Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system

INTRODUCTION Aqueous two-phase system (ATPS) is widely used in biochemistry and biotechnology for purifica- tion of proteins (Diamond and Hsu, 1992; Balasubramaniam et al., 2003), enzymes (Pan et al., 2001; Bim and Franco, 2000), amino acids (Li et al., 2002), antibiotics (Yang et al., 1994; Guan et al., 1994), nucleic acids (Ohlsson et al., 1978; Kimura, 2000), aroma compounds (Marco et al., 2000) and lactic acid (Planas et al., 1998). ATPS is mainly composed of two-incompatible p…     

局域弯曲液面的光学特性分析

研究了强度均匀分布的平行光束照射局域上（下）弯曲液面时，其反射光场及该反射光场经透镜后的透射光场的分布特点，发现上弯曲液面的反射光场为椭圆形暗场，下弯曲液面的反射光场为星形亮斑。而它们的透射光场恰好类似于对方的反射光场。对这些特点的形成进行了讨论，同时对上弯曲液面的反射光场进行了计算。     

论刚体对坐标轴转动惯量的平移和旋转变换

本文导出了刚体对坐标轴的转动惯量的平移和旋转变换一般式。     

Visible region extraction from a sequence of rational Bézier surfaces

A method for computing the visible regions of free-form surfaces is proposed in this paper. Our work is focused on accurately calculating the visible regions of the sequenced rational Bézier surfaces forming a solid model and having coincident edges but no inner-intersection among them. The proposed method calculates the silhouettes of the surfaces without tessellating them into triangle meshes commonly used in previous methods so that arbitrary precision can be obtained. The computed silhouettes of visible surfaces are projected onto a plane orthogonal to the parallel light. Then their spatial relationship is applied to calculate the boundaries of mutual-occlusion regions. As the connectivity of the surfaces on the solid model is taken into account, a surface clustering technique is also employed and the mutual-occlusion calculation is accelerated. Experimental results showed that our method is efficient and robust, and can also handle complex shapes with arbitrary precision. Project supported by the National Basic Research Program (973) of China (No. 2002CB312106) and the National Natural Science Foundation of China (Nos. 60533070, and 60403047). The third author was supported by the project sponsored by a Foundation for the Author of National Excellent Doctoral Dissertation of China (No. 200342) and a Program for New Century Excellent Talents in University (No. NCET-04-0088), China     

A NEW ALGORITHM FOR FAST HIDDEN SURFACE REMOVAL OF COMPLEX OBJECTS

ＡＮＥＷＡＬＧＯＲＩＴＨＭＦＯＲＦＡＳＴＨＩＤＤＥＮＳＵＲＦＡＣＥＲＥＭＯＶＡＬＯＦＣＯＭＰＬＥＸＯＢＪＥＣＴＳＦｕＹｕｑｉｎｇ（富煜清）；ＨｕａｎｇＪｉｎｆａ（黄金法）；ＬｉｎＦｅｎｇ（林峰）（ＤｅｐａｒｔｎｉｅｎｔｏｆＲａｄｉｏＥｎｓｉｎｅｅｒｉ...     

自组装膜的表面反应及自组膜的构筑

界面反应已成为界面自组装构筑的一个重要反应方面.从自组装膜的形成及自组装膜的气相、液相、膜上分子交联反应以及膜表面大分子寡聚体的设计组装等几个方面的表面反应对近年来自组装膜构筑方面的进展予以分析和综述.引用文献23篇.     

Application of response surface methodology in medium optimization for pyruvic acid production of <Emphasis Type="Italic">Torulopsis glabrata</Emphasis> TP19 in batch fermentation

Response surface methodology （RSM） was used to optimize the fermentation medium for enhancing pyruvic acid production by Torulopsis glabrata TP19. In the first step of optimization, with Plackett-Burman design, ammonium sulfate, glucose and nicotinic acid were found to be the important factors affecting pyruvic acid production significantly. In the second step, a 23 full factorial central composite design and RSM were applied to determine the optimal concentration of each significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the critical components were obtained as follows： ammonium sulfate 0.7498 （10.75 g/L）, glucose 0.9383 （109.38 g/L） and nicotinic acid 0.3633 （7.86 mg/L） with a predicted value of maximum pyruvic acid production of 42.2 g/L. Under the optimal conditions, the practical pyruvic acid production was 42.4 g/L. The determination coefficient （R^2） was 0.9483, which ensures adequate credibility of the model. By scaling up fermentation from flask to jar fermentor, we obtained promising results.     

Development of integrated precision vibration-assisted micro-engraving system

A novel precision vibration-assisted micro-engraving system was developed by the integration of fast tool servo and ultrasonic elliptical vibration system, in which the flexure hinge was designed to avoid backlash and PID control algorithm was established to guarantee specific precision. Apart from experimental validation of the performance of the system, various micro-V-grooves cutting experiments on aluminum alloy, ferrous material and hard cutting material were performed, in which Kistler force sensor was used to measure cutting force. Through experiments, it was clear that the vibration-assisted micro-engraving system can ensure good quality of micro-V-grooves and reduce cutting force by about 60% compared with traditional removal process without ultrasonic vibration.     

机械力化学技术制备磷酸法活性炭及其碘吸附性能的研究

研究采用机械力化学技术制备了吸附性能良好的活性炭。试验采用Plackett—Burman（PB）实验设计和Box—BehnkenDesign（BBD）设计法对影响活性炭碘吸附值的6个条件进行筛选优化。PB实验设计与统计学分析表明酸屑比、研磨时间、活化温度、磷酸浓度是影响活性炭碘吸附值的四个关键因素。以碘吸附值为响应目标,对四因素进行BBD设计,并经响应面法优化分析得到影响活性炭碘吸附值的二阶模型,确定了机械力化学技术制备磷酸活性炭的较优操作条件为：酸屑比2．00,研磨时间22min,活化温度406℃,磷酸浓度20％,活性炭的碘吸附值达1195．23mg／g。     

响应面法优化软包装风味鱼的加工工艺

采用响应面法优化了风味鱼的加工工艺参数.通过对盐渍时间、盐渍浓度、卤制时间以及杀菌温度和时间的研究,确定风味鱼块的加工方法为：新鲜鱼切块后,采用4%的食盐在常压下盐渍3.8h或真空下盐渍100min,清洗沥干,再用一定比例的调味液卤制89min,沥干后送入鼓风干燥箱中进行阶段干燥（温度为30℃,干燥30min,升温至50℃干燥30min,最后升至100℃干燥2h）,取出冷却后进行真空包装,在115℃-118℃的条件下杀菌20min,得到的软包装风味鱼具有外观好、香味浓郁、耐咀嚼等特点.