A rapid method for plasma ammonia estimation using an indigenously purified enzyme

Determination of ammonia level in blood is important, especially in the diagnosis of hepatic disorders. An indigenously purified enzyme was used in the standardisation of the assay. The assay is a two reagent system, requires five minutes for completion and can be performed at temperature between 25–27°C. Performance of the assay was assessed by linearity, imprecision, functional sensitivity and interference studies. Lyophilised reagent I and reagent II were found stable for at least one year. The plasma level of ammonia for the controls was 13.7±7.3 μMol/L, whereas for subjects of hepatic disorders, it was 69.1±32.4 μMol/L (P<0.001). The functional sensitivity was between 2–1000 μMol/L. Within-run coefficient of variation was between 1.1–2.0% and between-run coefficient of variation was between 1.9–3.7%. The mean recovery after dilution was 99.6%. The present method can estimate ammonia up to 1000 μMol/L without dilution of sample. Assay time of five minute may be shortened to one minute. This method is suited for routine clinical use in treatment of hepatic disorders.     

SRB显色法用于抗癌药物敏感性试验的研究

采用ＳＲＢ显色法对９６孔培养板中短期培养的人大肠癌细胞株Ｃｏｌｏ２０５、人骨肉瘤细胞株ＯＳ７３２,人舌癌细胞株Ｔｃａ８１１３经５－ＦＵ,顺铂,蟾酥等药物处理后的活性进行了研究。结果表明,ＳＲＢ法对这些细胞株的体外抗癌药物敏感试验结果稳定,该法与其它细胞毒试验相比有一定优点,值得推广应用。     

体育活动作为考试应激的应付方法研究

选择特定的考试应激情境，使用心境状态量表（POMS）和锻炼感觉量表（EFI），通过实验比较的方法，目的为研究体育活动作为应付方法降低考试应激的效果，实验结果表明，参与体育活动能够有效地降低考试后产生的应激反应，改善心境状态；体育活动时心境状态的改善与运动愉快感的产生之间存在显著意义的相关关系。     

信息查寻活动中的合作行为与合作式信息查寻

指出了传统信息查寻和情报检索研究只针对个体用户的行为,忽视查寻的社会情景以及用户之间的相互关系的问题。对信息查寻和情报检索中的合作行为进行了研究,介绍了合作系统的发展状况,并对今后的发展趋势进行了分析。     

用多元智能理论设计综合实践活动课

本文在介绍多元智能理论以及教改新增的综合实践活动课的基础上，分析了多元智能理论与综合实践活动课的关系，并通过一个案例提出用多元智能理论设计综合实践活动课的方法。     

电子效应对有机反应的影响

讨论了电子效应对有机化学反应方向、活性及历程等方面的影响。     

Validity of SenseWear® Armband v5.2 and v2.2 for estimating energy expenditure

We compared SenseWear Armband versions (v) 2.2 and 5.2 for estimating energy expenditure in healthy adults. Thirty-four adults (26 women), 30.1 ± 8.7 years old, performed two trials that included light-, moderate- and vigorous-intensity activities: (1) structured routine: seven activities performed for 8-min each, with 4-min of rest between activities; (2) semi-structured routine: 12 activities performed for 5-min each, with no rest between activities. Energy expenditure was measured by indirect calorimetry and predicted using SenseWear v2.2 and v5.2. Compared to indirect calorimetry (297.8 ± 54.2 kcal), the total energy expenditure was overestimated (P < 0.05) by both SenseWear v2.2 (355.6 ± 64.3 kcal) and v5.2 (342.6 ± 63.8 kcal) during the structured routine. During the semi-structured routine, the total energy expenditure for SenseWear v5.2 (275.2 ± 63.0 kcal) was not different than indirect calorimetry (262.8 ± 52.9 kcal), and both were lower (P < 0.05) than v2.2 (312.2 ± 74.5 kcal). The average mean absolute per cent error was lower for the SenseWear v5.2 than for v2.2 (P < 0.001). SenseWear v5.2 improved energy expenditure estimation for some activities (sweeping, loading/unloading boxes, walking), but produced larger errors for others (cycling, rowing). Although both algorithms overestimated energy expenditure as well as time spent in moderate-intensity physical activity (P < 0.05), v5.2 offered better estimates than v2.2.     

有机气体麻醉活性的QSAR研究

应用分子力学方法MMP和半经验量子化学AH1法得到了18种有机气体的优势构象,利用量子化学算法和分子图形学技术获得电子结构、几何结构和拓扑结构参数,并将这些参数与有机气体的麻醉活性参数相关联。结果表明：有机气体的麻醉活性与分子的极化率和辛醇／水分配系数的相关性较好,成功地建立了18种有机气体的构效关系武。     

Division,result and score margin alter the physical and technical performance of elite wheelchair tennis players

ABSTRACT

This study determined the physical and technical demands of elite wheelchair tennis (WT) match-play, how the demands differed between divisions (Men, Women, Quad) and the effect that set result and score margin had on these demands. Seventeen WT players were monitored during a singles competition. Physical measures of performance were analysed using an indoor tracking system and inertial measurement units. Technical measures of performance were examined using video analysis. Physical measures of performance differed by division (Men > Women > Quad) for most parameters. Rallies were longer during Men’s (P = 0.027) and Women’s (P = 0.004) matches compared to Quad’s and fewer shots were hit off 2 bounces in Men’s matches compared to Women’s and Quad’s (P ≤ 0.026). High-speed activity (HSA) increased during losing sets (P = 0.043). Most physical measures of performance increased by moderate to large effects during sets with a small score margin (≤3 games differential). Mean speed and HSA were similar during losing sets, regardless of margin, but decreased (large effects) when winning by a large margin. This study demonstrated the physical and technical demands that elite WT players need to be prepared for and how situational factors can influence these demands.     

政策工具视角下《身体活动全球行动计划(2018-2030)》文本分析及对我国全民健身政策制定的启示

采用内容分析法，按照政策工具的分析框架从X基本政策工具和Y统筹协作多元主体两个维度对《身体活动全球行动计划（2018-2030）》的政策文本进行分析单元界定、内容编码、计量与分析。研究发现：世卫组织《行动计划》政策文本兼顾了三种类型的政策工具，运用较为均衡，推动和拉动及影响效用较为显著。其中供给型政策工具内容占据主导，重点关注科技的使用与创新；环境型政策工具内容运用合理，长短期政策相互配合；需求型政策工具内容比例适中，更为侧重国际交流与服务外包。该计划以公共健康为本，注重跨区域跨部门合作，发挥社区组织为支撑的多元主体协作机制，以推动全民健身的开展与实施。最后，针对《行动计划》政策文本中政策工具运用情况的分析，以期为我国全民健身政策的优化与完善提供启示与借鉴。