青少年心理创伤特点及干预研究述评

青少年在经历巨大创伤性事件后,内心遭受重创,心理反应表现出独有特点．主要反映在：（1）情感上,抑郁程度重,焦虑情绪高．（2）认知上,对自身及世界的消极认知固着,记忆出现不同程度的障碍,特别是言语记忆受损严重．（3）意志上,表现为更封闭、退缩,生活动力相对缺失,内倾性增大．（4）行为上,易形成各种不良的习惯,同伴间的攻击行为增多．对此可以采用认知行为疗法、精神催眠辅助治疗、症状监控、多维家庭治疗技术等进行干预．     

竞赛特质焦虑干扰优势反应抑制的神经准备过程:ERP的证据

竞赛焦虑对操作表现的影响是运动心理学的重要研究领域,注意控制理论对二者关系从执行功能的角度进行了新的阐释,认为焦虑干扰执 行功能中的抑制功能。近年来,运动心理学对抑制功能探讨较多,对优势反应和任务无关刺激的抑制是抑制功能的2个重要成分,但以往研究大多 集中于抑制无关刺激,对优势反应抑制的探讨相对欠缺,而后者却是竞技场上运动员时常需要面对的问题。已有行为学研究发现,竞赛特质焦虑对 优势反应抑制功能有干扰效应,但其神经机理尚待探讨。本研究采用ERP技术探讨竞赛特质焦虑干扰优势反应抑制的神经基础,假设竞赛特质焦 虑干扰前脑区域（额区和中央区）优势反应抑制的神经准备过程。招募26名研究参与者,根据《赛前情绪量表32×6》测试得分,二分法划分竞赛特质 焦虑高分组和低分组。2组均完成代表优势反应抑制功能的反向眼跳任务,分析抑制“线索”出现前-100~0 ms时程的ERP成分,发现反向眼跳的准 备期在额区和中央区诱发出一负成分,竞赛特质焦虑高分组的波幅小于低分组。以上结果部分支持了假设,竞赛特质焦虑对优势反应抑制的神经 准备期有干扰效应,且主要由前脑区域控制,此结果为注意控制理论在竞赛焦虑领域的适用性提供了神经生理学的部分证据。     

The effect of issue obtrusiveness,issue congruence,and response strategies on the acceptance of crisis communication messages

Based on issue ownership theory, this study identifies the effects of issue obtrusiveness, issue congruence, and crisis response strategies on the level of acceptance of crisis communication messages employing 2 × 2 × 3 (issue obtrusiveness × issue congruence × response strategies: scapegoat, justification, reminder) experimental research. Two large Korean business groups (Hyundai and SK) were selected. There was a main effect in crisis response strategies and a three-way interaction effect. A crisis communication message was effective when a corporation owns congruent and unobtrusive issues. Implications for public relations research and practices were discussed.     

响应面法优化甘薯茎叶中黄酮类化合物的提取工艺

为优化甘薯茎叶中黄酮类化合物的超声提取工艺,在单因素实验的基础上,选择乙醇浓度、溶剂倍量、超声提取时间为自变量,黄酮得率为响应值,采用Box-Behnken组合设计,运用Design-Export 8.05b软件分析各自变量及其交互作用对黄酮得率的影响,建立了黄酮得率的二次多项式回归方程的预测模型。确定甘薯茎叶中黄酮的最佳提取工艺条件为:乙醇浓度60.00%、溶剂倍量35.00、超声时间40.00min,在此条件下,黄酮得率为10.3002%。本研究原料价廉易得,提取方法高效可行,分析方法可信,可为甘薯茎叶的开发利用提供依据。     

普通学校中学生特殊教育需要响应:以英国为例

采用渐进型响应模式来满足普通学校中学生的不同特殊教育需要,是英国在普通学校实践全纳教育过程中所采用的一种方法,这种模式要求各学校和教师根据学生特殊教育需要程度的不同分五阶段进行响应,为学生提供不同水平的支持,以便及时帮助学生解决学习困难,提高其教育质量。     

负反馈放大电路稳定性动态仿真研究

利用运放构建有3个极点的开环放大电路,由Multisim的AC Analysis获得该电路的频率响应,找到对应相位差为-180°的开环电压放大倍数。根据放大电路稳定工作条件,分析出反馈系数范围,据此计算出电压串联负反馈的反馈电阻,构成反馈放大电路。改变反馈电阻,用Multisim中的示波器同时观察输入输出波形,并用Fourier Analysis对输出信号进行傅里叶分析。结果显示振荡波形主要由两部分频率成分迭加而成,一部分是对输入信号放大产生的输出信号;另一部分是由于自激振荡产生的输出信号,其频率值与理论分析一致,除此之外,还包含幅值较小的其他频率成分的谐波分量。通过仿真,同时还可以观察到自激振荡的起振过程,随反馈电阻增大,振荡幅度减小到振荡消失等过程。     

随机经济周期模型的动力学研究

在文献[1]给出的动态经济周期模型的基础上,研究当O＊（t）（白发投资与自发消费的总和）为随机外力干扰的随机函数时对经济的影响,应用多尺度法及文献[7]求出了一阶近似解及系统的稳态响应,并分析了参数和随机激励的幅值对经济系统的影响．讨论了非零解的稳定性以及非零解稳定的充要条件,得出当随机激励的带宽γ较小时,系统仍会出现多值现象．     

Establishment of a production process for a novel vaccine candidate against Lawsonia intracellularis

BackgroundLawsonia intracellularis remains a problem for the swine industry worldwide. Previously, we designed and obtained a vaccine candidate against this pathogen based on the chimeric proteins: OMP1c, OMP2c, and INVASc. These proteins formed inclusion bodies when expressed in E. coli, which induced humoral and cellular immune responses in vaccinated pigs. Also, protection was demonstrated after the challenge. In this study, we established a production process to increase the yields of the three antigens as a vaccine candidate.ResultsBatch and fed-batch fermentations were evaluated in different culture conditions using a 2 L bioreactor. A fed-batch culture with a modified Terrific broth medium containing glucose instead of glycerol, and induced with 0.75 mM IPTG at 8 h of culture (11 g/L of biomass) raised the volumetric yield to 627.1 mg/L. Under these culture conditions, plasmid-bearing cells increased by 10% at the induction time. High efficiency in cell disruption was obtained at passage six using a high-pressure homogenizer and a bead mill. The total antigen recovery was 64% (400 mg/L), with a purity degree of 70%. The antigens retained their immunogenicity in pigs, inducing high antibody titers.ConclusionsConsidering that the antigen production process allowed an increment of more than 70-fold, this methodology constitutes a crucial step in the production of this vaccine candidate against L. intracellularis.How to cite: Salazar S, Gutiérrez N, Sánchez O, et al. Establishment of a production process for a novel vaccine candidate against Lawsonia intracellularis. Electron J Biotechnol 2021.https://doi.org/10.1016/j.ejbt.2021.01.002     

基于供应链响应时间的协同计划模型

在当前全球化、客户化导向的快速多变的市场环境中，供应链敏捷、快速的响应能力成为市场竞争力的重要体现。本文把供应链响应时间视为一种重要的可分配资源，力求在供应链节点企业间及节点企业内生产时间和物流时间的合理分配上提供一个有效的协同计划工具。在建立供应链整体与局部节点企业计划协同机制的基础上，对供应链响应时间进行了优化建模研究，并提出相应算法，最后用实际调研的数据作为一个算例对模型进行了数值演算和验证。     

Effect of pile-cap connection on behavior of torsionally loaded pile groups

To evaluate the responses of fixed and pinned pile groups under torsion, a method is presented to analyze the nonlinear behavior of free-standing pile groups with rigid pile caps. The method is capable of simulating the nonlinear soil response in the near field usingp-y and r-θ curves, the far-field interactions through Mindlin＇s and Randolph＇s elastic solutions, and the coupling effect of lateral resistance on torsional resistance of the individual piles using an empirical factor. Based on comparisons of the solutions for fixed- and pinned-head, 1×2, 2×2, and 3×3 pile groups subjected to torsion, it was found that pile-cap connection significantly influences the torsional capacity of pile groups and the assignment of applied torques in the pile groups. In this study, the applied torques for the pinned-head pile groups are only 44%-64% of those for the corresponding fixed-head pile groups at a twist angle of 2^o. Such a difference is mainly due to the change of the lateral resistances of individual piles in the groups.