情报发布系统知识转移研究

针对情报发布实施知识转移的薄弱环节,从系统工程角度出发,简述情报发布系统知识管理体系的基本功能,提出基于知识流的情报发布知识管理体系框架和情报发布知识转移的原则,重点阐述情报发布过程中知识转移的框架模型,并对知识管理体系框架下情报发布知识转移的发展趋势进行了初步探讨.     

利用XML实现图书馆Web数据库的动态发布

介绍了XML以及基于XML的三层C/S模型，探讨了利用XML实现图书馆Web数据库动态发布的设计思想，并通过示例展示了基于XML的图书馆Web数据库动态发布的具体实现。     

灵魂的绞杀与欲望的宣泄--中日当代小说的人性与精神分析

处于同一文化圈的中、日当代小说,在双重人格的刻画和复杂人性的描写方面,有许多相同和可比之处.日本一些当代小说受到复杂的文化影响,中国儒家文化和佛教文化使他们的文化性格处于隐思和内向,而西方基督教文化和精神分析学又使他们具有原罪意识和原欲的冲动,神道教和武士道精神并未完全消亡.在现代社会中,文化的冲突更加激烈,致使人的灵魂的绞杀更加深重,于是人物命运的指向较多的是走向死亡.中国小说在描写人的复杂人性时,更多是在于表层欲望的宣泄,较少触及人的精神层面,应当说中国作家对复杂人性的描写要肤浅一些.     

pH值敏感水凝胶的合成及药物缓释性能研究

聚乙二醇(PEG)与丙烯酰氯反应合成聚乙二醇双丙烯酸酯(PEGDA)，然后以PEGDA、N—乙烯基—2—此咯烷酮(NVP)及丙烯酸为主要原料，N，N^ —亚甲基双丙烯酰胺(BIS)为交联剂，偶氮二异丁腈(AIBN)为引发剂，采用自由基聚合法合成了对pH值变化敏感的N—乙烯基—2—吡咯烷酮—丙烯酸—聚乙二醇双丙烯酸酯交联共聚物(po1y[NVP-AA-PEGDA])水凝胶．利用该共聚物为载体对抗癌药5—氟脲密啶(5—FU)进行包埋，并在SIF及SGF缓冲溶液中于37℃进行体外释药研究．结果表明，在pH值较小的缓释介质中药物释放曲线较平缓，释药周期较长，同时最终的平衡释药百分数也较小；随着样品中PEGDA量的减少，释药曲线趋于平缓，释药周期变长，最终的平衡释药百分数成小．     

出口垄断协议豁免制度研究

我国出口垄断协议豁免制度虽然确立,但这种简单与原则性的规定缺乏可操作性。因此文章认为,应设置反垄断豁免的审批等程序规范,实行反垄断豁免的公示和监督制度,规定影响我国市场的外国出口垄断协议行为的调查和控制制度,明确出口垄断协议豁免行为的职能机构。     

Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil

BackgroundSuper-paramagnetic iron oxide nanoparticles (SPION) contain a chemotherapeutic drug and are regarded as a promising technique for improving targeted delivery into cancer cells.ResultsIn this study, the fabrication of 5-fluorouracil (5-FU) was investigated with loaded Dextran (DEX-SPION) using the co-precipitation technique and conjugated by folate (FA). These nanoparticles (NPs) were employed as carriers and anticancer compounds against liver cancer cells in vitro. Structural, magnetic, morphological characterization, size, and drug loading activities of the obtained FA-DEX-5-FU-SPION NPs were checked using FTIR, VSM, FESEM, TEM, DLS, and zeta potential techniques. The cellular toxicity effect of FA-DEX-5-FU-SPION NPs was evaluated using the MTT test on liver cancer (SNU-423) and healthy cells (LO2). Furthermore, the apoptosis measurement and the expression levels of NF-1, Her-2/neu, c-Raf-1, and Wnt-1 genes were evaluated post-treatment using flow cytometry and RT-PCR, respectively. The obtained NPs were spherical with a suitable dispersity without noticeable aggregation. The size of the NPs, polydispersity, and zeta were 74 ± 13 nm, 0.080 and −45 mV, respectively. The results of the encapsulation efficiency of the nano-compound showed highly colloidal stability and proper drug maintenance. The results indicated that FA-DEX-5-FU-SPION demonstrated a sustained release profile of 5-FU in both phosphate and citrate buffer solutions separately, with higher cytotoxicity against SNU-423 cells than against other cells types. These findings suggest that FA-DEX-SPION NPs exert synergistic effects for targeting intracellular delivery of 5-FU, apoptosis induction, and gene expression stimulation.ConclusionsThe findings proved that FA-DEX-5-FU-SPION presented remarkable antitumor properties; no adverse subsequences were revealed against normal cells.How to cite: Mahdia SA, Kadhimb AA, Albukhaty S, et al. Gene expression and apoptosis response in hepatocellular carcinoma cells induced by biocompatible polymer/magnetic nanoparticles containing 5-fluorouracil. Electron J Biotechnol 2021;52. https://doi.org/10.1016/j.ejbt.2021.04.001     

Improved myocardial perfusion and cardiac function by controlled-release basic fibroblast growth factor using fibrin glue in a canine infarct model

Objective: Angiogenic therapy is emerging as a potential strategy for the treatment of ischemic heart disease but is limited by a relatively short half-life of growth factors.Fibrin glue (FG) provides a reservoir for controlledrelease of growth factors.The aim of this study was to evaluate the effects of basic fibroblast growth factor (bFGF) incorporating FG on angiogenesis and cardiac performance in a canine infarct model.Methods: Acute myocardial infarction was induced by ligation of the left anterior descending coronary artery (LAD).Group I (n=6) underwent ligation of LAD alone.In Group II,transmural channels were created in the infarct area (n=6).In Group III,nontransmural channels were created to locate FG cylinders containing bFGF (n=6).Eight weeks after operation,myocardial perfusion was assessed by single photon emission computed tomography,cardiac function by echocardiography,and vascular development by immunohistochemical staining.Results: Total vascular density and the number of large vessels (internal diameter ≥50 μm) were dramatically higher in Group III than in Groups I and II at eight weeks.Only the controlled-release group exhibited an improvement in regional myocardial perfusion associated with lower defect score.Animals in Group III presented improved cardiac regional systolic and diastolic functions as well as global systolic function in comparison with the other two groups.Conclusions: Enhanced and sustained angiogenic response can be achieved by controlled-release bFGF incorporating FG within transmyocardial laser channels,thus enabling improvement in myocardial perfusion and cardiac function.     

智能型断路器的研制

脱扣器是断路器的核心元件,脱扣器的智能化可以大大地推动断路器智能化的发展进程。首先介绍了智能断路器的特点、现状和发展趋势等,然后根据其性能、原理、特点入手,分步对其结构零部件进行设计。智能脱扣器是设计的重点,利用微电子技术及数字信号处理技术,采用数字信号处理器（DPS芯片）作为主控单元,来对其它外围电路进行控制。最后,对软件程序的进行设计,设计的重点是：快速傅立叶变换在定点DSP上的实现,并给出相应的实验结果。     

教育功能释放的三重境界

论述了教育的功能,认为本体功能是培养人,教育的其他功能都只是其派生功能。依据教育的主体——人的“存在状态”,将教育功能的释放分成生存境界、生活境界和生命境界。分析了在对人的培养和对社会发展两方面分别体现为个体谋生功能、“成事”功能;个体乐生功能、“成人”功能;个体享用功能、“成己”功能。这三层境界是相互交织、相互转化的螺旋上升过程;是追求教育功能从有用到有意义,从有限到无限的过程。     

构建MARC元数据的Web Service

构建MARC的Web Service将成为网络环境下发布MARC元数据非常重要的方式之一。本文对Web Service进行介绍，探讨构建MARC元数据的Web Service的优势，并通过程序实例展示MARC元数据Web Service的具体实现。