基于ANP的毕业要求达成度评价解析

毕业要求达成度是基于成果导向教育模式(Outcomes--based Education,OBE)的核心概念,对其评价的合理性直接影响到OBE教育模式的实施效果。传统的相关评价中存在教育过程性因素考查不充分、指标不独立引起的信息损失过多、评价方法使用受限、评价结果不准确等问题,而基于网络层次分析法(ANP)的毕业要求达成度评价可在一定程度上解决这些问题。该评价方法不仅有效融入了教育全过程因素,而且充分考虑了各指标间的关联性,能够反映出全部指标的相对重要度,减少了信息损失,更符合OBE中毕业要求达成度的评价要求。     

基于成果为本的高职商务英语专业毕业设计模式构建

毕业设计是高职教育的一个重要的实践性教学环节,但目前商务英语专业毕业设计中还存在许多问题,与商务英语专业人才培养目标有一定的偏差。本文针对上述问题,结合我校商务英语专业毕业设计实践,提出构建基于成果为本的毕业设计模式,以保证毕业设计的质量,并期望能为高职毕业设计改革提供思路。     

Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing

Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification.In this study,a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin(PVC)sheet.This modified method is named EZ-D,for EASY DNA extraction.Compared with the original cetyl trimethylammonium bromide(CTAB)method,DNA extracted by EZ-D is more efficient in polymerase chain reaction(PCR)amplification due to the more stable performance of the EZ-D stick.The EZ-D method is also faster,easier,and cheaper.PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples.A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL.Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80%in GC content.EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues.Moreover,when EZ-D was combined with the loop-mediated isothermal amplification(LAMP)method,DNA identification of biological samples could be achieved without the need for specialized equipment.As an optimized DNA purification method,EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.     

地质超前预报系统在栋梁坡隧道施工中的应用

基于地震反射波法超前预测系统的原理及主要技术手段,以栋梁坡隧道工程为实例,针对隧道复杂的地质条件,施工过程中将超前地质预报和地质素描调查相结合,先对隧道全段范围内地形、地层岩性等进行全面核查,然后结合核查结果运用地震反射波法预报掌子面前方的节理裂隙发育情况,围岩完整性等地质信息.开挖后验证情况与预测结果基本一致,为后续...     

Testing and ideology: policy debates about literacy assessments for Colorado’s bilingual students

ABSTRACT

The Colorado Reading to Ensure Academic Development Act requires grade-level attainment in literacy in English for students in grades K-3. Its practical outcome, however, has been to pressure schools with bilingual programs to shift their instructional language allocations towards more English in the early grades. Proposed rule revisions debated by the state Board of Education sought to facilitate testing in students’ language of instruction for those in bilingual programs. Analysis of written and verbal opposition to the proposed rule revisions demonstrates the persistence of insidious ethnoculturalist discourses opposing bilingual education as well as the cooptation of liberal multiculturalist discourses that, while framing bilingualism as an asset, argue that it is underdeveloped in bilingual programs that do not include English instruction from the outset. Given that bilingual instruction has demonstrated time and again that it benefits students acquiring English in schools, such new discursive turns pose a threat that must be recognized to fend off further legislative and regulatory attempts against bilingual education.     

雌激素受体(ER)与基因蛋白(P53、erbB-2)在胃癌中表达的研究

目的:探讨ER、erbB-2和P53在胃癌表达及相关性。方法:取胃癌手术标本119例,SP免疫组织化学染色。结果:ER、erbB-2和P53在胃癌表达率分别为63.87％,75.51％和73.11％;三者同时阳性者31.93％;ER与P53同时阳性者37.82％;ER与erbB-2同时阳性者33.61％;ER在高分化组阳性率高于低分化组(P<0.05);而erbB-2和P53在低分化组阳性率高于高分化组。70岁以上组P53、ER和erbB-2表达率均较高。结论:ER、P53和erbB-2在胃癌中表达率与癌瘤分化程度相关。P53和erbB-2有相关性,而且它们是胃癌预后不良的指标。     

2-（1，2-亚乙／1，3-亚丙二硫）亚甲基-3-羰基-N-芳（烷）基丁酰胺制备的新方法

以易制备的2-（1,2-亚乙／1,3-亚丙二硫）亚甲基-3-羰基丁酸1为原料,经与氯化亚砜的氯化反应后,与胺反应成功地以较好产率（40．5％-72．0％）合成重要的有机合成中间体2-（1,2-亚乙／1,3一亚丙二硫）亚甲基-3-羰基-N-芳（烷）基丁酰胺3．     

Polychlorinated dibenzo-p-dioxins and dibenzofurans in flue gas emissions from municipal solid waste incinerators in China

Polychlorinated dibenzo-p-dioxins and furans （PCDD/Fs） emissions in flue gas from two types of municipal solid waste incinerators （MSWIs） most commonly used in China were investigated in this study. The selected incinerators include two grate-type MSWIs： MSWI-A （350 t/d） and MSWI-B 050 t/d）, and two fluidized bed MSWIs： MSWI-C （400 t/d） and MSWI-D （400 t/d）, which are all equipped with semi-dry lime scrubber and bag filter except MSWI-D equipped with cyclone and wet scrubber （WS） as air pollutant control device （APCD）. Results indicated that the emission concentration and the international toxic equivalents （I-TEQs） of the PCDD/Fs from the stacks were in the range of 1.210-10.273 ng/Nm^3 and 0.019-0.201 ng I-TEQ/Nm^3, respectively. They were greatly lower than the emission regulation standard of PCDD/Fs in China （1.0 ng I-TEQ/Nm^3）. However, only the PCDD/Fs emission level from MSWI-C was below 0.1 ng I-TEQ/Nm^3. Although the homologue profiles were distinct, the contributions of the 2,3,7,8-subsituted congeners to the total I-TEQ were similar among all the investigated MSWIs. Two major 2,3,7,8-substituted congeners, 2,3,4,7,8-PeCDF and 1,2,3,7,8-PeCDD, account for 47% and 9% （average values） of the total I-TEQ values, respectively. The correlation between PCDD/Fs levels and composition of flue gas was also discussed.     

6-甲基-2-（α-羟甲基）-1H-苯并咪唑荧光增敏探针法测定蛋白质

以6-甲基-2-（α-羟甲基）-IH-苯并咪唑（MHMBM）作为荧光探针,利用蛋白质对MHMBM荧光增敏作用,建立了测定人血清总蛋白的新方法。结果表明,在中性水溶液中,MHMBM的荧光强度与牛蛋白（BSA）的浓度呈良好的线性关系,相关系数（r）为0．9995,检出限2．3μg／mL,线性范围4．6—60．0μg／mL。本方法用于测定两种样品溶液中的总蛋白,RSD（n=6）分别为1．7％和1．3％,回收率95．5％和98．6％。