中草药对热应激蛋鹌鹑生产性能及部分血液生化指标的影响

选取100日龄健康北京蛋鹌鹑6000只,随机分成3组,A组为对照组,B、C组分别给予中草药粉剂、冲剂,按日粮的0．1％添加。试验表明：40天的试验期内,试验组与对照组相比料蛋比、产蛋总重、产蛋率、蛋破损率、死淘率、经济效益差异显著（P〈0．05）,平均蛋重、畸形蛋差异不显著（P〉0．05）;血清总蛋白、血清白蛋白、血清球蛋白、血钙、血磷、碱性磷酸酶、总甲状腺激素（T3、T4）升高（P〈0．05）,血糖、乳酸脱氢酶、皮质醇（Cor）下降（P〈O．05）,雌二醇（E2）变化不显著（P〉0．05）。     

引入虚拟变量的商业银行信用风险压力测试研究

本文引入代表农业银行不良贷款剥离的虚拟变量(D),把全国主要商业银行的不良贷款率的Logit转换指标Y作为承压指标,用实际GDP增速、消费物价指数CPI、广义货币供应量M2增速、房屋价格指数四个宏观因素作为解释变量,建立回归模型,并通过向量自回归(VAR)模型建立变量之间的相互关系。以此模型系统为基础,对商业银行不良贷款进行了不同假设场景压力测试,测试范围覆盖了4个季度,发现GDP增速放缓、CPI骤降、广义货币供应增速放缓以及房屋价格指数升高均会使得不良贷款率短期内不同程度的升高,但从长期看,商业银行能通过模型系统内相互作用逐渐消化宏观因素变化冲击带来的影响。     

高职院校学生德育发展的SWOT分析——基于“后喻文化”视角

当代社会已迈入后喻文化时代,即年长一代逐渐转变为向青年群体学习知识、获取信息,并以"文化反向社会化"为最显著特点的时代。在这一背景下,后喻文化对高职院校学生的德育带来了一系列不可忽视的影响。运用SWOT方法详实地分析其对高职学生德育产生的优势和劣势,带来的机会和威胁,并对出现的诸多问题进行策略探究。     

“狂飚年代”三部曲四人谈

"狂飚年代"是香港作家寒山碧的代表作,包括《还乡》《逃亡》《他乡》三部长篇小说。从2001年《还乡》出版,到2013年《逃亡》和《他乡》最终完成,"狂飚年代"的创作历时12年,对作者来说,这不仅是他费时最多的作品,也是他的心血之作。2013年7月中旬,寒山碧先生(下文简称韩)回故乡文昌,海南师范大学中国现当代文学学科邀其与本学科部分师生座谈,并与邵宁宁教授(下文简称邵)、房福贤教授(下文简称房)、扬州大学文学院孙德喜副教授(下文简称孙)一起,就有关问题进行了坦率、真诚的对话、交流。以下即有关座谈内容的概要。     

后喻文化时代高校思想政治教育的应对策略

后喻文化时代构筑了高校思想政治教育特殊的文化教育环境:学生主体意识强烈,反叛精神鲜明;文化多元,价值观混乱;教师传统权威的消解。这对高校思想政治教育提出了应对性的要求:思想政治教师要正确定位自己的角色,更新教育观念;要更新教育内容,提高学生的媒介素养,重视情感教育;要提高使用现代科技的能力,注重信息的收集、分析和处理;要创新教育方式,将正式教育和生活化教育结合起来。     

Oral Nicotine Induces Oxidative Stress and Inflammation but Does Not Subvert Tumor Suppressor and DNA Repair Responses in Mice

Nicotine, responsible for the addictive properties of tobacco, is widely used in nicotine replacement therapy for tobacco use cessation. We investigated the time-dependent effect of treatment with nicotine on the tumor suppressor, DNA repair and immune responses. Swiss Albino mice (laca strain) of both sexes received nicotine dissolved at a dose of 100 µg/ml in 2% sucrose for 24 weeks, by oral gavage, while age- and gender-matched controls received only 2% sucrose for the same period. Nicotine-treated and control mice were sacrificed 6, 16 and 24 weeks post-treatment, and their tissues evaluated for alterations in histology, oxidative stress, TNF-α levels, nitric oxide (NO) and myeloperoxidase (MPO) release, tumor suppressor response and DNA repair response. Statistical significance of results was determined using Students’ t test. The tissues of nicotine treated mice exhibited a large number of multinucleated and binucleated cells, enlarged nuclei and non-uniform distribution of cells, significant increase in expression of TNF-α gene and serum TNF-α, and time-dependent significant increase in lipid peroxidation, protein carbonylation, NO and MPO release when compared to age-and gender-matched controls. The mRNA expression of the tumor suppressor gene p53, its primary regulator Mdm2, and the DNA repair genes Brca2 and Ape1 were significantly elevated, but the corresponding protein levels remained largely unaltered. In conclusion, treatment with nicotine caused oxidative stress and inflammation which can cause widespread cellular damage from the very onset of treatment, without subverting the tumor suppressor and DNA repair responses.Electronic supplementary materialThe online version of this article (10.1007/s12291-020-00903-8) contains supplementary material, which is available to authorized users.     

Copper and Zinc Status in Psoriasis: Correlation with Severity

Psoriasis is a common and an inflammatory skin disease. Trace elements play an important role in the skin metabolism such as keratinisation and melanin formation as well as immunological and inflammatory reactions. Henceforth, the present study was aimed to evaluate the essential metals with special reference to Cu and Zn. In this study, 72 psoriatic patients and 50 controls were enrolled. On the basis of PASI score psoriatic group comprising of 48 mild and 24 severe psoriatic patients were grouped. The serum trace elements analysis in the subjects revealed that serum Cu levels and Cu/Zn ratio was significantly higher in the psoriatic patients as compared to controls. Interestingly, severity of the psoriasis was well correlated with the serum Cu levels. Taking together, all these findings suggest that Cu may be a major culprit in the pathogenesis as well as in the severity of the disease.     

Identification and characterization of profilin gene family in rice

BackgroundProfilin proteins (PRFs) are small (12–15 kD) actin-binding protein, which play a significant role in cytoskeleton dynamics and plant development via regulating actin polymerization. Profilins have been well documented in Arabidopsis, Zea mays L. as well as Phaseolus vulgaris, however no such fully characterization of rice (Oryza sativa L.) profilin gene family has been reported thus far.ResultIn the present study, a comprehensive genome-wide analysis of rice PRF genes was completed and three members were identified. OsPRF1 and OsPRF2 shared 98.5% similarity (6 nucleotide divergence), but the deduced amino acid sequences of OsPRF1 and OsPRF2 are fully identical. In contrast, the OsPRF3 presents relatively lower similarity with OsPRF1 and OsPRF2. Phylogenetic analysis also support that OsPRF1 has a closer relationship with OsPRF2. Expression pattern analysis revealed the differential expression of OsPRFs in tissues of mature plant, which suggested the potential spatial functional specificity for rice profilin genes. Subcellular localization analysis revealed the OsPRFs were localized in cytoplasm and nucleus and all of them could bind actin monomers. Furthermore, abiotic stresses and hormones treatments assay indicated that the three OsPRF genes could be differentially regulated, suggesting that OsPRF genes might participate in different stress processes in rice.ConclusionsTaken together, our study provides a comprehensive analysis of the OsPRF gene family and will provide a basis for further studies on their roles in rice development and in response to abiotic stresses.How to cite: Zhang Y, Dong G, Wu L, et al. Identification and characterization of profilin gene family in rice. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.004.     

虚无中的抗争——论海明威的“准则英雄”

海明威塑造了著名的“准则英雄”来对抗一个“异我”,传统价值观丧失,混乱不堪的世界。评论家大多认为“准则英雄”体现了坚强,无畏,充满勇气等性格特点。但实际上,海明威式英雄的共性并非性格特点,而是相同的人生观和世界观。他们的遭遇使他们意识到生存的荒谬和虚无,但他们坚信“要在重压之下保持优雅风度”为基准的律令,不屈不挠地和虚无作斗争,寻找人生的坐标和意义。文章通过对海明威四部长篇小说的文本分析,力求证实“准则英雄”这一人物形象的塑造经历了一个不断完善的过程;同时,也揭示“准则英雄”的抗争对现代人生存的哲学意义。     

高职院校90后学生干部队伍建设的思考与实践

学生干部是高校校园各类活动组织者,是营造校园文化的主体,也是高校学生工作老师的得力助手。而现在,随着90后成为大学生主体,90后学生干部已然成为学校学生干部的中坚力量。由于各种原因,这支队伍存在诸多方面的问题,要做好学生工作,高职院校要从选拨、培养、教育、考核四个方面重视和加强90后学生干部队伍建设,推动高职院校学生工作再上新台阶。