免疫细胞分离鉴定综合实验设计

在传统免疫细胞分离检测实验教学的基础上,利用高性能正置荧光显微镜和流式细胞仪以不同技术检测鉴定免疫细胞,对实验教学内容和教学方法进行了改革和实践.将教学与临床科研相结合,利用大型贵重仪器以及合理安排实验时间开发综合实验.结果表明,学生不仅可以更有效消化医学免疫学课程基础知识,了解学科新技术的发展,而且可以培养学生的科研...     

肌乳酸跨膜运输

乳酸生成后的代谢去向主要是氧化清除.乳酸可以生成部位通过组织间隙和血液穿梭到邻近的高氧化能力部位.乳酸跨膜运输是在一元羧化乳酸运输蛋白(MCT)协助下沿浓度梯度和PH进行的.通过训练,骨胳肌和心肌细胞的MCT1含量会增加,结果摄取乳酸增加,乳酸生成后从骨胳肌外流增加.更重要的是,线粒体膜也含有MCT,这有助于乳酸在此进行氧化.     

GPS操作模拟器的数模设计与编程

对ＧＰＳ操作模拟器所采用的卫星运行和船舶航行中的船位、定位选星等几个主要数模的建立、编程框图和编程中应注意的问题进行了介绍。该数模已用在ＧＬ—１型ＧＰＳ操作模拟器样机上；模拟效果良好。     

FTIR Measurement of Cellulose Microfibril Angle in Historic Scots Pine Wood and Its Use to Detect Fungal Decay

Microfibril angle (MFA) – the orientation of cellulose fibres in the S2 layer of the secondary cell wall – is a key determinant of the stiffness and strength of timber. The microfibril angle depends on the way in which the timber was grown and its position within the tree. Microfibril angle can be measured by X-ray diffraction and other methods, but the methods in current use are slow or require advanced instrumentation. The aim of this study was to explore the use of polarised Fourier transform infrared (FTIR) microscopy as a relatively fast and inexpensive method for measuring MFA in historic Scots pine (Pinus sylvestris L.). The FTIR measurements were calibrated against X-ray measurements of MFA in modern Scots pine. We observed a wide range in MFA values and a radial pattern of MFA similar to modern Scots pine in undecayed Scots pine heartwood from sixteenth and seventeenth century beams in Scottish secular buildings. The density of the heartwood was also similar to modern plantation-grown Scots pine despite the much slower growth rate recorded in the ring widths of the historic timber. The sapwood, which had been attacked by both insect pests and fungi, showed an erratic reduction in density and a large increase in MFA compared to the modern material. The increased sapwood MFA was attributed to selective destruction of the S2 layer of the wood cell walls by fungal decay. Using MFA measurements in conjunction with density offers the possibility to estimate the mechanical properties of sound historic pine timber, to detect fungal decay more sensitively than by density alone, and to distinguish between pest and fungal attack in a way that relates directly to the remaining mechanical performance of the timber.     

全国文化信息资源共享工程卫星数据广播系统终端应用模式探析

随着卫星建设的不断推进,现有卫星终端在应用过程中显现了一些问题:卫星传输的资源与个性化服务需求之间存在一定差距,终端操作复杂,建设及运行维护成本较高。为了改进卫星终端服务模式存在的弊端,增强卫星终端设备及软件的易用性,实现定制化资源传输,应采用双向卫星通信及数据接收机顶盒技术,以改进当前卫星终端应用模式,进一步提高信息服务水平。     

自动检测及连续点火的燃气控制电路

燃气灶具在燃烧过程中，因某种原因，意外熄火的情况时有发生，如果不及时关闭燃气通路或者重新点火燃烧，就会导致严重后果。针对重新点火燃烧的情况，设计出一种具有自动检测熄火及连续点火功能的安全型燃气灶具控制电路是可行的。它主要由电磁阀控制电路、熄火检测电路、点火控制电路及脉冲点火电路等组成，由二节电池供电。理论分析表明，这种安全型燃气灶具工作可靠、结构简单、适用。     

Coulter HMX全自动血细胞分析仪的使用评价

[目的]了解Coulter HMX全自动五分类血细胞分析仪的主要性能,并对该仪器的精密度、准确性、携带污染率、线性、相关性等进行评价.[方法]使用新鲜静脉抗凝血及质控品,对主要参数和白细胞五分类、网织红细胞计数进行双盲法测定.[结果]该仪器精密度高,结果准确可靠,线性良好.携带污染率WBC、RBC、HGB、PLT均小于2％.网织红细胞计数与手工法相关性良好,r为0.912.白细胞分类结果与人工镜检结果相比,中性粒细胞、淋巴细胞、嗜酸性粒细胞相关性良好,而单核细胞、嗜碱性粒细胞相关性不理想.[结论] Coulter HMX血细胞分析仪是适用于大、中型医院常规检测的具有良好性能的仪器.     

温敏不育水稻1356S花粉的电镜观察

扫描电镜观察表明13565不育花粉外形多凹陷成毡状或其它不规则形状,而可育花粉外形为圆球形。1356s不同育性花粉的亚显微结构观察比较显示可育花粉细胞质浓厚,细胞器丰富,在花粉充实期已开始形成大空的淀粉粒;而不育花粉在单核靠边期时就已停止发育,随后内质网,核糖体等细胞器解体,线粒体结构异常,细胞核核质解体。1材料与方法1．工材料：高温敏感不育水稻135651．2方法：互．21扫描电镜观察材料的制备“‘取1356S不同育性时期发育成熟的花药,经2．5％戊二醇固定,用丙酮异戊酯法脱水,临界点干燥法（HCP－2机）干燥。将干燥后的…