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71.
针对现有DNA教具缺陷,分析DNA螺旋结构特征,提出一种DNA双螺旋结构动态演示仪。运用3D打印技术制作形状特殊的零部件,引入特殊的尼龙线配合球头副辅助DNA运动,最终制作出DNA双螺旋结构动态演示仪。经实际授课使用,证明该演示仪能够辅助提高教学效果。 相似文献
72.
Wang AR Lin WW Chen XT Lu GD Zhou J Wang ZH 《Journal of Zhejiang University. Science. B》2008,9(10):818-822
A new stem rot disease is found to occur naturally on Arabidopsis plants in greenhouses of Fuzhou, China. In order to identify its pathogen, we conducted a series of fimgal isolation and purification, plant reinoculation, and ascus and ascospore induction from the sclerotia. The isolate caused typical water-soaked lesions after reinoeulation and produced sclerotia both on Arabidopsis plants and culture medium plates, and the sclerotia could be induced to produce discal apothecia and 8 binucleate ascospores per ascus. These disease symptom and fungal morphology data revealed that the fungus Sclerotinia sclerotiorum (Lib.) de Bary was the pathogen for Arabidopsis stem rot. To confirm this, we further amplified its large subunit ribosomal DNA (LSU rDNA) by polymerase chain reaction (PCR), and compared the sequence with the known LSU rDNA sequences in GenBank. The results show that the sequence shares the highest identities with the LSU rDNAs of different S. sclerotiorum strains. Taking all these data together, we concluded that the fungus that caused the Arabidopsis stem rot is S. sclerotiorum (Lib.) de Bary. This is the first report that Arabidopsis is naturally infected by S. sclerotiorum. 相似文献
73.
A parallel architecture for efficient hardware implementation of Rivest Shamir Adleman(RSA) cryptography is proposed.Residue number system(RNS) is introduced to realize high parallelism,thus all the elements under the same base are independent of each other and can be computed in parallel.Moreover,a simple and fast base transformation is used to achieve RNS Montgomery modular multiplication algorithm,which facilitates hardware implementation.Based on transport triggered architecture(TTA),the proposed architecture is designed to evaluate the performance and feasibility of the algorithm.With these optimizations,a decryption rate of 106 kbps can be achieved for 1 024-b RSA at the frequency of 100 MHz. 相似文献
74.
基于证书的签名方案能够很好地的解决传统PKI中的密钥托管问题。基于一个基本的证书签名方案,进行了基于证书PKI/CA的研究,同时对其框架和类进行了详细的设计。最后给出了实验仿真结果,结果表明该框架能够有效的应用于基于证书公钥密码体制。并且实验结论正确。 相似文献
75.
76.
Ines Ben Chobba Amine Elleuch Imen Ayadi Lamia Khannous Ahmed Namsi Frederique Cerqueira Noureddine Drira Néji Gharsallah Tatiana Vallaeys 《Journal of Zhejiang University. Science. B》2013,14(12):1084-1099
Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various troublesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues (roots and leaves) of Tunisian date palm trees (Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar (PDA) medium and identified by a sequence match search wherein their 18S–28S internal transcribed spacer (ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fell into two groups, namely Nectriaceae and Pleosporaceae. Additionally, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. Restriction fragment length polymorphism (RFLP) analysis of the ITS from 200 fungal clones (leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiella (Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria (members of the Nectriaceae family), the leaves were essentially colonized by Alternaria (members of the Pleosporaceae family). Overall, the findings of the present study constitute, to the authors’ knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees (P. dactylifera). 相似文献
77.
DNA序列的数形表示是用数学方法和计算机处理方法分析生物分子序列首先要解决的问题。序列表示的优劣会对最终分析结果有直接的影响。本文介绍了现有几种DNA序列数形表示方法。 相似文献
78.
通过线性分段将连续性的金融时间序列转化为离散性的字符序列,并基于DNA序列分析法,讨论了此类字符序列的标度特性,以及在金融数据时间序列预测中的可能应用. 相似文献
79.
P. Sharma M. Bose Isa Mohd S. Bagdi H. G. Raj 《Indian journal of clinical biochemistry : IJCB》2000,15(2):83-87
Genomic DNA from a clinical isolate ofMycobacterium avium-intracellulare complex was purified and cloned in PBR 322 at the tetracycline resistance site using Bam HI restriction enzyme. A 16 kb cloned
fragment was purified, radiolabeled and used as a probe. Genomic DNA isolated from nineteen MAC strains, threeM. tuberculosis strains and oneM. kansasii strain were digested with Eco RI restriction enzyme, Southern blotted and hybridized with the 16 kb cloned and labeled fragment.
Twelve MAC strains showed positive hybridization although five strains gave faint signals. Positive hybridization was noted
in two out of the threeM. tuberculosis strains, possibly due to shared DNA homology. No signal was received from the singleM. kansasii strain used in this study. 相似文献
80.
金融企业通过使用电子银行/支付安全系统(DNA)技术后可以把交易资料和授权资料分两个渠道传送到银行账户主机。DNA系统改变了银行各种电子交易处理平台沿用几十年的单轨式交易传送系统,而采用双轨式的交易传送系统,有效解决了因客户重要金融资料遗失或被盗后引发的风险。本文以一商业银行对此技术的应用及实现进行阐述。 相似文献