Analysis of both chitinase and chitosanase produced by <Emphasis Type="Italic">Sphingomonas</Emphasis> sp. CJ-5

A novel chitinolytic and chitosanolytic bacterium, Sphingomonas sp. CJ-5, has been isolated and characterized. It secretes both chitinase and chitosanase into surrounding medium in response to chitin or chitosan induction. To characterize the enzymes, both chitinase and chitosanase were purified by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-Sepharose Fast Flow. SDS-PAGE analysis demonstrated molecular masses of chitinase and chitosanase were 230 kDa and 45 kDa respectively. The optimum hydrolysis conditions for chitinase were about pH 7.0 and 36 °C, and these for chitosanase were pH 6.5 and 56 °C, respectively. Both enzymes were quite stable up to 45 °C for one hour at pH 5~8. These results show that CJ-5 may have potential for industrial application particularly in recycling of chitin wastes.     

用离子液体合成含氟1,3,4-噻二唑方法的探索研究

制备一种新型BrФnsted酸性离子液体[Hmim]HSO4为催化剂,来合成1,3,4-噻二唑类杂环化合物,通过简单的减压蒸馏可以实现催化剂和产物的分离。比较此方法与传统合成方法产率的不同,确定其最佳反应条件以及探讨该离子液体的重复使用性能。实验结果表明,未优化1,3,4-噻二唑的产率为68％,相对于传统的合成工艺,提高了10％（传统的合成产率为57．6％）;该反应的最佳反应时间为3h;离子液体循环使用3次,1,3,4-噻二唑类杂环化合物的产率均大于63％。     

常深度线性水波的调制

从线性水波方程出发,采用多重尺度法讨论了常深度水波的调制问题,严格推导出了控制包络波幅的缓慢调制方程满足线性Schr(?)dinger方程的结论．     

磁场处理在诺卡氏菌诱变选育中作用的研究

诺卡氏菌HD9611经紫外线诱变处理后在磁场条件下进行培养，其诱变致死率和营养缺陷的诱发率均比诱变后在自然条件下培养的要高，并且经UV＋磁场复合诱变所筛选出的菌株酶活也单纯紫外线处理的时照菌株平均酶活相对提高了17．2％。     

Direct transformation of n-alkane into all-trans conjugated polyene via cascade dehydrogenation

Selective C(sp³) −H activation is of fundamental importance in processing alkane feedstocks to produce high-value-added chemical products. By virtue of an on-surface synthesis strategy, we report selective cascade dehydrogenation of n-alkane molecules under surface constraints, which yields monodispersed all-trans conjugated polyenes with unprecedented length controllability. We are also able to demonstrate the generality of this concept for alkyl-substituted molecules with programmable lengths and diverse functionalities, and more importantly its promising potential in molecular wiring.     

木霉菌防治油菜菌核病和辣椒白绢病的研究

本文报道了一种生物保护剂──本霉菌防治抽菜核病和辣椒白绢病的途径及效果．     

温度、pH及碳氮源对产色素青霉菌株（Penicilliumsp．）生长的影响

PSO312是从土壤分离的1个青霉菌株,生长中能产生不同酸碱性条件下显黄色与紫红色等天然色素物质．通过菌株固体平板上恒温培养,168h测定菌落直径的方法研究了温度、pH及碳、氮源对菌株（Penicilliumsp．）生长的影响．研究表明31℃为菌株生长的最适温度．31℃培养以pH2—3的最快,pH8—9生长次之,24℃培养以pH8—9生长最快,pH3—4其次．菌株以蛋白胨4g／L、牛肉膏4g／L、酵母膏2g／L及尿素2g／L为氮源培养的菌落直径分别为55．75mm、51．5mm、55．5mm及25．3mm．在（NH4）2SO4 2g／L、NH4NO3 3g／L、NaNO3 4g／L、KN034g／L用量之上培养生长最快,菌落直径分别为35mm、34mm、35mm、及32mm．葡萄糖、蔗糖用量30g／L、果糖20g／L及乳糖25g／L的生长速率最快,菌落直径分别为45mm、38mm、38mm及39mm．丙三醇与淀粉25g／L培养的菌落直径均为39mm．     

脂肪酸延长酶和脱饱和酶在酿酒酵母中的共表达

将一种同时含C20-Δ5脂肪酸碳链延长酶（TFD5）基因和C22-Δ4脂肪酸碳链脱饱和酶（FAD4）基因的双基因共表达质粒转化于Saccharomyces cerevisiae中,形成了工程菌株SC.PYFTD5-FAD4.并对工程菌株SC.PYFTD5-FAD4进行了诱导表达,可直接转化底物二十碳五烯酸（EPA,20：5△5,8,11,14,17n-3）生成二十二碳五烯酸（DPA,22：5△4,7,10,13,16n-3）和二十二碳六烯酸（DHA,22：6△4,7,10,13,16,19n-3）.     

Analysis of endophytic fungi in roots of Santalum album Linn. and its host plant Kuhnia rosmarinifolia Vent.Si-sheng SUN,Xiao-mei CHEN,Shun-xing GUO简

Santalum album Linn. is an evergreen and hemi-parasitic tree, the heartwood-sandalwood of which was used during a long history in traditional Chinese medicine. Kuhnia rosmarinifolia Vent. is a good host for 1- or 2-year-old growing S. album. The interaction between S. album and K. rosmarinifolia is still little known. Many studies have been carried out on a number of plants for identification and diversity of endophytes. In this study, in total 25 taxa of endophytic fungi were isolated from the roots of S. album and the roots of K. rosmarinifolia. The most frequently isolated genera were Penicillium sp. 1 and Fusarium sp. 1 in the roots of S. album and K. rosmarinifolia, respectively. S. album is a root parasite of K. rosmarinifolia. The interesting result is that they apparently do not share the same endophytic fungi isolates. This study for the first time explored the content of endophytic fungi from S. album and K. rosmarinifolia, which provides important information for further studies.