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21.
The high mobility group (HMG) proteins I and Y are well characterised non-histone chromosomal proteins which bind to A-T rich
regions of DNA and regulate gene expression and/or DNA replication. A correlation has been demonstrated between the increased
expression of HMG-Y proteins and malignancy. However, it is not known whether the expression of HMGs particularly, the Y group,
is a function of proliferation rate. In the present study, we have used normal tissues of calf testes, thymus and liver. The
results show distinctly high expression of HMG-Y proteins in testes than in thymus and the expression was practically undetectable
in liver. The results suggest that even in normal tissues there is a direct correlation between the proliferation rate and
the expression of the HMG-Y proteins, which can partly explain its increased expression in cancer. 相似文献
22.
AIM To filtrate breast cancer resistance protein(BCRP)-mediated resistance agents and investigate the mechanism,so as to provide valuable datum for optimization clinical chemotherapy scheme to tumor with evaluation marker of BCRP expression. METHODS MTT assay was used to filtrate BCRP-mediated resistance agents with PA317/Tet-on/TRE-BCRP cell of different expression levels of BCRP after treated with different concentration anticancer agents. High performance liquid chromatography(HPLC) was applied to measure relative dose of intracellular retention resistance agents. Nuclear DNA fluorescence dye,Hochest 33258, staining and flow cytometry were adopted to detect apoptotic cells after treated with drugs. RESULTS There were shown increasing durg-resistance to 5-fluorouracil,methotrexate, doxirubicin, pirarubicin,etoposide and mitoxantrone followed with increasing expression of BCRP on PA317/Tet-on/TRE-BCRP cells(P<0.05, n=3),but shown sensitive to paclitaxel, cisplatin, vincristine, mitomycin and vindesine. There also was shown significant negative correlation between the intracellular retention dose of 5-fluorouracil with different expression of BCRP(r=-0.885, P<0.05, n=3).There were shown parallel results of that decreasing cellular apoptotic rate with increasing cellular expression of BCRP after treated with 5-fluorouracil by fluorescence dye staining and flow cytometry(P<0.05, n=3),and also shown significate rise of the apoptotic rate of BCRP expression cells after treated with Ko143 (P<0.05, n=3). Every group of cells could be different extently blocked in phase of G0/G1 treated with 5-fluorouracil. CONCLUSION Resistance of 5-fluorouracil could be especially mediated by conjugated with BCRP and acted as drug exclude-pump substrate. Cellular ability resistant to 5-fluorouracil-induced apoptosis could be reinforced by BCRP expression. 相似文献
23.
24.
[目的]了解张掖市适龄女性对乳腺健康知识及乳腺癌的认知情况和乳腺自检现状,探讨其影响因素,为乳腺健康教育的实施及提高主动预防意识提供可行性依据。[方法]抽取张掖市五县一区18—65岁当地妇女1200人以问卷调查的方式进行乳腺健康知识、乳腺癌相关知识的调查,应答率99.6%。[结果]在调查的1195人中,调查对象对乳腺健康知识与乳腺癌概念混淆,对乳腺健康知识与乳腺癌认知途径主要来源于电视与社会教育,对乳腺癌认知较清楚的更是基于周边有乳腺癌患者的基础上;对乳腺癌的危险因素认知,城乡差距显著;乳腺癌治疗现状及生存状态认知不够。[结论]张掖市妇女对乳腺健康知识与乳腺癌的相关知识认识不足,对乳腺癌的治疗信心偏低,乳腺健康知识与乳腺癌认知途径单一、信息缺乏,应通过多元化健康教育加强妇女对乳腺健康知识的了解,提高妇女乳腺健康知识与癌的认知水平,加入疾病主动预防的健康队伍中来。 相似文献
25.
INTRODUCTION In cancer patients, tumor immunity is generally impaired. However, tumor immunity may occur in-vivo as circulating tumor-specific antibodies, with cytotoxic T lymphocytes (CTLs) having been identi- fied in cancer patients (Disis et al., 1997; Albert et al., 1998) suggesting that effective anti-tumor responses may be elicited through immunotherapeutic strategies. In recent years, a number of clinical trials have proved the safety and feasibility of using human dendritic cell… 相似文献
26.
目的:探讨金属硫蛋白(MT)在乳腺癌中表达的状况.方法:采用免疫组化S-P方法.检测57例乳腺癌中MT表达的情况.结果:MT在乳腺癌组织中的总阳性率为59.65%.浸润性导管癌、单纯癌和髓样癌中MT的阳性率分别是69.05%、80.00%、10.00%,髓样癌和其他组有显著性差异(P<0.01).MT的表达与淋巴是否转移相关性不明显.结论:检测MT为制定化疗方案提供依据. 相似文献
27.
目的:研究热休克蛋白27(Heat Shock Protein27)在乳腺癌中的表达.方法:采用免疫组化方法检测48例乳腺癌组织中的HSP27.结果:HSP27在乳腺癌中阳性表达率为54.17%(26/48).在非浸润性癌和浸润性癌中阳性表达率为75.00%(3/4)和52.27%(23/44).HSP27阳性表达在非浸润性癌和浸润性癌中无明显差别(P>0.05).HSP27阳性表达与有无淋巴结转移无关(P>0.05).结论:在乳腺癌中HSP27有较高表达,检测乳腺癌中HSP27对化疗有指导作用. 相似文献
28.
R. Nalini C. R. Wilma Delphine Silvia Purnima M. Makhija Sheila Uthappa 《Indian journal of clinical biochemistry : IJCB》2005,20(1):165-168
Prognostic factors influence the modality and course of therapy in breast cancer. These include both histopathological and
biochemical variables. This study was aimed to find out if any correlation exists between the biochemical tumor marker-serum
CA 15.3, and the lymph node staging of Nottingham’s Prognostic Index (NPI). It was observed that serum CA 15.3 showed a statistically
significant correlation with lymph node staging of NPI. Therefore it is concluded that CA 15.3 can be utilized as a useful
prognostic marker, particularly in cases where histopathological variables such as tumor size and grade have been altered
by pre-operative chemotherapy. 相似文献
29.
Nicola Brown Jennifer White Amanda Brasher Joanna Scurr 《Journal of sports sciences》2013,31(9):801-809
AbstractAlthough it is acknowledged that appropriate breast support during exercise is important, no published literature has assessed breast support usage in a cohort of female marathon runners. This study aimed to identify sport bra use and perceived importance of sports bra use in female marathon runners. Bra satisfaction, incidence of bra related issues and factors that influence the appropriateness of sports bras were also investigated. A 4-part, 30-question survey was administered to 1397 female runners at the 2012 London marathon registration and via an online survey. In total 1285 surveys were completed. Sports bra use and its perceived importance was high, however was lower in moderate compared to vigorous activity, and lower in participants with smaller breasts. Seventy-five per cent of participants reported bra fit issues. The most common issues were chaffing and shoulder straps digging in, with a higher incidence of issues reported by participants with larger breasts. Use of professional bra fitting was low, and perceived knowledge of breast health was poor. Engagement with sports bra use is high although sports bra design could be improved to alleviate bra fit issues experienced by female runners. Educational initiatives are needed to ensure females are informed regarding the importance of breast support and appropriate bra fit during activity. 相似文献
30.
Sang-sang REN Jing-wen DENG Meng HONG Yan-li REN Hai-jing FU Yan-ning LIU Zhi CHEN 《Journal of Zhejiang University. Science. B》2019,(1):23-31
本文旨在对细胞免疫治疗相关的伦理学问题进行探讨,并通过分析我国细胞免疫治疗相关伦理学实践及现状,为规范我国肿瘤细胞免疫治疗的伦理标准提供参考。随着免疫学、分子生物学等学科的飞速发展和高通量测序等技术的普及,肿瘤免疫治疗已成为第四大肿瘤治疗方法。肿瘤细胞免疫治疗,如各种基于T细胞、自然杀伤(NK)细胞和树突状细胞(DC)的免疫治疗在其中发挥了重要作用。随着临床实践的开展,一些相关的伦理学问题逐渐暴露出来。如何在治疗过程中坚持基本伦理原则,有效指导并解决相关临床问题,提高治疗效果,保护受试者权益,是目前亟需研究的重要课题。本文对与之相关的伦理学问题进行探讨,并通过分析我国细胞免疫治疗相关伦理学实践及现状,以期为规范我国肿瘤细胞免疫治疗的伦理标准提供参考。 相似文献