Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…     

自聚焦克尔类电介质中非傍轴光束调制非稳的研究

Propagation stability of non-paraxial beam in nonlinear Kerr media is investigated with a linear stability method.Both theoretical analysis and numerical simulation show that modulation instability(MI) gain spectrum has three different distribution features determined by the times of incident power P0 and the non-paraxial parameter a. Furthermore,the corresponding criterion is put forward to distinguish the three different distributions.     

关于粘性导数的几个性质的探讨

M .G .Crandall等人在文 [1]中给出了函数的粘性导数的概念。在这个概念的基础上我们讨论了函数的粘性导数的上 (下 )导集的几个性质 :在函数可微点处的性质、闭凸性质、与C1类函数的和的性质、与C2 类函数的差在严格局部极大 (小 )值点的性质。     

拒绝服务攻击原理与防御策略

介绍了拒绝服务攻击实施的原理，以及相关协议的漏洞和拒绝服务攻击的最新进展，并说明了如何结合防火墙和入侵检测系统等手段来防范其攻击。     

论21世纪图书馆的发展模式与功能

本文分析了影响决定图书馆发展模式的四个重要因素。即技术、资金、文化心态和制度变迁。认为模式的不同导致图书馆功能在不同时代的差异，并提出了21世纪图书馆的四种发展模式，阐述了它们的主要功能。     

一类超二次Hamilton系统的周期解的存在性

运用临界点理论中的极小、极大方法得到一类超二次哈密顿系统的周期解的存在性的存在性定理.     

Physical design method of MPSoC

Floorplan, clock network and power plan are crucial steps in deep sub-micron system-on-chip design. A novel di- agonal floorplan is integrated to enhance the data sharing between different cores in system-on-chip. Custom clock network con- taining hand-adjusted buffers and variable routing rules is constructed to realize balanced synchronization. Effective power plan considering both IR drop and electromigration achieves high utilization and maintains power integrity in our MediaSoC. Using such methods, deep sub-micron design challenges are managed under a fast prototyping methodology, which greatly shortens the design cycle.     

长光程薄层光谱电化学技术及应用

提供电子转移反应实验研究的实验装置及技术，依此可获得电子转移数、式电极电势、式异相电子转移速率常数、电子传递系数及还原态和氧化态的扩散系数等一系列重要的电化学参数。还可依此为基础设计出新颖、实用的教学实验。     

课程改革中教师关注阶段理论的研究述评

本文介绍了"关注阶段"的理论架构与评定方法,回顾并分析了它在美国、比利时、荷兰、香港以及澳大利亚等文化情境中的运用与发展.在我国新课程改革背景下进行关注阶段研究不仅有利于我们了解新课程实施情况,而且有助于这一理论自身的发展.     

VaR模型在我国养老基金投资风险控制中的应用

养老基金的投资面临多种风险,要实现其保值增值,必须进行风险度量和监控。VaR模型作为一种新的金融风险管理工具,因其有别于传统的金融风险管理工具的特点,在西方受到广泛青睐。探讨该模型在我国养老基金投资风险控制中的应用。