半相合供者淋巴细胞输注抗中、高危非霍奇金氏淋巴瘤的实验研究

目的阐明半相合供者淋巴细胞输注（DLI）抗中、高危非霍奇金氏淋巴瘤（NHL）的机制。方法通过预实验确定输注最佳时间及最佳浓度,并检测此条件下加入去CD8^＋T细胞和去CD4^＋T细胞半相合DLs培养后细胞的凋亡率及培养液中白细胞介素-2（IL-2）的浓度。培果在最佳输注时间（培养后24小时）、最佳浓度（2．5×10^5／ml）下加入去CD8^＋T细胞半相合DLs进行培养,淋巴瘤细胞凋亡率（17．6±2．5）％与对照组（4．6±1．2）％及加入去CD4^＋T细胞半相合DLs（5．2±1．6）％相比较,有显著性差异,而且加入去CD4^＋T细胞半相合DLs的培养液中IL-2浓度显著高于加入去CD4^＋T细胞者和对照组。结论CD4^＋T细胞在移植物抗淋巴瘤效应中可能起着比CD8^＋T细胞更重要的作用,在临床使用中,选择性去除CD4^＋T细胞,可减少或减轻移植物抗宿主病（CVHD）,并且不影响移植物抗淋巴瘤效应。IL-2在半相合DIs发挥移植物抗淋巴瘤效应过程中可能起着重要的作用。     

NE对THP-1细胞IL-6 HMGB1和CD137表达的影响

目的:探讨去甲肾上腺素对人THP-1细胞CD137、IL-6及HMGB1表达的影响.方法:加入101μmol/L的去甲肾上腺素处理THP-1细胞1小时.运用逆转录多聚酶链反应检测其CD137、IL-6及HMGB1mRNA的表达.结果:10μmol/L的去甲肾上腺素能上调THP-1细胞CD137及IL-6mRNA的表达,与对照组比较差异有显著性(P<0.01);但对HMGB1mRNA的表达没有影响.结论:去甲肾上腺素上调THP-1细胞CD137及IL-6mRNA水平.     

图书馆光盘检索系统设计研究

图书馆光盘检索系统设计方案包括系统结构设计、系统流程设计和代码设计等,为图书馆光盘检索系统提供开发的路线.并利用该设计方案,成功开发出高校图书馆光盘检索系统.     

CD44v6mRNA和HSP70mRNA在胃癌中的表达及研究

目的:检测CD44v6mRNA和HSP70mRNA在胃癌组织中的表达,探讨CD44v6mRNA和HSP70mRNA在胃癌发生发展中的作用.方法:利用核酸原位杂交技术对39例胃癌组织进行检测.结果:CD44v6mRNA和HSP70mRNA在胃癌组织表达的阳性率都为61.54%(24/39),CD44v6mRNA和HSP70mRNA在高分化管状腺癌与低分化管状腺癌中的表达都有显著性差异(P＜0.05),但HSP70mRNA在胃癌组织的表达与性别、年龄、浸润深度及淋巴结有无转移都无关(P＞0.05),而CD44v6mRNA与淋巴结有无转移有关(P＜0.05).结论:CD44v6mRNA和HSP70mRNA在胃癌组织都有较高的表达,两者均可作为评估胃癌预后的指标.     

论高校图书馆随书光盘的特点及应用

本文通过结合随书光盘的种类与特性,分析了随书光盘管理状况。根据随书光盘的特点以及书与盘的关系,对随书光盘的利用提出了自己的见解。     

CAN2.OB协议分析与改进

CAN总线是现场总线的一种，较复杂的现场总线应该既能传送实时数据，又能传送非实时数据。针对实时数据和非实时数据的传送，分别提出了分布式优先权队列动态分配晋升机制和入侵型CSMA／CD协议，解决了两种数据在CAN总线上传输的兼容问题。     

浅谈光盘技术与档案编纂手段的现代化

分析了光盘技术应用现状，着重阐述了光盘技术与档案编纂手段的有机结合的必要性及其具体结合技术环节指出光盘技术与档案编纂手段的有机结合是档案编纂的发展趋势。     

Role of CD97stalk and CD55 as molecular markers for prognosis and therapy of gastric carcinoma patients

Objectives: To explore the mechanism of development and aggressiveness in gastric carcinomas by investigating the expression and role of CD97 and its cellular ligand CD55 in gastric carcinomas. Methods: Tumor and corresponding normal mucosal tissue, collected from 39 gastric carcinoma patients, were examined by immunohistochemistry and RT-PCR for the expression of CD97 and CD55. Results: CD97stalk was strongly stained on scattered tumor cells or small tumor cell clusters at the invasion front of gastric carcinomas. The expression of CD97stalk was frequently observed in tumors of stage Ⅰ and T1 gastric carcinoma patients. The expression of CD97stalk between Stage Ⅰ and Stage Ⅱ, Ⅲ, Ⅳ specimens showed significant difference (P＜0.05), between T1 and T2, T3, T4 specimens also showed significant difference (P＜0.05). Specimens with tumor invasion depth limited in mucosa of T 1 specimens showed higher positive CD55 expression than specimens with the same tumor invasion depth in T2, T3, T4 specimens, the expression of CD55 between T1 and T2, T3, T4 specimens was significantly different (P＜0.05).There was strong correlation between the distribution patterns of CD97stalk and CD55 on tumor tissues (r=0.73, P＜0.05). Signet ring cell carcinomas frequently contained strong CD97stalk and CD55-staining. Conclusions: Our results suggest that CD97stalk is probably involved in the growth, invasion and aggressiveness of gastric carcinomas by binding its cellular ligand CD55. CD97stalk and CD55 could be useful as molecular markers for prognosis and therapy of gastric carcinoma patients.     

《中国学术期刊(光盘版)》的评价功能分析

《中国学术期刊（光盘版）》收录核心期刊的总比率只有62％,评价功能尚待完善。     

Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4＋ CD25＋ Treg cells

Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4~+ CD25~+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4~+ CD25~+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4~+ CD25~+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4~+ CD25~+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4~+ CD25~+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4~+ CD25~+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.