Determination of Biological Variance and Validation of a Fluorometric Assay for Measurement of α-l-Iduronidase Activity in Dried Blood Spots Samples: The First Experience in Iran

Methods for assaying lysosomal diseases in dried blood samples are very useful today due to its several advantages related to the stability of samples, its transportation, handled and analysis, and its potential use for newborn screening compared to traditional methods in leucocytes samples. For this reason, it is important to validate these assays before being used in routine laboratory. Because of different in biological markers based on ethnicity, we aimed this study to validation a DBS-based fluorometric assay for measurement of α-l-Iduronidase activity for diagnosis of MPS I patients in Iran. DBS samples were collected from 15 MPS I patients and 60 healthy age matched subjects. Diagnostic value, biological variance and α-l-Iduronidase activity were determined. DBS α-l-Iduronidase activity was significantly higher in male subjects than in female group. Using a cut-off level of 1.08 µmol/spot 20 h, sensitivity and specificity were 100 and 98 %. The linearity of test was proved and we showed that within-run and between run precision were 5.6 and 14.66 %. Measurement of α-l-Iduronidase activity in DBS samples is an accurate test for diagnosis of MPS I and because of its rapid shipping and simplicity to keeping, DBS-based enzyme activity could be considered as a useful diagnostic tool in this disease.     

Age-Related Changes in Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1) and Receptor 2 (sVEGFR2) in Healthy Japanese Subjects

We measured serum soluble vascular endothelial growth factor receptor 1 (sVEGFR1) and receptor 2 (sVEGFR2) levels in healthy Japanese individuals in order to establish a reference value using a specific ELISA. Significant differences were observed in serum sVEGFR1 and sVEGFR2 levels between children and adults. To demonstrate the usefulness of the reference value for children, we measured serum sVEGFR1 and sVEGFR2 levels in children with diarrhea positive (D+) hemolytic uremic syndrome (HUS) as a preliminary study. Serum sVEGFR2 levels in children with HUS were markedly higher than those in healthy children from the onset of D + HUS. The reference value for healthy children in the present study will allow normal and pathological conditions to be discriminated from each other in future study     

信息共享空间研究的知识图谱分析

以CSSCI数据库收录的以信息共享空间为主题的论文为基础数据,通过Citespace3.8R1软件对相关引文数据和主题词数据进行处理,绘制出信息共享空间领域的知识图谱,并对其进行分析,得出我国信息共享空间领域的重要学术文献及学术代表人物、主要研究热点及研究前沿等。     

AR阻断剂对运动骨骼肌ERK1/2信号通路的影响

目的:通过阻断AR,以探讨雄激素对运动骨骼肌ERK1/2通路的影响特点。方法:7周龄雄性SD大鼠30只,随机分为对照组、AR阻断剂组、运动组、AR阻断剂运动组、假手术组。AR阻断剂组于颈部皮下包埋氟他胺释缓剂,运动组进行10 d中等强度运动,于末次运动后6 h取趾长伸肌进行指标测定。结果:与对照组相比,AR阻断剂组趾长伸肌湿重、肌纤维横截面积、AR mRNA及p-ARSer210均显著下降(p<0.01-0.05),MEK1/2、ERK1/2、P90RSK的mRNA及蛋白磷酸化水平未有显著变化。运动组AR及ERK1/2通路的mRNA及蛋白磷酸化水平则显著增加(p<0.05),而其趾长伸肌湿重、肌纤维横截面积并未有显著变化。AR阻断剂运动组各指标与运动组相比均显著下降(p<0.01-0.05),而与AR阻断剂组相比均未有显著变化。结论:阻断AR可显著抑制运动骨骼肌湿重、横截面积及AR的基因和磷酸化表达,证实雄激素对运动骨骼肌的促蛋白合成作用主要经AR介导;AR阻断剂可显著抑制运动骨骼肌ERK1/2通路激活,提示雄激素经AR介导发挥非基因作用。该研究对雄激素促进运动骨骼肌蛋白合成的非基因作用提供一定实验依据。     

基于MATLAB的无约束优化问题对称秩-1法与BFGS法之探讨

对称秩-1法和BFGS法是用拟牛顿法求解无约束优化问题时最常见的两种方法,它们都具有计算简单、收敛速度快等优点.探讨两种方法的算法格式、收敛速度和计算精度问题,同时利用MATLAB软件编程进行实例求解.结果表明:在解的迭代次数和精确度方面,BFGS算法均明显优于对称秩-1法.     

“1号店”安阳分店网络顾客忠诚度分析

在当今社会,网络技术的不断突破,客户结构的传统商业模式发生了巨大变化,顾客的忠诚度越来越难以支撑。因此,本文以“1号店”为例,在电子商务环境下对顾客的忠诚度进行一个全面的研究,通过对“1号店”顾客忠诚度的研究,更有效地提出提高顾客忠诚度的措施,使企业拥有一批忠诚的客户资源,从而使得企业的长期利益得到更好的保证,使企业在未来竞争中处于一个有利的位置,也希望能为其他企业起到借鉴的作用。     

CRP,but not TNF-α or IL-6, decreases after weight loss in patients with morbid obesity exposed to intensive weight reduction and balneological treatment

Objective

The aim of this study was to evaluate the concentrations of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and the degree of homeostasis model assessment-insulin resistance (HOMA-IR) in patients with morbid obesity exposed to a three-week low-calorie diet and balneotherapy.

Methods

The study included 33 patients (25 females and 8 males; mean age 46 years) with body mass index (BMI) values of >40 kg/m². Evaluations of CRP, IL-6, TNF-α, lipid profile, HOMA-IR, and fasting glucose were carried out before (baseline data) and three weeks after the treatment. The control group consisted of 20 healthy volunteers (15 females and 5 males) with a mean age of 39 years and BMI values of ≤24.9 kg/m².

Results

In the blood of patients with morbid obesity we found significantly elevated levels of CRP, TNF-α, triglycerides, HOMA-IR and fasting glucose, but a decreased level of high density lipoprotein (HDL)-cholesterol, compared with the healthy individuals. The treatment resulted in about a 9.4% reduction in body weight from 122.5 to 111.0 kg and a significant decrease in the concentration of CRP, but no change in TNF-α or IL-6. HOMA-IR was significantly reduced.

Conclusion

The decrease in CRP level without changes in TNF-α or IL-6 concentrations after the low-calorie diet and balneological treatment, suggests that an essential amount of adipose tissue must be removed before proper adipocyte function is restored. The decrease in HOMA-IR indicates an improvement in insulin sensitivity, which is beneficial in obese patients.

     

况周颐《存悔词》足本、删定本考论

《存悔词》是况周颐刊印的第一本词集。从1879年至1925年,况周颐不断对其删、增、改、定,故传世的《存悔词》有足本、删定本之分。由于其滞后的文献整理,目前学术界仅参引其删定本。《存悔词》计有足本2种、删定本1种,收录词集自序2篇、词作69首。这些不同版本的《存悔词》,有利于我们深入了解况周颐的词创作、词学理论的演变。《存悔词》所录词作抒发性灵,轻倩流慧,具有一定文学价值;同时,它为研究况周颐及其词学、近代文学提供宝贵资料,具有重要的文献价值;此外,词集自序及删改过程,蕴含丰富的词学理论,具有较高的学术价值。     

具有复介电常量对称结构一维三元光子晶体透射谱的研究

利用传输矩阵法理论,研究含复介电常量对称结构一维三元光子晶体的光传输特性。结果表明:当各层介质的介电常量均为实数时,在较宽的禁带范围内出现一条透射率为100%的透射峰;当介质介电常量含正虚部时,禁带中的透射峰出现透射衰减现象,若含有负虚部时,透射峰则出现透射增益现象;随着复介电正虚部的增大,透射峰出现单调衰减,而随着复介电负虚部绝对值的增大,透射增益达到一极大值,随后减小;在不同介质层引入复介电常量引起透射峰的透射率衰减或增益强度不同。这些特性对设计光放大器、衰减器等新型光学器件有一定的参考价值。     

基于多变量优化MGM(1,n)模型的郑州市用水量预测

用水量是城市发展水平的重要评价指数。鉴于用水量系统复杂性和非线性的特征,结合郑州市居民用水量的历史数据,通过建立优化背景值的MGM(1,n)模型,实例预测郑州市2008至2015年用水量需求并指出城市居民用水量发展趋势。