3-(6-溴吡啶-2)-2-(2-氟-6-氯苯)-噻唑酮-4的电子结构与抗HIV活性间关系的量子化学研究

采用AM1方法,对3-(6-溴吡啶-2)-2-(2-氟-6-氯苯)-噻唑酮-4(BPFCTZO)进行了量子化学计算,给出了分子的几何构型、原子的净电荷、键级、前沿分子轨道组成与能级、前沿轨道电子密度等参数.结果表明BPFCTZO分子的稳定构型呈蝴蝶状,噻唑酮环为主活性区,其上的2位C、4位N和6位O等原子很可能是其与RT酶作用的活性位点.     

新媒体融合背景下地方性学术期刊如何走出困境——以“域出版”为中心

随着手机与互联网的紧密结合,移动出版概念应运而生,“域出版”逐渐成为学术期刊界关注的新型传播模式,期刊界对于重构学术传播新秩序的呼声也越来越高,地方性期刊对“域出版”的运用不失为改革的有效措施。“域出版”为地方性学术期刊的发展提供了有效途径,对“域出版”的应用还远没有发挥其真正的功能,学术期刊界应联合起来,共同打造“学术共同体”,积极利用“域出版”的创作功能,发挥特色栏目的优势。     

L-fuzzy相对T-1,相对T0与相对次T0分离性

定义了L-fuzzy拓扑空间中的相对T-1，相对T0与相对次T0分离性，讨论了相对T-1，相对T0与相对次T0分离性的一系列性质．证明了相对T-1，相对T0与相对次T0分离性是遗传的、传递的、弱同胚不变的及可乘性等性质，并给出了一些例子。     

“人以群分”适用于大学生的学业成就吗——基于社会网络分析和Meta分析的方法

嵌入班级网络中的大学生在交往中形成不同的小群体,这些小群体间的学业成就是否具有显著性差异?研究者将社会网络分析方法中的整体网分析与元分析(Meta分析)相结合,在对大学生班级内小群体的学业成就差异进行多项独立研究的基础上,运用元分析技术将29项研究(N=1076)的结果予以综合分析,得到如下结论:(1)每个班级的大学生都在互动过程中形成了不同数量的小群体,且每个小群体中的学生数量不同;(2)大学生学业成就存在着“人以群分”现象,即同一班级的大学生在互动中产生的小群体在学业成就上存在显著性差异。因此,大学生应加强与高学业成就水平的小群体之间的互动以提高学业成就;而高校管理者也应采用科学的方法识别班级中的不同小群体,从而采取相应的学业成就提升策略。     

合成草莓酯的新型催化剂

综述了无机盐、无机酸、杂多酸(盐)、固体超强酸等催化剂催化合成草莓酯的实验情况.结果表明,硫酸铝、CuCl2 ·2H2 O、固体超强酸SO2 -4 -TiO2 /Al2 O3、活性炭固载硅钨酸等催化剂催化合成草莓酯的收率较高,具有一定的工业应用价值.     

R&D co-operation and innovation a microeconometric study on French firms

The scope of this paper is to report new empirical evidence on the determinants of R&D co-operation. Indeed, the literature on the capabilities of firms emphasises the role of knowledge in the performance and evolution of firms who use knowledge developed in others to build their own knowledge capital. R&D co-operation between firms is one of the many strategies by which this knowledge may be transmitted. Several theoretical models have stressed that R&D co-operation is more likely when the level of spillovers is high. While this supposition is used in many theoretical models, it has rarely been tested before. Our results do not lend strong support to this intuition. Indeed, our spillover variables (national pure spillovers, national rent spillovers and imports of machine tools), which were supposed to match the theoretical notion of spillovers used in these theoretical models have a positive but not a significant role when explaining R&D co-operation. The R&D co-operation increases with size and with R&D intensity, but not with market share. It also increases with the budget spent on paying license fees and on acquiring patents and labour from foreign firms. In this study, we also analyse the determinants of innovation. In more precise terms, we compare the effects of R&D co-operation to those played by traditional internal factors and those exerted by external, pure and rent spillovers on the innovation capacity of the firms. Our results show that the commercial success of innovations of French firms depends mainly on size, market share, R&D intensity and human capital. Inward FDI from industrialised countries exerts a positive and significant effect. The finding highlights the important role of the absorptive capacity of firms. Spillover measurements, such as the acquisition of machine tools, foreign patents, licenses, and technological opportunities have a positive impact on innovation.     

Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro

BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006     

“大学域” 图书馆资源共享研究

随着高等教育规模的扩大，大学城在我国得到了迅速的发展。文章分析了大学城在我国产生的必然性，大学城资源共享的主要领域，提出了大学城图书馆之间可以在政策制定、协调采购、目录编制、馆际互借、对外交流和利用现代信息技术方面实现资源共享，分析了在资源共享方面存在的问题。     

新布客书屋:托起贫困地区儿童的希望

"新布客书屋"充实了贵州省贫困地区儿童的阅读资源,为贵州扶贫工作锦上添花。文章论述新布客建立的缘起、新布客品牌以连锁概念运营的历程,探究新布客的构建、内涵、统一运营模式、志愿服务,以及公共馆文化服务在精准扶贫中的作用。     

基于5W1H分析的公共图书馆大字本图书阅读推广服务探究

大字本图书是低视力人群重要的信息来源和学习资源,公共图书馆作为大字本图书主要的馆藏地,肩负着大字本图书阅读推广服务的重要职责。通过5W1H分析法,提出公共图书馆大字本图书阅读推广服务对策,包括服务时间、地点的选择、服务团队的建设以及服务方法等,以期对公共图书馆开展大字本图书阅读推广服务有所帮助。