间歇运动激活心梗大鼠心肌NRG1-PI3K/Akt通路抑制心肌细胞凋亡

摘要：目的: 探讨心脏NRG1在间歇运动激活心梗(myocardial infarction,MI)大鼠心肌NRG1-PI3K/Akt通路抑制心肌细胞凋亡的作用。方法：雄性SD大鼠48只,随机分为假手术组(S),心梗安静组(MI),心梗+间歇运动组(ME),心梗+间歇运动+抑制剂组(MEA),每组12只。采用左冠脉前降支结扎法建立MI模型。S组手术仅穿线不结扎。ME组和MEA组在MI术后1wk进行跑台间歇运动。运动开始速度为10m/min运动10min后,速度逐渐增至25m/min×7 min,再以15 m/min×3min运动,之后依次交替进行。60min×1次/d,5d/1wk×8wk。训练结束后次日,腹腔麻醉,颈动脉插管测定LVSP、LVEDP及±dp/dtmax。之后开胸摘取心脏,进行组织学制片,Masson染色。荧光实时定量PCR测定心肌NRG1及其受体ErbB2/4表达。Western Blot法检测心肌NRG1、ErbB2/4、PI3K/Akt、Bcl-2和Bax蛋白含量。Caspase3表达及TUNEL检测观察分析心肌细胞凋亡情况。结果: MI组胶原容积百分比(CVF)和LVEDP较S组显著升高(P<0.01,P<0.01),LVSP和±dp/dtmax显著降低(P<0.01,P<0.01)。且MI后心肌ErbB4 mRNA表达显著降低(P<0.01),心肌NRG1、ErbB2和ErbB4蛋白表达显著降低(P<0.05,P<0.01,P<0.01),PI3K/Akt蛋白表达及Bcl-2/Bax比值显著降低(P<0.01,P<0.01),TUNEL阳性细胞数和Caspase3活力显著增加(P<0.01,P<0.01)。ME组CVF和LVEDP较MI组显著降低(P<0.01,P<0.01),LVSP和±dp/dtmax显著升高(P<0.01,P<0.01),心肌NRG1、ErbB2和ErbB4 mRNA及蛋白表达均显著增加(P<0.01,P<0.01,P<0.01),PI3K/Akt蛋白表达和Bcl-2/Bax比值显著增加(P<0.01,P<0.01),TUNEL阳性细胞数和Caspase3活力显著降低(P<0.01,P<0.01),且运动效应被抑制剂AG1478显著减弱。结论:间歇运动可通过激活心肌NRG1及其受体表达,上调PI3K/Akt蛋白表达,抑制心肌细胞凋亡和胶原过度增生,改善心功能。     

Bursting paradigms: a colour wheel of practice-research

ABSTRACT

In this article, I question practice as a research paradigm by exploring its position in relation to non-positivist qualitative methodologies. Frayling’s [1994. Research in art and design. Royal College of Art Research Papers, 1(1), 1993/4] distinctions between research into, through, and as (for) practice are expanded to explore overlaps between these approaches. I argue for the need to understand the nuances of different epistemologies and ontologies that underpin diverse disciplinary approaches to practice-research. This is done through an analysis of a selection of Arts and Humanities Research Council (AHRC)-funded projects to find out how they are using and/or embedding practice in the research process. The resulting Colour Wheel of Practice-Research illustrates a spectrum of positions of practice in relation to research, suggesting existing research paradigms are bursting at the seams and that the “disciplinary matrix” of practice might offers other ways of knowing. The reason I have chosen to focus primarily on AHRC-funded projects is because it explicitly states its support of “practice-led research” and that it “remains dedicated to this area of research”. This article aims to add to knowledge of how and why practice-research is of continuing interest to research councils, universities, and those identifying as practice-based/led researchers.     

Kinship,Camaraderie and Contestation: Fāṭimid Relations with the Ashrāf in the Fourth/Tenth Century.

The founding of the Fā?imid caliphate across the southern Mediterranean, and then in Egypt, Syria and the ?ijāz at the turn of the fourth/tenth century, necessitated its negotiation with the ashrāf, those who claimed lineal descent from the Prophet Mu?ammad, and who by this time had gained significant influence as a social class based on their charismatic descent. While other dynastic powers fostered relationships with various members of the ashrāf, the Fā?imid–ashrāf dynamics were distinctive in that the Fā?imids legitimised their rule as Ismā?īlī Shī?ī imām-caliphs, based on their claim of descent from the Prophet Mu?ammad, and as the sole successors to his authority and leadership over the Islamic world. Consequently, Fā?imid–ashrāf relations were permeated by fraternal camaraderie as well as by competing contestations based on their shared claim to Prophetic lineage.     

传导系统中TE/TM偏振模态的辨析

电磁波/光传输特性研究中常用TE/TM偏振描述波的传播模式.不同系统中对TE/TM偏振的定义却存在差别,有时还与s/p态混合使用,使研究者在理论和实验研究中产生概念混淆和结果矛盾的问题.本文从电磁理论出发,对传导系统中可能存在的偏振模式进行深入的推导和分析,解释TE/TM偏振态存在原因和可能状态,对其与s/p态的区别进行比较说明.针对空间偏振态的易混淆问题进行逐一解答.     

Laboratory medicine in pandemic of COVID-19

After the outbreak in China in the year 2019, severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) quickly spread around the world causing a protracted pandemic. Approximately one-third of infections appear to be asymptomatic. Symptomatic disease is characterized primarily by symptoms of respiratory tract infection of varying severity. But Coronavirus Disease 2019 (COVID-19) is much more than an acute respiratory disease because SARS-CoV-2 affects many organs inducing a vast number of symptoms such as cardiovascular, neurological, gastrointestinal, dermatological, with numerous complications. Short and long-term effects of infection, severe ones, and especially mild forms of the disease which affect a huge number of patients need to be further investigated. Laboratory medicine has a crucial role in early diagnosis of the disease, recognition of the patients who need hospital care, and close monitoring of hospitalized patients to timely identify associated clinical complications as well as follow-up of patients with long-term COVID-19.     

基于FPGA技术的8B/10B信道编码设计

该文在分析8B/10B编码原理的基础上,设计了改进型8B/10B编码方案.该编码在完成高速数据信道编码功能的同时,可另外提供一路实时低速信道.基于FPGA技术采用查表法实现了改进型编解码电路,通过仿真验证了该编码方案的有效性,并在Spartan6系列FPGA中进行了综合测试.结果表明,此编解码方法占用的FPGA逻辑资源...     

基于工业以太网的高炉监测系统

基于工业以太网的高炉监测系统,介绍了Modbus/TCP通信协议,以及由工控机、工业以太网、ADAM5000/TCP数据采集模块和传感变送器等组成的远程数据采集系统的构建,并通过编写VB6.0应用程序实时监测高炉生产工艺参数.     

最后航次法律问题比较研究

最后航次问题是定期租船中一个常见的问题。英国、美国对最后航次的相关问题,包括还船日期的确定、合法航次与非法航次之分、承租人的赔偿等,都有完整的法律规定。我国在海商法中的相关规定较为模糊,存在合同法及海事实践的衔接问题,对出租人较为不利。     

专题数据库光盘发布技术原理及其实践应用

当前信息服务机构已纷纷通过建立专题数据库为用户提供信息服务,而专题数据库的发布、使用方式则在很大程度上影响专题数据库的使用效果。首先讨论数据库的一般发布载体,主要有纸张I、nternet和光盘等;其次讨论了专题数据库光盘发布实现的技术、原理;最后给出了此技术的应用、实践实例。