试析隋李谔《上书正文体》

东晋以降,文风浮华,内容空洞,“损本逐末”。隋初,治书侍御史李谔提请文帝杨坚用行政手段端正公文文。本文以其《上书正文体》为研究对象,从文种学角度出发,探索“上书”的历史渊源与向“奏”文种转化;分析后世沿用残留状态的上书之原因,印证该实例处的文种演化阶段。从文体写作的角度出发：分析该文所崇尚的“儒素”之内涵,是强调公文以实用为本。“儒”指内容实在,“素”之指文字朴实。与文艺作品基本出发点,以意境为统率,情趣为根本,欣赏为目的,明显有异。进而分析李谔的主张在公文写作指导思想上的局限性。     

Homoharringtonine induces apoptosis of endothelium and down-regulates VEGF expression of K562 cells

Homoharringtonine (HHT) has currently been used successfully in the treatment of acute and chronic myeloid leukemias and has been shown to induce apoptosis of different types of leukemic cells in vitro. Emerging evidence suggests that angiogenesis may play an important role in hematological malignancies, such as leukemia. How ever, whether HHT can relieve leukemia by anti-angiogenesis is still unknown. We investigated the anti-angiogenesis potential of HHT with the human umbilical vein endothelial cell line (ECV304) and leukemic cell line (K562) in vitro. Cellular proliferation was determined by MTT assay and apoptosis was analyzed by flow cytometry. The mRNA expression of vascular endothelial growth factor (VEGF) was assessed by RT-PCR and VEGF protein production was detected by Western blot. Inhibition of cell proliferation and induction of apoptosis by HHT were discovered in ECV304 cells, and appeared in a dose- and time-dependent manner. Also, treatment with HHT caused down-regulation of VEGF mRNA expression in K562 cells in similar dose- and time-dependent manner and inhibition of VEGF protein production in K562 cells in response to the enhancing concentration of HHT. The results demonstrated that HHT could also induce apoptosis in endothelium and down-regulate VEGF expression in K562 cells. In conclusion, we believe HHT has anti-angiogenesis potential and speculate that HHT might exert its anti-leukemia effects via reduction of angiogenesis.     

Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…     

自聚焦克尔类电介质中非傍轴光束调制非稳的研究

Propagation stability of non-paraxial beam in nonlinear Kerr media is investigated with a linear stability method.Both theoretical analysis and numerical simulation show that modulation instability(MI) gain spectrum has three different distribution features determined by the times of incident power P0 and the non-paraxial parameter a. Furthermore,the corresponding criterion is put forward to distinguish the three different distributions.     

关于粘性导数的几个性质的探讨

M .G .Crandall等人在文 [1]中给出了函数的粘性导数的概念。在这个概念的基础上我们讨论了函数的粘性导数的上 (下 )导集的几个性质 :在函数可微点处的性质、闭凸性质、与C1类函数的和的性质、与C2 类函数的差在严格局部极大 (小 )值点的性质。     

拒绝服务攻击原理与防御策略

介绍了拒绝服务攻击实施的原理，以及相关协议的漏洞和拒绝服务攻击的最新进展，并说明了如何结合防火墙和入侵检测系统等手段来防范其攻击。     

论21世纪图书馆的发展模式与功能

本文分析了影响决定图书馆发展模式的四个重要因素。即技术、资金、文化心态和制度变迁。认为模式的不同导致图书馆功能在不同时代的差异，并提出了21世纪图书馆的四种发展模式，阐述了它们的主要功能。     

一类超二次Hamilton系统的周期解的存在性

运用临界点理论中的极小、极大方法得到一类超二次哈密顿系统的周期解的存在性的存在性定理.     

Physical design method of MPSoC

Floorplan, clock network and power plan are crucial steps in deep sub-micron system-on-chip design. A novel di- agonal floorplan is integrated to enhance the data sharing between different cores in system-on-chip. Custom clock network con- taining hand-adjusted buffers and variable routing rules is constructed to realize balanced synchronization. Effective power plan considering both IR drop and electromigration achieves high utilization and maintains power integrity in our MediaSoC. Using such methods, deep sub-micron design challenges are managed under a fast prototyping methodology, which greatly shortens the design cycle.     

长光程薄层光谱电化学技术及应用

提供电子转移反应实验研究的实验装置及技术，依此可获得电子转移数、式电极电势、式异相电子转移速率常数、电子传递系数及还原态和氧化态的扩散系数等一系列重要的电化学参数。还可依此为基础设计出新颖、实用的教学实验。