skeletal scintigraphy with Technetium99m MDP. One way ANOVA was used to compare serum TRACP5b and serum ALP among these groups. Both serum TRACP5b and serum ALP are not markedly elevated in limited bone metastasis but are strongly elevated in extensive bone metastasis (p < 0.0001). As seen in this study the biochemical bone resorption marker, serum TRACP5b, abnormally increased in extensive bone metastasis of breast cancer patients and can be used as a specific marker for bone metastasis in lieu of radiological tools.     

聚丙烯酸吸水树脂的反相悬浮聚合研究

环己烷为连续相，水为分散相，采用反相悬浮聚合法合成了聚丙烯酸高吸水性树脂，研究了碱用量，引发剂用量，交联剂用量及温度等反应条件对树脂物理状态和吸水性能的影响，制得吸水树脂最高可吸水850mL/g，吸0．9％NaCl溶液120mL/g。     

乳清酸的单扫示波极谱研究

乳清酸在０．１ｍｏｌ／ＬＨＣｌＯ４＋０．０２ｍｏｌ／ＬＮａＣｌＯ４底液中，于ＪＰ－２型示波极谱仪上，有一良好的二阶导数峰Ｅｐ＝－０．８６０±０．０１Ｖ（ＳＣＥ），浓度在１×１０－７～６×１０－５ｍｏｌ／Ｌ范围内，与峰高成线性关系，检测限为７．５×１０－８ｍｏｌ／Ｌ．实验证明，电极过程为具有催化性质的双电子可逆过程．     

微波辐射合成肉桂酸

本文介绍了微波辐射合成肉桂酸的方法,该法与传统加热法相比具有简便快捷、产率和纯度高、对环境污染小的优点。     

Serum glycoconjugates and ceruloplasmin in cancer of uterine cervix

The present study was done to assess the levels of glycoconjugates and ceruloplasmin in sera of patients with cervical cancer. Serum hexoses, hexosamines, sialic acid and fucose are elevated in a variety of inflammatory and neoplastic conditions. All the glycoconjugates, except fucose were increased in serum of patients compared to controls. Also, hexoses and sialic acid levels were high in patients with later stages of cancer compared to patients with early stage disease (P=<0.0001, P=0.03). Serum ceruloplasmin was increased in patients with early stage cancer (51.5mg/dl) and with late stage cancer (61mg/dl) compared to controls (38mg/dl). The elevated glycoconjugates may be the result of inflammatory reaction associated with neoplasia, as serum ceruloplasmin (an acute phase reactant) is also increased in these patients.     

SINGLE-SWEEP OSCILLOPOLARO-GRAPHIC ANALYSIS OF P-NITROBENZOIC ACID

ＳＩＮＧＬＥ－ＳＷＥＥＰＯＳＣＩＬＬＯＰＯＬＡＲＯ－ＧＲＡＰＨＩＣＡＮＡＬＹＳＩＳＯＦＰ－ＮＩＴＲＯＢＥＮＺＯＩＣＡＣＩＤ￥ＹｕａｎＺｈｕｏ－ｂｉｎ；ＨａｎＦｅｎｇ－ｍｅｉ；ＣｈｅｎＹｏｎｇ；ＷｕＹａｎ－ｘｉａ（ＧｒａｄｕａｔｅＳｃｈｏｏｌ，Ｃｈｉｎ...     

关于氯甲基膦酸性质的研究

介绍氯甲基膦酸的合成方法、纯品的制备和主要用途以及氯甲基膦酸的物理化学性质.重点是对该化合物的酸性进行了研究,利用电位滴定法绘出了滴定曲线.研究表明,氯甲基膦酸为二元酸,可以进行二级电离.在测定温度(27℃)下,其浓度平衡常数Kα_1和Kα_2分别为1.29×10~(-2)、6.82×10~(-7);活度平衡常数K’α_1和K’α_2分别为1.26×10~(-2)和6.51×10~(-7).