Effect of microcystin-LR on protein phosphatase 2A and its function in human amniotic epithelial cells

Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However, the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation. To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity of MCLR.     

教育财务管理信息系统设计

为提高学校的信息化管理水平,本文结合学校的业务需求和实际工作情况,研究并设计了适合学校的"教育财务管理信息系统",以期提高学校自身的财务管理效率,为学校的各项行政管理活动以及教学管理提供相关保障,从而促进学校更快更好地发展。本文基于软件工程面向对象的思想,采用统一建模语言UML进行财务管理系统的分析、设计和实现。     

基于.NET的大学生创业信息服务平台建设与规划研究

本文根据大学生创业园信息管理的需求,采用.NET技术,构建了一套基于B/S三层架构的综合信息服务管理平台。利用该平台,可以实现大学生创业管理信息化,为大学生创业提供更好的项目来源和技术支撑,提升大学生创业服务能力,整合各类创业资源,有效提高大学生创业的成功率。     

Open access to data: An ideal professed but not practised

Data-sharing is an essential tool for replication, validation and extension of empirical results. Using a hand-collected data set describing the data-sharing behaviour of 488 randomly selected empirical researchers, we provide evidence that most researchers in economics and management do not share their data voluntarily. We derive testable hypotheses based on the theoretical literature on information-sharing and relate data-sharing to observable characteristics of researchers. We find empirical support for the hypotheses that voluntary data-sharing significantly increases with (a) academic tenure, (b) the quality of researchers, (c) the share of published articles subject to a mandatory data-disclosure policy of journals, and (d) personal attitudes towards “open science” principles. On the basis of our empirical evidence, we discuss a set of policy recommendations.     

第一性原理方法研究小芳香烃分子的基态性质

采用基于密度泛函(DFT)和二阶多体微扰理论(MP2)的第一性原理方法对小芳香烃分子及其自由基的几何结构进行优化。计算结果表明,DFT中的B3LYP方法得到的最优化结构与实验结果符合得最好。相比大基底cc-pVTZ,小基底6-31G(d)得到的几何结构、能量与实验值符合得好。B3LYP方法计算得到氧原子在(d)位置的五苯环(C19H10O)体系最稳定。对小体系适用的理论方法(B3LYP)可以用来研究多环芳香烃(PAH)的性质。     

The happy-fish-little-pond effect on enjoyment: Generalizability across multiple domains and countries

Academic enjoyment is an important educational construct given that it benefits students' engagement, persistence, wellbeing, and mental health. In this study, we examine two factors that determine this crucial emotion, namely student- and class-level achievement. Past research has been restricted to single-country or single-domain examinations of secondary school students, limiting generalizability of findings. To bridge this gap, we utilize the Trends in International Mathematics and Science Study and the Progress in International Reading Literacy Study (TIMSS-PIRLS) combined 2011 data (N = 180,084 4th-grade students, 37 countries). Our results provide robust evidence that student-level achievement positively predicts enjoyment in math, science, and reading, while the effects of class-level achievement are negative—the Happy-Fish-Little-Pond Effect. These results showed relative universality across the domains and countries examined.     

基于C／S和B／S的施工图审查信息系统

结合一个实际项目介绍了基于C／S和B／S的建设工程施工图审查信息系统的设计与实现,具体讨论了系统的结构设计与系统功能划分,以及在实现过程中涉及的关键技术。     

基于C/S与B/S模式的运动会信息管理系统设计和实现

分析了广泛应用于软件项目设计的C／S、B／S模式以及两种模式各自的优点与不足，并采用这两种模式相结合的方法对运动会信息管理系统进行了规划与设计。     

骨骼肌抗氧化作用的信号转导机制

现已证明骨骼肌收缩诱导产生的活性氧（ROS）可造成骨骼肌氧化应激水平升高,同时肌肉的抗氧化防御系统的应答性也升高。而NFκB和MAPK是两条主要的氧化应激敏感性信号转导通路,可激活许多酶和蛋白质的基因表达,而这些酶和蛋白质在维持细胞内氧化还原稳态中发挥重要作用。综述讨论上述两条信号转导通路及其适应的生物学差异。     

基于B/S模式的综合信息系统集成平台的安全性措施

管理信息系统发展到综合信息系统集成阶段后,安全问题成为系统集成工作中必须考虑的一个至关重要的问题。基于B/S模式的综合信息系统集成平台的安全性模型及该模型的具体实现方法、路线,在多用户的Web环境下,既能满足用户的需求又能最大限度地保证系统的安全,具有较强的实用性。