Protective effect of recombinant Lactobacillus plantarum against H2O2-induced oxidative stress in HUVEC cells

This study probed the protective effect of recombinant Lactobacillus plantarum against hydrogen peroxide(H_2O_2)-induced oxidative stress in human umbilical vein endothelial cells(HUVECs). We constructed a new functional L. plantarum(NC8-p SIP409-alr-angiotensin-converting enzyme inhibitory peptide(ACEIP)) with a double-gene-labeled non-resistant screen as an expression vector. A 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazolium bromide(MTT) colorimetric assay was carried out to determine the cell viability of HUVEC cells following pretreatment with NC8-p SIP409-alr-ACEIP. Flow cytometry(FCM) was used to determine the apoptosis rate of HUVEC cells. Cysteinyl aspartate specific proteinase(caspase)-3/8/9 activity was also assayed and western blotting was used to determine protein expression of B-cell lymphoma 2(Bcl-2), Bcl-2-associated X protein(Bax), inducible nitric oxide synthase(i NOS), nicotinamide adenine dinucleotide phosphate oxidase 2(gp91 phox), angiotensin II(Ang II), and angiotensin-converting enzyme 2(ACE2), as well as corresponding indicators of oxidative stress, such as reactive oxygen species(ROS), mitochondrial membrane potential(MMP), malondialdehyde(MDA),and superoxide dismutase(SOD). NC8-p SIP409-alr-ACEIP attenuated H_2O_2-induced cell death, as determined by the MTT assay. NC8-p SIP409-alr-ACEIP reduced apoptosis of HUVEC cells by FCM. In addition, compared to the positive control, the oxidative stress index of the H_2O_2-induced HUVEC(Hy-HUVEC), which was pretreated by NC8-p SIP409-alr-ACEIP, i NOS,gp91 phox, MDA, and ROS, was decreased obviously; SOD expression level was increased; caspase-3 or-9 was decreased, but caspase-8 did not change; Bcl-2/Bax ratio was increased; permeability changes of mitochondria were inhibited; and loss of transmembrane potential was prevented. Expression of the hypertension-related protein(Ang II protein) in HUVEC cells protected by NC8-p SIP409-alr-ACEIP decreased and expression of ACE2 protein increased. These plantarum results suggested that NC8-p SIP409-alr-ACEIP protects against H_2O_2-induced injury in HUVEC cells. The mechanism for this effect is related to enhancement of antioxidant capacity and apoptosis.     

试析视听媒体语言的雅与俗

媒体语言既有高雅的,也有通俗的,借以适合不同受众的审美需求。雅与俗没有严格的界限,相辅相成,互为依存,在二者之间寻求平衡,雅中孕育着俗,俗中渗透着雅,和谐统一,才能形成雅俗共赏,承担相应的社会责任。文章试图从审视当今媒体语言的现状、分析其原因入手,阐述媒体语言的“雅”、“俗”关系,并对与之相关的一些问题进行论述。     

常曲率空间中具有平行平均曲率向量的伪脐子流形

设(?)为n p q维常曲率空间N~(n p q)中的n p维常曲率子流形,M~n为(?)的n维子流形,设M~n为N~(n p q)中具有平行平均曲率向量的紧致伪脐子流形,本文得到M~n为(?)的全脐子流形的几个Pinching条件.     

Research Summaries for Normal Birth

In this column, the author summarizes research studies relevant to normal birth. The studies summarized include a large trial evaluating the effect of prior vaginal births after a cesarean on outcomes in subsequent births; a study linking umbilical cord blood pH with intellectual outcomes in childhood; and a prospective trial evaluating the effect of routine antenatal nonstress testing on maternal anxiety. The author also highlights four articles about normal birth in a recent nursing journal series dedicated to the topic.     

肺炎衣原体的培养及对人脐带静脉内皮细胞的感染

目的：研究肺炎衣原体的培养及其对人脐带静脉内皮细胞的感染。方法：以人喉表皮癌（HEP－2）细胞培养肺炎衣原体并感染人肺带静脉内皮细胞，光镜及电镜下观察感染情况并计包涵体数。结果：肺炎衣原体能在HEP－2细胞内培养并能感染人肺带静脉内皮细胞，感染后72h，包涵体数最多，96h后仍可见肺炎衣原体在人肺带静脉内皮细胞内存活。结论：肺炎衣原体能感染并存活于人肺带静脉内皮细胞，能导致动脉内膜感染。     

迷向子流形的两个性质

讨论黎曼流形中的迷向子流形,得到了关于迷向子流形的两个定理．     

关于完全有界度量集的极限状态的一个注

证明了一完全有界度量集合在Hausdorff距离之下的极限仍是完全有界集,给出了一个应用本文结果的具体例子。     

C2连续奇异混合插值曲面

利用奇异混合函数将双三次B样条曲面与虚拟曲面片作混合,构造一类可插值给定数据点的插值曲面,该类曲面在边界处可达到C~2连续,参数α可对曲面片整体形状进行调控。进一步,构造了可局部调控形状的带形状参数的插值曲面。     

局部对称空间中的伪脐子流形

讨论了局部对称完备黎曼流形中具有平行平均曲率向量场的紧致伪脐子流形,得到了这类子流形成为全脐子流形及其余维数减小的几个拼挤定理.     

转染瘦素人胎盘源间充质干细胞对经X射线辐照后人脐静脉内皮细胞的成血管潜能和周围炎症的影响

目的:放创复合伤是一种以血管损伤和促炎细胞因子缺乏为特征的难愈性创伤。瘦素(leptin)的直接应用在血管生成和炎症中起着重要作用。本研究构建了一种可持续稳定的leptin表达系统——leptin修饰的人胎盘来源间充质干细胞(HPMSCs/leptin),并探究其对经X射线辐照后的人脐静脉内皮细胞(HUVECs)的成血管潜能及周围炎症的影响和潜在机制。创新点:可持续稳定的leptin表达系统(HPMSCs/leptin)促进受X射线辐照后HUVECs的成血管潜能及外周炎症反应,有助于解决放创复合伤伤口愈合过程中血管损伤和促炎因子缺乏的问题。方法:利用慢病毒载体将leptin基因转染HPMSCs获得HPMSCs/leptin。采用X射线单次照射HUVECs,剂量为20 Gy。细胞迁移侵袭实验技术(Transwell)检测照射后HUVECs的迁移情况。在Transwell体系的基础上,建立HPMSCs与受辐照HUVECs共培养体系。CCK-8比色法测定细胞增殖。酶联免疫吸附法(ELISA)检测促炎细胞因子(粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-1α(IL-1α)、IL-6和IL-8)的分泌。实时荧光定量聚合酶链式反应(RT-qPCR)检测促血管生成因子(VEGF和bFGF)mRNA的表达。蛋白免疫印迹法(westernblot)检测核因子κB(NF-κB)和JAK/STAT信号通路的相关分子表达。结论:可持续稳定的leptin表达系统(HPMSCs/leptin)具有更好的细胞增殖、迁移和成血管潜能。HPMSCs/leptin单独培养和HPMSCs/leptin与受辐照HUVECs共培养体系中,促炎细胞因子的分泌增加与NF-κB和JAK/STAT信号通路的相互作用有关。HPMSCs/leptin可能促进X射线照射后HUVECs的成血管潜能和外周炎症反应。