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61.
Liu F Zhong H Liang JY Fu P Luo ZJ Zhou L Gou R Huang J 《Journal of Zhejiang University. Science. B》2010,11(12):905-911
In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein. 相似文献
62.
维普数据库Z39.50服务端系统设计方案 总被引:1,自引:0,他引:1
介绍了维普数据库Z39.50服务端系统的设计方案,该方案有两条解决途径:基于TCP/IP的Z39.50服务端系统和基于HTTP的SRU服务端系统,笔者将这两种思路做了比较。本系统突破了传统Z39.50协议只运用于对书目数据的提供,而是提供电子期刊数据,这是本方案的特色所在。 相似文献
63.
芦军志 《体育科技文献通报》2010,18(11):80-81,101
运用文献资料和逻辑分析法,研究街头篮球起源及发展的文化背景,分析中国街头篮球的发展历程、现状及发展方向,探讨其对中国职业篮球发展的影响。 相似文献
64.
陈世军 《福建工程学院学报》2018,(4):365-371
借鉴求线性矩阵方程组同类约束解的MCG算法(修正共轭梯度法),建立了求多个未知矩阵的线性矩阵方程组的一种异类约束解的MCG1-3-5算法,证明了该算法的收敛性。该算法不仅可以判断矩阵方程组的异类约束解是否存在,而且在有异类约束解,且不考虑舍入误差时,可在有限步计算后求得矩阵方程组的一组异类约束解;选取特殊初始矩阵时,求得矩阵方程组的极小范数异类约束解。同时还能求取指定矩阵在该矩阵方程组异类约束解集合中的最佳逼近。算例表明,该算法有效。 相似文献
65.
Benjamin Xie Dastyni Loksa Greg L. Nelson Matthew J. Davidson Dongsheng Dong Harrison Kwik 《Computer Science Education》2019,29(2-3):205-253
ABSTRACTBackground and Context: Current introductory instruction fails to identify, structure, and sequence the many skills involved in programming.Objective: We proposed a theory which identifies four distinct skills that novices learn incrementally. These skills are tracing, writing syntax, comprehending templates (reusable abstractions of programming knowledge), and writing code with templates. We theorized that explicit instruction of these skills decreases cognitive demand.Method: We conducted an exploratory mixed-methods study and compared students’ exercise completion rates, error rates, ability to explain code, and engagement when learning to program. We compared material that reflects this theory to more traditional material that does not distinguish between skills.Findings: Teaching skills incrementally resulted in improved completion rate on practice exercises, and decreased error rate and improved understanding of the post-test.Implications: By structuring programming skills such that they can be taught explicitly and incrementally, we can inform instructional design and improve future research on understanding how novice programmers develop understanding. 相似文献
66.
运用李对称方法得到了(3+1)维Kadomtsev Petviashvili Boussinesq(KPB)方程的对称和约化方程。进而,利用齐次平衡原理和椭圆函数方法得到了方程的精确解。最后,给出了该方程的守恒律。 相似文献
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69.
研究女性胆固醇随年龄变化的趋势.采集大最女性胆固醇数据,以10岁为年龄段,计算每个年龄段健康女性胆固醇平均值,并以女性70岁以下6个年龄段的胆固醇为原始数列,建立了女性胆固醇随年龄变化的灰色系统(GM(1,1))模型预测.后验差检验合格,预测70~79岁、80以上年龄段的女性胆固醇.预测分析结果表明,灰色系统GM(1,... 相似文献
70.
BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006 相似文献