SnCl_2溶液的配制及反常现象的探讨

本文探讨了S_nCl_2溶液的配制方法和配制过程中所出现的问题及S_nCl_2溶液性质的一些反常现象。     

苯甲酸分子的简正振动与拉曼光谱

利用量子化学软件计算得出了苯甲酸分子的平衡构形,基于这个构形,理论上计算出了苯甲酸分子的简正振动光谱,并与实验测得的结果进行了比较,最后对其简正振动模式进行了全面归属.     

乙二胺四乙酸存在下碳酸钙的溶剂热合成

在乙二胺四乙酸（EDTA）存在下,分别在超纯水、i—C3H8OH和CH,OH溶剂中,120℃温度下水热法合成了CaCO3晶体,用粉末XRD衍射方法和红外光谱对合成的晶体进行了表征,用扫描电子显微镜观察分析了生成的CaCO3晶体的形貌,研究溶剂对CaCO3晶体的晶型和形貌的影响．结果发现,在纯水中和i—C3H8OH得到主要是文石和少量球霰石晶体,但晶体形状不同;而在CH3OH溶剂中得到的是方解石晶体,结果说明溶剂对晶型和形状均有影响．     

生物大分子的共振光散射分析

文章介绍了共振光散射技术及其在生物大分子定量分析中的应用,并讨论了其发展方向和前景。     

Yangxueqingnao particles inhibit rat vascular smooth muscle cell proliferation induced by lysophosphatidic acid

Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). Methods: The amount of3H-TdR (3H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation end product malondialdehyde (MDA)content of the VSMC were assayed. Results: 1×10-9, 1×10-8, 1×10-7 mol/L LPA in a concentration dependent manner, induced the amount of 3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%,and 15% Yangxueqingnao serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P＜0.01) respectively in the amount of 3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P＜0.05), 29.6% (P＜0.01), and 48.9% (P＜0.01)respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P＜0.01) respectively, in the 1×10-7mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.     

陆英中乌索酸的分离制备及HPLC分析(Ⅲ)

目的：分离制备陆英中的乌索酸，并建立其含量测定方法．方法：采用“醇提凝析法”分离制备乌索酸，HPLC法测定其含量，色谱柱为Symmetry Shield RP18，流动相为甲醇-水(88：12)，流速1ml／min，检测波长220nm，柱温25℃．结果：乌索酸在4．6～23．0μg范围内线性关系良好(r=0．9999)，平均回收率为99．82％(RSD为0．75％)．结论：“醇提凝析法”分离制备乌索酸工艺先进，实用可行；HPLC法测定乌索酸含量准确、可靠，可用于乌索酸含量测定分析。     

氢键对有机物性质和结构的影响

探讨了氢键的本质、种类及其对有机物结构和性质的影响。     

Chelators effect on soil Cu extractability and uptake byElsholtzia splendens

Phytoremediation is emerging as a potential cost-effective solution for remediation of contaminated soils, and bioavailability of metal in the soil for plant uptake is an important factor for successful phytoremediation. This study aimed at investigating the ability of EDTA and citric acid for enhancing soil bioavailability of Cu and phytoremediation by Elsholtzia splendens in two types of soils contaminated with heavy metals [i.e. mined soil from copper mining area (MS), and paddy soil (PS) polluted by copper refining]. The results showed that addition of 2.5 mmol/kg EDTA significantly increased the H₂O extractable Cu concentration from 1.20 to 15.78 mg/kg in MS and from 0.26 to 15.72 mg/kg in PS, and that shoot Cu concentration increased 4-fold and 8-fold as compared to the control. There was no significant difference between the treatment with 5.0 mmol/kg EDTA and that with 2.5 mmol/kg EDTA, probably because that 2.5 mmol/kg EDTA was enough for elevating Cu bioavailability to the maximum level. As compared with the control, citric acid had no marked effect on both soil extractable Cu and shoot Cu concentration or accumulation. The results indicated that EDTA addition can increase the potential and efficiency of Cu phytoextraction byE. splendens in polluted soils. Project supported by the National Natural Science Foundation of China (No. 29977017) and the Science and Technology Ministry of China (No. 2002CB410804)     

丁酸异戊酯合成中的催化剂比较研究

本文进行了硫酸、对甲苯磺酸、氨基磺酸、强酸性阳离子交换树脂、六水三氯化铁、二水二氯化锡、十八水硫酸铝、十二水合硫酸铁铵、一水硫酸氢钠、固体超强酸、杂多酸和沸石分子筛等催化剂催化合成丁酸异戊酯的比较研究。     

Clinical usefulness of alterations in sialic acid,sialyl transferase and sialoproteins in breast cancer

Sialic acid, the end moieties of the carbohydrate chains are biologically important and essential for functions of glycoconjugates and are reported to be altered in cancer patients. Two hundred and twenty five breast cancer (BC) patients, 100 patients with benign breast disease (BBD) and 100 healthy females (controls) were enrolled for the study. Eight hundred and twenty four follow-up samples of 225 breast carcinoma patients were also evaluated. The association of sialic acid forms, sialyltransferase and α-2–6 sialoproteins levels with presence and extent as well as prognosis of breast carcinoma was studied. Serum sialic acid forms and sialyltransferase revealed significantly elevated levels among untreated breast cancer patients as compared to the controls, patients with BBD as well as cancer patients in remission. Non-responders showed comparable levels of the markers with those found in breast cancer patients at the time of diagnosis. Higher levels of sialic acid forms at diagnosis were associated with poor prognosis. A positive correlation between serum levels of different forms of sialic acids and extent of malignant disease was observed. The changes in serum proteins with terminal α-2–6 sialic acid correlated well with alterations in the levels of sialic acid forms and sialyltransferase. Malignant tissues showed elevated levels of sialic acid and sialyltransferase as compared to surrounding normal tissues. The results suggested potential utility of these markers in evaluation of clinical outcome.