茶树中咖啡酰辅酶A氧甲基转移酶的基因克隆及其催化活性研究(英文)

目的:从茶树中克隆一种可以催化表没食子儿茶素没食子酸酯(EGCG)生成甲基化EGCG的酶——咖啡酰辅酶A氧甲基转移酶(CCo AOMT),实现甲基化EGCG的酶学合成,为甲基化EGCG的进一步开发利用提供理论依据和技术指导。创新点:本研究首次从茶树中克隆了一条CCo AOMT基因组序列;分析了CCo AOMT基因在不同茶树品种和不同成熟度茶鲜叶中的基因表达规律;证明了CCo AOMT具有催化合成甲基化EGCG的生物活性。方法:采用聚合酶链式反应(PCR)和序列分析获得CCo AOMT的编码序列和基因组序列;采用高效液相色谱-四级杆-飞行时间串联质谱技术(HPLC-QTOF-MS)分析酶促反应生成的甲基化EGCG产物(图4);采用实时荧光定量PCR分析CCo AOMT基因的表达差异(图5)。结论:本研究从茶树中克隆了CCo AOMT基因的编码序列(738 bp)和基因组序列(2678 bp),明确了该基因具有4个内含子和5个外显子;揭示了CCo AOMT可以催化EGCG生成EGCG4"Me、EGCG3"Me和EGCG3’Me等多种甲基化产物;证明了CCo AOMT具有催化生成甲基化EGCG的活性;并发现该基因的表达量高低与茶鲜叶的成熟度呈正相关关系。     

教学比赛:教师个体成长的阶梯——基于优秀教师PCK建构的个案研究

教师在参加教学比赛过程中,课程改革的推动作用、教研活动的促进作用、名师培养机制和教师合作共同体的支撑,对教师个体专业发展具有非常重要的意义。因此,要加强教学比赛的示范引领作用,开展教研活动,激发教师PCK生成,促进教师专业发展,完善教师在职培养制度,为教师个体专业发展保驾护航。     

Effect of microcystin-LR on protein phosphatase 2A and its function in human amniotic epithelial cells

Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However, the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation. To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity of MCLR.     

MBA“运营管理”教学面临的困惑与思考

“运营管理”是MBA的核心课程之一。MBA“运营管理”教学面临研究对象的变化与教材内容体系的混乱、MBA 学员背景的差异与需求的多元化、网络信息技术对企业运营系统与运营活动的影响等困惑,直接影响MBA“运营管理”课程的教学效果。MBA“运营管理”课程可以从课程内容拆分、制造企业运营与服务企业运营分离、学员根据工作需要选修等措施进行尝试性的改革与探索。     

Girls’ active identities: navigating othering discourses of femininity,bodies and physical education

Within physical education and sport, girls must navigate discourses of valued athletic and gendered bodies that marginalise or ‘other’ non-normative performances through systems of surveillance and punishment. The purpose of this paper is to share girls’ perspectives on how these discourses affected their gender performances and activity engagement. Students aged 13–14 in one ethnically diverse UK secondary school were invited to create a photo diary of the physical activities they engaged in. Photo-elicitation interviews in small groups followed. The girls positioned themselves as physically active but had to carefully manage their activity choices and gender performances in a single-sex physical education environment that regulated deviation from the fit, slender, girly-girl. Although the girls demonstrate the difficulty of resisting, they indicate moments of positioning themselves against norms that suggest the possibilities of shifting gendering processes. The paper points out the importance of listening to ‘other’ girls’ narratives in building positive physical education engagements.     

公益活动与民航高职院校学生职业素质养成教育的契合性研究

公益活动作为一种服务于公共利益的社会活动,具有实践性、公益性、扶弱性等特点,与民航高职院校学生的职业素质养成具有契合的可能性。广州民航职业技术学院作为一所行业院校,通过“亚运会”、“一奖两会”、“世广会”、“爱心服务队”等公益活动和组织,实现了两者的契合。为进一步促进契合的实际效果,应在深度合作、体系建设、激励制度等方面着力推进。     

概念整合理论下的中国英语的词汇翻译研究

国内对中国英语以及对中国英语中的词汇的翻译研究发展了近30年的时间,不同的人对中国英语中词汇的翻译问题所持有的态度也不同,传统的翻译方法自身存在的问题并不能够凸显中国英语作为一种具有中国特色的英语变体的特点,概念整合理论以其强大的认知阐释力证明了中国英语的词汇翻译是一种中国人独有的认知活动,从而进一步证明了中国英语是一种普遍存在的具有中国特色的英语变体。     

休闲与娱乐：西南民族村寨体育文化特征分析

西南地区是我国民族成分较多的区域之一,不仅民族成分众多,而且少数民族传统体育文化资源的数量较为庞大。其民族村寨体育无论是竞赛类,还是表演性质的竞技类、技巧类和综合类项目,均体现出较强的休闲娱乐特征,而且随着社会生活的演变,我们今天能够看到的民族村寨体育文化,其原有的功能已在逐渐退化,反而是休闲、娱乐与健身的功能日趋突出,它为村寨居民提供认同感和持续感,显示出我国文化的多样性和创造力,成为人们生活的重要组成部分。     

Analysing Creative Design Process: A Set of Tools to Understand Activity in its Socio-cultural and Historic Context

Previous theoretical frameworks used to research and explain creative design processes tend to privilege individual expression and not address the context in which the process occurs. This is problematic due to the ways in which creative activities are embedded in and shaped by socio-cultural and historic contexts. In this article we focus on the ways in which cultural historic activity theory (CHAT) and its analytical activity systems can be used to reveal creative processes in context. We draw on the tools and concepts of CHAT and data from a study of visual communication design (VCD) students and lecturers situated within a transnational higher education (TNHE) context at a university in China. An analytical framework was constructed to research practices used in creative design process learning. The dynamic nature of CHAT offers design process research methods a set of analytical tools to capture the powerful parts played by artifacts and interactions within specific sociocultural and historic learning contexts.     

太阳活动和大气涛动对天山南北气温波动的综合影响

利用天山南北33个气象站点1961-2012年逐日气温数据,运用滑动平均、线性倾向估计、Mann-Kendall非参数检验等气候诊断方法,分析了近52年天山南北气温变化趋势、突变特征,探讨了太阳活动、主要大气涛动对气温波动的综合影响。结果表明:①近52年天山南北气温暖化趋势明显。3个分区增温速率关系为:天山南坡(0.256℃/10a)天山北坡(0.259℃/10a)天山东段(0.446℃/10a);②天山南北气温变化与太阳活动波动具有一定的规律性,表现为:第19-20周期"反相关",第21-22周期和23周期上升段"正相关",第23周期下降段和第24周期"反相关";③ENSO事件对天山南北气温变化影响相对较弱,且ENSO事件强度与天山南北气温年际变化相关性存在不稳定性;④北极涛动(AO)和北大西洋涛动(NAO)与天山南北气温变化相关性较高且存在季节差异。其中,北极涛动(AO)主要影响天山南北冬季和春季气温变化,空间上天山北坡响应明显于南坡。北大西洋涛动(NAO)主要影响夏季气温变化,空间上夏季气温影响区域广泛,冬季气温影响主要集中在天山南北坡边缘地带;春季气温影响区域主要集中在天山北麓。