DNA分子标记技术在水生动物遗传多样性研究中的应用

简要阐述了几种常见DNA分子标记技术,如限制性片断长度多态性(restriction fragment length polymorphism,RFLP)、随机扩增多态性DNA(random amplified polymorphie DNA,RAPD)、扩增片断长度多态性(amplified fragment length polymorphism,AFLP)、简单重复序列或微卫星标记(simpie sequencerepeat,SSR)、内部简单重复序列(inter-simple sequence repeat,ISSR)和单核苷酸多态性(single nucleotidepelymorphisms,SNP)等的基本原理、技术上的优缺点及其在水生动物遗传多样性研究中的应用,包括遗传多样性的鉴定、品种亲缘关系与分类的研究和种质资源的鉴定等.最后,对DNA分子标记技术在水生动物遗传多样性应用中的存在问题进行了简单的讨论.     

从"背影"的再现看风格的可译性--读张培基英译《背影》有感

本文以<背影>为例,分析了如何用风格的形式标记和非形式标记认识和再现文章风格的程序,从而证明了风格是可译的这一论断.     

从语用角度看虚拟语气的动词形式

虚拟语气是一种动词形式,表示说话人的意图和态度,包括命令、建议、愿望、假设、委婉、祈求等多重含义。本文从语用角度分析虚拟语气的特点,并进行重新归类。     

The effects of short-term jump training on bone metabolism in females using oral contraceptives

The purpose of this study was to determine the effect of oral contraceptive use on bone serum markers following a 3-week jumping protocol. Twenty-three females (18–25 years) were grouped as oral contraceptive users (OC+) or non-users (OC?). Following a 3-week observation period, participants completed a 3-week (15-day) jump protocol. Jump sessions consisting of ten 42 cm drop jumps with a 30 s rest interval between jumps were completed each day, 5 days per week. Peak vertical ground reaction force and loading rate were measured and the osteogenic index was calculated. Serum markers for bone formation, bone alkaline phosphatase (BAP) and bone resorption, C-terminal telopeptides of type I collagen (CTX) were measured at three time points (pre-, mid-, post-jump). BAP and CTX increased significantly (P = 0.0017, 0.0488) in both groups post-jump; however, bone metabolic markers were not different between the OC+ and OC? groups. Osteogenic index, ground reaction force and vertical jump height were similar between groups. Correlations between markers of bone metabolism and participants’ age at menarche, weight, loading rate and years on OC were not significant. A 3-week jumping protocol was found to be effective in stimulating bone metabolism in both OC+ and OC? groups.     

法语、英语虚拟体系对比研究

以虚拟体系为着眼点,从名称、表达形式、语法功能、句型结构等四个方面,探寻并比较同属印欧语系的法语和英语之间的异同点。通过了解两种语言的相同点,学习者可以利用已有的英语基础去理解相应的法语语法,从而避免差异之处可能对二语习得产生的负面影响。     

Serum markers for early diagnosis of non-‘Q’ wave and ‘Q’ wave myocardial infarction

The present study conducted on twenty-five uncomplicated cases of acute myocardial infarction diagnosed by clinical and electrocardiographic findings indicated significantly increased level of cardiac Troponin-T and increased activities of the enzymes total creatine kinase, creatine kinase-MB, aspartate transaminase and lactate dehydrogenase as compared to the twenty-five healthy control subjects. The level of cardiac Troponin-T and the activities of the enzymes total creatine kinase, creatine kinase-MB, aspartate transaminase and lactate dehydrogenase was found to be higher in “Q” wave myocardial infarction patients as compared to the non-“Q” wave and the controls. Since cardiac Troponin-T has been shown to increase in unstable angina and renal failure without cardiac disease and creatine kinase-MB activity has been found to be normal in patients with unstable angina and increase very slightly in patients with renal failure, it was concluded that a combination of cardiac Troponin-T and creatine kinase-MB activity was sufficient for the early diagnosis of acute myocardial infarction.     

对比性标记语在语言学学术论文段首句中的分布——基于语料库技术

总样本的68.99%分布在句中,30.14%分布于句首,句末鲜见,仅0.87%。当显著性水平接近0.000时,这种差异在统计学上具有显著性。每一标记语在段首句各位置上的选择趋势不等,如but常用于句中、句首,鲜见于句末。句首词频最高的前五个标记语（由高到低）分别有but、although、however、yet和contrast;句中则为but、rather、however、contrast和still;仅有yet、however、at the same time和comparison出现在句末。段首句句中标记语词频大于句首这一统计结果说明,仅以句首位置判定书面语篇标记语是片面的。     

“V来”类话语标记的语篇特征及主观化表现

现代汉语中的"V来"从一个表示"行为义"的词发展为兼表"认知义",后又作"话语标记"的语言结构,经历了一个语法化的过程。同时,也是主观性不断增强的过程。笔者不仅考察"V来"格式的语篇表现情况,探讨其主观化演变的过程,还对"V来"类词进行比较研究,从其残留意义的线索出发辨析其内部主观化的差异。     

江苏省男子羽毛球运动员夏训期间身体机能的生化评定

本文运用多个生化指标对夏训期间江苏省男子羽毛球运动员的身体机能进行了跟踪观察和综合评定。结果表明，训练周期开始尿蛋白、血尿素氮明显升高、后逐渐下降；血红蛋白呈现开始降低后期回升的趋势；而唾液睾酮、皮质醇则处于持续升高水平。提示：运动员对大运动量训练经历了一个从不适应到适应的过程，整个周期中身体机能呈平稳上升趋势。     

Comparison of Teaching Approaches of the Subjunctive Mood—Comparison of Two Grammar Books from Home and Abroad

This study compared the different approaches of introducing the subjunctive mood in Advanced English Grammar and Grammar Dimensions in the hope that it will help ESL teachers to make choice of the proper ways of teaching the subjunctive mood,one of the most serious grammatical problems ever encountered by most ESL learners in China.Also,the main causes of the students’drawbacks in learning subjunctive mood are excavated and some feasible solutions in mastering this difficult item in English grammar are presented in this study.