营销组合4P范式的不可替代性

营销组合范式从4P发展至4C、4R,引起学者的热烈反应和广泛讨论。在前人研究的基础上,将4P、4C、4R从产品、定价、渠道、促销四个方面进行对比,得出4P理论是具有实操性的营销组合工具,能为消费者提供实际的价值方案,更符合营销工具的实质,是营销学不可替代的理论根基。     

Sequence- and structure-selective mRNA m5C methylation by NSUN6 in animals

mRNA m⁵C, which has recently been implicated in the regulation of mRNA mobility, metabolism and translation, plays important regulatory roles in various biological events. Two types of m⁵C sites are found in mRNAs. Type I m⁵C sites, which contain a downstream G-rich triplet motif and are computationally predicted to be located at the 5^′ end of putative hairpin structures, are methylated by NSUN2. Type II m⁵C sites contain a downstream UCCA motif and are computationally predicted to be located in the loops of putative hairpin structures. However, their biogenesis remains unknown. Here we identified NSUN6, a methyltransferase that is known to methylate C72 of tRNA^Thr and tRNA^Cys, as an mRNA methyltransferase that targets Type II m⁵C sites. Combining the RNA secondary structure prediction, miCLIP, and results from a high-throughput mutagenesis analysis, we determined the RNA sequence and structural features governing the specificity of NSUN6-mediated mRNA methylation. Integrating these features into an NSUN6-RNA structural model, we identified an NSUN6 variant that largely loses tRNA methylation but retains mRNA methylation ability. Finally, we revealed a weak negative correlation between m⁵C methylation and translation efficiency. Our findings uncover that mRNA m⁵C is tightly controlled by an elaborate two-enzyme system, and the protein-RNA structure analysis strategy established may be applied to other RNA modification writers to distinguish the functions of different RNA substrates of a writer protein.     

Discussion on National Standard GB 6167"Methods for Testing the Performance of Airborne Particle Counter

Classificationofaircleanlinessinanykindofclean roomsandcleanzoneswillbeaccomplishedintermsofconcentrationofairborneparticles .Alightdiscreteair borneparticlecounter (hereafterreferringtoasAPC)isusedtodeterminetheconcentrationofairborneparticlesequaltoandlargerthanthespecifiedsizesatdesignatedsamplinglocation[1,2 ] .Inthepracticalmeasurementofparticleconcentrationincleanrooms,itisfoundthetest ingresultsofparticlenumberinthesameareacountedbydifferentparticlecountersarequitedifferentanduptoanord…     

三层C/S结构报刊发行信息系统的设计实现

阐述了三层C/S体系结构的优点,Tuxedo中间件的主要功能和特点.介绍了在Linux操作系统下基于Tuxedo的三层C/S体系结构的报刊发行信息系统的设计与实现.     

The industrial connections in Donald Judd's art

This paper examines the context and impact of the industrial materials and processes of the mid-twentieth century on Donald Judd's works, focusing on galvanized steel, cellulose nitrate paints, anodizing, and the RAL color matching system. Exploration of the industrial materials and methods Judd utilized provides a greater understanding of his processes, choices, and ultimately his art, its care, and legacy.     

智慧城市信息安全风险评估模型构建与实证研究

[目的/意义] 面对智慧城市日益突出的信息安全问题,提出一个科学的风险评估模型,以快速对智慧城市进行风险分类并分析风险因素。[方法/过程] 从智慧城市信息安全的5个层次和风险评估的4个主要因素出发,构建智慧城市信息安全风险指标框架,对智慧城市实例进行ward系统聚类形成分类属性,选择C4.5算法的决策树方法建立风险评估模型,并验证模型的科学性。[结果/结论] 结果发现:安全管理制度、数据泄露与损坏威胁、安全意识薄弱性和设备安全漏洞是智慧城市之间差距最显著的风险因素,根据评估结果提出保障智慧城市信息安全的建议。     

潮州产10种野菜中硝酸盐、亚硝酸盐及Vc的含量

利用分光光度法测定了潮州产10种野菜中硝酸盐、亚硝酸盐的含量，利用氧化还原滴定法测定了维生素ca含量．结果表明：月光花、决明、铁苋菜和叶下珠的硝酸盐含量低于轻度污染水平（432mg／kg鲜重），亚硝酸盐含量低于无公害蔬菜亚硝酸盐含量的限量标准（44．0mg／kg鲜重），它们属于一级野菜，可以安全食用；鸡矢藤、白勒和苎麻的硝酸盐含量低于785mg／kg，属于中度污染水平，亚硝酸盐含量低于无公害蔬菜亚硝酸盐含量的限量标准，属二级蔬菜范围，不宜生食，煮熟或盐渍可安全食用；鳢肠硝酸盐含量高于785mg／蚝而低于1440mg／kg，亚硝酸盐含量低于无公害蔬菜亚硝酸盐含量的限量标准，维生素C含量高于50mg／100g鲜重，属于三级蔬菜，不可生食和盐渍，可熟食。牡荆和肖梵天花虽然硝酸盐含量很低，但维生素C含量较低，亚硝酸盐含量高于无公害蔬菜亚硝酸盐含量的限量标准，所以不宜食用．     

谈义乌市公示语的英译

良好的国际化语言环境是国际化城市建设的保证。义鸟市公示语英译还存在着浮于表面的解读、选词欠地道、简单的字字对应、不规范的书写等不足。针对这些问题，建议采取建立行政管理机构、推行翻译人员持证上岗制度、加强公众舆论监督等具体措施。     

C语言的指针

以具体实例详细介绍了C语言指针复杂说明的理解方法;分析指针使用中的常见错误及解决方法;利用指针和动态分配函数实现程序运行中堆的自由空间的支配和释放。     

用单片微机8051设计的防盗报警系统

利用单片微机8051设计的防盗报警系统,包括硬件设计和软件设计两部分。该系统可用于宾馆、仓库等,它 能对受监测点进行自动监测,一旦出现盗情,能立即报警,并指示被盗的地点。