A Single Centre Prospective Study of Liver Function Tests in Post Liver Transplant Patients

Liver transplantation means surgical replacement of a diseased liver with a healthy liver. The survival rate used to be 30 % after 1 year and LTx was considered to be the last procedure when all medical or surgical intervention failed. Advances in donor organ preservation, surgical techniques, patient selection, immunosuppressive regimens and treatments for opportunistic infections all have contributed to substantially improve the survival rates. Despite substantial technological, medical and surgical advances, liver transplantation remains a complex procedure that is accompanied by significant morbidity and mortality. The post-operative outcome of each patient varies greatly depending on the patient’s pre- operative state, quality of the donated organ and the complexity of the surgery. Complications occur both immediately post transplant and in the long term. Most of the problems can be satisfactorily assessed with a panel of routine LFTs results of which are generated quickly, cheaply on the analyzer which operates 24 h. Liver Function Test identifies the presence of problem but not problem itself. Abnormal results can be meaningful only when used with clinical data, radiological findings. The study includes 75 post LTx patients in three groups adults (non ACR), Pediatrics and ACR. All recipients were on immunosuppressive therapy (tacrolimus, mycophenolate and methylprednisolone), antiviral (ganciclovir), antiprotozoal, antibacterial and antifungal (fluconazole). 5 mL of blood was drawn in plain vacutainer from the post LTx patients every day for 15 days and LFT and GGT was done. Routinely performed liver function tests correlates well with clinical complications involving liver in the transplant patients. Instead of daily testing, may be alternate day analysis of LFT should be sufficient for effective monitoring of patients. The total protein and albumin and the transaminases offer little help in monitoring LFT post LTx. The elevated levels of serum GGT and ALP may be related to chronic immune damage to the transplanted liver. Serum GGT and ALP can be used as early markers for diagnosing biliary complications and can be used to asses adequacy of endoscopic treatment in the group of patients presenting early. Thus, most of the problems can be satisfactorily assed with a panel of routine LFTs generated quickly, cheaply on analyzer which operates 24 h each day. However, it must be emphasized that LFTs may identify the presence of problems but not the problem itself and the abnormal results are meaningful only when correlated with other clinical information.     

中年亚健康人群血清生化指标研究

目的：了解中年亚健康人群的血清生化指标特点。方法：以筛选出的中年（35～55岁）亚健康人群为研究对象（203例,其中男性131例,女性72例）,以中年健康人群作为对照（40例,其中男性20例,女性20例）,检测其空腹血尿酸（UA）、血糖（GLU）、血脂及肝功能相关的丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）等生化指标,并分析各生化指标的特点。结果：中年亚健康人群的UA、TC、TG、TC/HDL-C和LDL-C水平均显著高于健康对照组,且亚健康男性高于同年龄段的亚健康女性（P〈0.05）,HDL-C水平显著低于健康对照组,且亚健康男性低于同年龄段的亚健康女性（P〈0.05）;GLU水平均无显著性差异（P〉0.05）;亚健康男性的TG水平达1.86±0.94 mmol/L,明显高出正常血脂上限水平1.7 mmol/L,表现出边缘性升高;亚健康组与健康组的ALT、AST、ALT/AST等肝功能指标无显著性差异,但是亚健康男性的ALT、AST、ALT/AST和ALP显著高于亚健康女性;亚健康男性三个年龄段的UA水平均高于正常范围;随着年龄的增长,亚健康人群的TG及TC/HDL-C水平均有增高的趋势,HDL-C则呈下降的走势。结论：中年亚健康男性的血尿酸和血脂等生化指标表现出异常的趋势。     

衰竭运动小鼠肝损伤的实验性研究

本文通过小鼠衰竭运动后，取其肝脏，用组织学、生物化学方法，研究了小鼠衰竭运动后在形态和功能方面的改变。结果提示７次衰竭运动的自由基代谢失衡，ＡＬＴ升高以及肝细胞形态学的改变对肝损伤的发生发展起重要作用。     

支链氨基酸对不同负荷运动后丙氨酸-葡萄糖循环影响的研究

采用实验法，探讨支链氨基酸(BCAA)对划船运动员不同负荷运动后丙氨酸——葡萄糖循环的影响。结果表明，补充BCAA影响丙氨酸-葡萄糖循环代谢中酶的活性，促进运动中及恢复期丙氨酸-葡萄糖循环，有利于糖的异生、提高运动耐力、延缓疲劳发生和促进运动后疲劳的消除。