以企业竞争优势为中心的3M经营成果测定法

在日益复杂化的市场竞争环境中,影响企业经营成果水平的因素越来越多。使用何种方法对企业经营成果进行测定才更为合理准确,一直是广大学者和企业战略家们探讨的热门话题。本文分析了传统企业成果测定方法的局限性,从战略角度出发,以企业竞争优势为中心,介绍了3M经营成果测定法;剖析了其与企业竞争优势之间的关系,并设定了3M经营成果测定模型。希望能够为以Concordance Process理论为代表的关于企业竞争优势的各种研究提供一种准确可行的成果测定方法,使今后的实证研究更加严谨科学。     

反相高效液相色谱法测定烟碱中间体制品的含量

本文建立了一种以pH=8.5醋酸-氨溶液作为缓冲溶液,梯度洗脱测定了高含量烟碱制品中烟碱含量的方法。该方法分离效果好、灵敏、准确,可用于烟碱制品的产品质量控制。     

用高度特异性王浆主蛋白1多克隆抗体ELISA法检测蜂王浆新鲜度(英文)

目的:为蜂王浆主蛋白1(MRJP1)的快速检测和鉴别提供科学依据,为蜂王浆的质量控制提供技术支持。创新点:首次比较了MRJP1特异性多克隆抗体与MRJP1重组表达蛋白多克隆抗体对王浆主蛋白家族的免疫反应差异,验证了蜂王浆中MRJP1蛋白降解与保温时间的相关性,建立了以MRJP1作为蜂王浆新鲜度生物标志物的快速检测方法。方法:通过蜂王浆主蛋白家族蛋白的氨基酸序列同源性分析,筛选出MRJP1的特异性多肽区域,进行人工合成,免疫兔子后取血清制备成特异性多克隆抗体。用蛋白质印迹法(Western blot)检测了MRJP1特异性多克隆抗体与MRJP1重组表达蛋白多克隆抗体对王浆主蛋白家族的免疫反应。以新鲜蜂王浆为对照品,用MRJP1特异性抗体酶联接免疫吸附剂测定(ELISA)法和变性电泳胶灰度扫描法分别测定保温(40°C)7~49天的蜂王浆中MRJP1含量的变化,并进行了相关性分析。结论:MRJP1的特异性抗体对MRJP1蛋白具有专一的免疫识别特性,可特异性地检测代表蜂王浆新鲜度的MRJP1含量变化,并鉴别蜂王浆的真伪。     

免疫放射分析法与浓缩接种法检测稻种带细菌性条斑病病菌的比较研究

浓缩接种法是传统的种子带菌检测方法,对水稻细菌性条斑病菌的可检测浓度为10~5个/ml左右,检测一批样品的速度为7～10d,但由于受到诸多环境因素和寄主的影响,该方法的稳定性和准确性较差。免疫放射分析法是一种新颖的超微量检测技术,由于它具有放射性同位素的灵敏性和血清学的特异性,用于细菌性条斑病菌的检测与前者相比具有许多优点,灵敏度高,可检测浓度为10~3个/ml;速度快,4h左右可得出结果;稳定性好,抗血清冻干品在4℃和-25℃下分别可保存70d和300d;可靠性强,与浓缩接种法的阴性符合率为100％,阳性符合率为63.04％。特别在低浓度下检测准确性更高。     

商洛茶叶中痕量锰催化光度法测定

茶叶中含有痕量锰,采用Mn(Ⅱ)—甲酚红—氨三乙酸—NH3.H2O—KIO4体系进行催化氧化光度法测定,灵敏度可达6.1×10-10g/mL,选择性高,常见的二十余种阴、阳离子均无干扰,结果重现性好.结果表明,商洛出产的几种茶叶含锰量均略高于被测试的几种外地茶叶.     

催化动力学光度法测定痕量钛

在HCl-NaAc(pH=0.90)酸性介质中,以钛催化过氧化氢氧化苯基荧光酮为指示反应,建立了一种催化动力学光度法测定痕量钛的新方法,并尝试用于测定大米、头发中的痕量钛(Ⅳ)。结果表明,在实验确定的最佳条件下,该方法的最低检出限为2.61×10-6 g/L,相对标准偏差为2.05%,测定的线性范围为(0.01～0.08)mg/L,结果令人满意。     

Enantioselective assay of S( )- and R(-)-propafenone in human urine by using RP-HPLC with pre-column chiral derivatization

INTRODUCTION Pharmacokinetic and pharmacodynamic dif-ferences involving stereoselective drug disposition are important for clinical evaluation of some chiral compounds that are rountinely administered in their racemic form (Zhou et al., 2002). Propafenone (PPF), a kind of Ic antiarrhythmics drug, is now marketed as racemate. The two enantiomers of PPF are equi- potent in terms of sodium channel-blocking activity, but the main side effect is the b-adreno recep-tor-blocking action of t…     

Science Letters：Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method

A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action （A0） was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance （Ab） was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from AA, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV〈4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine 〈0.32 mmol/L in serum had no significant effects. △A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.     

萌发花生种子中CaM含量变化与种子活力

应用ELISA测定了萌发花生种子中CaM的含量,种子的大部分CaM存在于胚轴中,小部分存在于子叶。高活力种子CaM含量较高,在吸胀48h出现一个特征峰,随种子活力下降,CaM含量也减少,其特征峰延迟至 72h才出现,且峰值也低,Ca~(++)可提高种子活力,也提高CaM的含量,使特征峰从72h提早到48h出现,TFP使种子CaM含量下降,直至萌发96h仍来山现特征峰,Ca~(++)、CaM与种子活力密切相关。     

Molecular Studies on Coronary Artery Disease—A Review

Coronary artery disease (CAD) remains the major cause of mortality and morbidity in the entire world population. The conventional risk factors of CAD include hypertension, hyperlipidemia, diabetes mellitus, family history, smoking etc. These factors contribute only 50 % of the total risk of CAD. For providing a complete risk assessment in CAD, it is mandatory to have well-planned clinical, biochemical and genetic studies in patients with CAD and subjects who are at risk of developing CAD. In this review an attempt is made to critically evaluate the conventional and emerging risk factors which predispose the individual to CAD. Specifically, the molecular basis of CAD including high oxidative stress, low antioxidant status and increased DNA damage are covered. A comprehensive and multifactorial approach to the problem is the better way to reduce the morbidity and mortality of the disease.