56例进展期胃癌患者SIMA、CEA、CA50、CA19－9联合检测评价

目的对56例进展期胃癌患者进行4种血清肿瘤标记物联合检测评价,探索优选检测项目,提高诊断的灵敏度、准确性.方法应用酶免双抗夹心法测定SIMA和应用放射免疫方法测定CEA、CA50、CA19-9,共测定正常对照组33例健康成人和进展期胃癌56例.结果胃癌组4种血清肿瘤标记物均与正常对照组差异有显著性意义(p＜0.05),其中正常对照组SIMA为(8.09±1.94)×103u/L,胃癌组SIMA为(90.47±28.51)×103u/L.SIMA的灵敏度和准确性高于其它3种,与CEA灵敏度相比,差异无显著意义(p＞0.05),SIMA与CEA组合灵敏度升高至81.76%,高于单项灵敏度,高于其它组合灵敏度,差异有显著意义(p＜0.05).结论SIMA是一种敏感的新型血清肿瘤标记物,SIMA和CEA可以作为联合检测胃癌的首选标志.     

旋毛虫肌肉幼虫可溶性抗原成份的分析

本文用不连续聚丙烯酰胺凝胶电泳(Disc—PAGE)和十二烷基硫酸钠—聚丙烯酰胺凝胶电泳(SDS—PAGE)对旋毛虫肌肉幼虫的可溶性抗原进行了分析。结果表明经Disc—PAGE电泳后可辨认出16条带。经SDS—PAGE电泳后可辨出22条带,其中有6条主带,文中对各主带在保护性免疫及免疫诊断中的意义进行了讨论。     

高致病性禽流感:不见硝烟的阻击战

遥远的东北,伴随2005年初冬的第一场雪,高致病性禽流感的来势似乎比寒流更猛一些,以H5N1型病毒为主的禽间疫情高度活跃,抗原变异重组,对人群构成严重威胁,全球迄今已确诊150多例H5N1病例,死亡高达51%。病毒随候鸟迁徙,穿城越国地远程飞散似乎刚刚拉开序幕,一场严峻的病毒阻击战,虽不见硝烟,却异常艰难。     

中医对动脉粥样硬化红细胞免疫的调节

动脉粥样硬化(atherosclerosis,AS)是一种多因素作用协同刺激机体所产生的病理过程。动脉粥样硬化的形成与红细胞免疫关系十分密切。红细胞上有许多与免疫有关的物质,如CR1、DR3、LFA—3(淋巴细胞功能相关抗原—3)、DAF(补体衰变加速因子)、SOD(超氧化物歧化酶)、CD58、CD59、HRF(组织释放因子)等,因而具有识别、粘附、浓缩抗原、清除循环免疫复合物(CIC)等能力,参与机体的免疫调控。循环免疫复合物(CIC)可通过经典途径或旁路激活粘附C3b,CIC主要由红细胞清除,即红细胞通过其表面的C3b受体温表与免疫复合物中(IC)补体成分结合…     

自身免疫耐受性

免疫系统的基本功能是识别自身和非己,也就是说,在正常情况下,免疫系统可以精确地认识和区分自身的还是外来的抗原成分。对外来的抗原则发生免疫反应,即把它们局限起来、抑制住、排除掉或消灭之。对自身抗原则不会产生这类免疫应答。机体何以能够识别自身和非己呢?为什么在正常情况下机体免疫系统不攻击自身组织抗原呢?本文就谈谈自身免疫耐受性问题。什么是自身免疫耐受性呢?我们知道,抗原刺激机体,通常可以引起免疫应答,表现为特异性抗体或致敏淋巴细胞的形成。但是,在某些条件下,机体接受抗原(自身的或外来     

On dendritic cell-based therapy for cancers

Dendritic cells (DCs), the most prevalent antigen-presenting cell in vivo, had been widely characterized in the last three decades. DCs are present in almost all tissues of the body and play cardinal roles in recognition of microbial agents,autoantigens, allergens and alloantigen. DCs process the microbial agents or their antigens and migrate to lymphoid tissues to present the antigenic peptide to lymphocytes. This leads to activation of antigen-specific lymphocytes. Initially, it was assumed that DCs are principally involved in the induction and maintenance of adaptive immune responses, but now it is evident that DCs also have important roles in innate immunity. These features make DCs very good candidates for therapy against various pathological conditions including malignancies. Initially, DC-based therapy was used in animal models of cancers. Data from these studies inspired considerable optimism and DC-based therapies was started in human cancers 8 years ago. In general,DC-based therapy has been found to be safe in patients with cancers, although few controlled trials have been conducted in this regard. Because the fundamentals principles of human cancers and animal models of cancers are different, the therapeutic efficacy of the ongoing regime of DC-based therapy in cancer patients is not satisfactory. In this review, we covered the various aspects that should be considered for developing better regime of DC-based therapy for human cancers.     

科学家研发出新的“基因捕获技术”

11月28日,华大基因和罗氏公司联合宣布已成功研发出全新人类白细胞抗原系统区域基因捕获技术,首次实现了对人类白细胞抗原系统区域的高度覆盖。此技术和新一代高通量技术的完美结合,将有助于科研人     

离奇的血型之谜

一种从未见过的血型突然被发现,人们吃惊之余又伴随着一些恐惧——这血型从哪里来?正常的血型都是从父母那里遗传得来,新血型是突然变异的结果吗?是新人种要出现?还是隐藏着无法预计的来自外星的灾难?     

基因工程疫苗

基因工程疫苗是用基因工程方法或分子克隆技术,分离出病原的保护性抗原基因,将其转入原核或真核系统使表达出该病原的保护性抗原,制成疫苗,或者将病原的毒力相关基因删