运动对RBP4诱导的胰岛素抵抗大鼠心肌Na^＋-K^＋-ATP酶及胸腺细胞凋亡的影响

研究运动对RBP4诱导的胰岛素抵抗大鼠心肌Na^＋-K^＋-ATP酶及胸腺细胞凋亡的影响。方法：8周龄雄性SD大鼠给予重组RBP4 3μg/g体重腹腔注射,12小时注射一次,持续注射4周。设RBP4＋运动组（RE）、RBP4安静组（RR）和正常安静对照组（C）,RE组予4周无负重游泳训练,60min/天。免疫组化和免疫印迹法分别检测血清RBP4,胰岛素抵抗指数（HOMA-IR）。采用化学比色法,以考马斯亮蓝G250进行蛋白定量检测心肌细胞膜Na^＋-K^＋-ATP酶、Ca^2＋-ATP酶的活性。双抗体夹心ABC-ELISA法检测血清GM-CSF含量;S-P一步法检测血液中CD4、CD8的活性。运用TUNEL法标记各组大鼠胸腺的凋亡细胞。结果：RE组血清RBP4和HOMA-IR显著低于RR组。RE组较RR组大鼠CD4细胞数增多（P〈0.05）,CD8细胞数稍有减少（P〈0.05）,CD4/CD8比值明显增加（P〈0.01）;RE组心肌细胞膜Na^＋-K^＋-ATP酶活性显著高于RR组,与对照组相比无显著差异;RE组与RR组比胸腺指数（P〈0.05）、脾脏指数明显提高（P〈0.01）。RE组在胸腺皮质及髓质部位见棕红色的凋亡细胞似较正常安静对照组增多,但较C组明显减少。结论：运动能够提高胰岛素抵抗大鼠Na^＋-K^＋-ATP酶活性,降低了细胞内Na＋浓度,明显提升RBP4诱导的胰岛素抵抗大鼠心肌传导能力;运动能够使CD4/CD8比值增加,胸腺细胞凋亡减少,能够提高RBP4诱导的胰岛素抵抗大鼠的免疫能力。     

中国图书馆学情报学期刊现代化建设研究

从编辑办公自动化软件的应用情况、期刊全文光盘出版情况、期刊上网情况3个方面对我国图书馆学情报学期刊的现代化建设现状进行分析和比较研究,在此基础上提出我国图书馆学情报学期刊建设的解决方案：转变观念,建立现代化编辑人才队伍;建立编辑管理信息系统,实行电子编务;适当出版全文光盘,丰富刊物的出版形式;利用多种渠道上网,全方位推广期刊。     

图书馆附书光盘下载系统的设计和实现

分析现有图书馆附书光盘管理系统的不足并提出解决方案,在此基础上利用软件与数据库技术设计和实现图书馆附书光盘下载系统,方便读者利用图书馆附盘图书。     

多媒体课件转VCD的制作方法

介绍了一种在电视机上实现多媒体教学的方法。     

有氧运动对人体细胞免疫功能影响的实验分析

应用实验室相关指标（细胞免疫功能）监测有氧运动对个体免疫力的影响,将36例乙型肝炎病毒核心抗体阳性的健康体检者平均分为有氧运动组和对照组,2年前后均采用化学发光法检测实验者的乙肝两对半模式、荧光定量PCR（FQ-PCR）仪测定其乙肝DNA水平和流式细胞仪检测CD4/CD8,得到有氧运动组乙肝两对半模式转为表面抗体阳性者显著高于对照组（P〈0.025）,其血清IgG、IgA含量也高于对照组,CD4/CD8的比值平均上调10.2%的结果,证明了有氧运动能明显提高个体的免疫力.     

Correlation of matrix metalloproteinase-2, -9, tissue inhibitor-1 of matrix metalloproteinase and CD44 variant 6 in head and neck cancer metastasis

INTRODUCTIONHeadandneckcancersquamouscellcarci noma (HNSCC)isthefifthmostfrequentmalig nantneoplasmworld wide ;andischaracterizedbylocalinvasion ,disseminationtolocallymphnodeandfrequentrecurrenceattheprimarysiteoftumour.Despiteimprovementsindiagnosisandt…     

浅谈随书光盘的管理与利用

本文阐述了随书光盘的特点,介绍了当前随书光盘典藏管理的几种常用方法,分析了利弊,并结合本馆实际介绍了本馆对随书光盘的管理和利用。     

CD44v6、nm23-H1在大肠癌中的表达与预后的关系

[目的]探讨CD44v6及nm23-H1蛋白的表达与大肠癌的临床病理意义以及其对大肠癌预后判断的价值.[方法]应用免疫组化技术对52例大肠癌进行上述指标的表达及对比研究.[结果]大肠癌中CD44v6及nm23-H1的阳性表达率分别为65.4％及36.5％.CD44v6与分化程度有关,而nm23-Hl与分化程度无关.二者与肿瘤临床Duke's分期及淋巴结转移密切相关(P<0.05).[结论]CD44v6及nm23-H1的联合检测对判断大肠癌预后有参考价值.     

Role of CD97stalk and CD55 as molecular markers for prognosis and therapy of gastric carcinoma patients

Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corresponding normal mucosal tissue, collected from 39 gastric carcinoma patients, were examined by immunohistochemistry and RT-PCR for the expression of CD97 and CD55. Results: CD97^stalk was strongly stained on scattered tumor cells or small tumor cell clusters at the invasion front of gastric carcinomas. The expression of CD97^stalk was frequently observed in tumors of stage I and T1 gastric carcinoma patients. The expression of CD97^stalk between Stage I and Stage II, III, IV specimens showed significant difference (P<0.05), between T1 and T2, T3, T4 specimens also showed significant difference (P<0.05). Specimens with tumor invasion depth limited in mucosa of T1 specimens showed higher positive CD55 expression than specimens with the same tumor invasion depth in T2, T3, T4 specimens, the expression of CD55 between T1 and T2 T3, T4 specimens was significantly different (P<0.05). There was strong correlation between the distribution patterns of CD97^stalk and CD55 on tumor tissues (r=0.73,P<0.05). Signet ring cell carcinomas frequently contained strong CD97^stalk and CD55-staining. Conclusions: Our results suggest that CD97^stalk is probably involved in the growth, invasion and aggressiveness of gastric carcinomas by binding its cellular ligand CD55. CD97^stalk and CD55 could be useful as molecular markers for prognosis and therapy of gastric carcinoma patients. Project (No. 2004C34010) supported by the Science and Technology Bureau of Zhejiang Province, China