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排序方式: 共有499条查询结果,搜索用时 15 毫秒
81.
利用乳糖诱导重组大肠杆菌BL21(DE3)中Pfu DNA聚合酶基因的表达。对诱导菌株起始生长量、乳糖浓度和诱导持续时间进行了优化,尝试使用JK110弱阳离子交换柱和Sephadex G-75凝胶层析柱来分离纯化Pfu DNA聚合酶,并用PCR扩增实验检测酶活性。结果表明乳糖可以有效诱导Pfu酶的表达,JK110离子交换柱有较好的纯化效果,从500 mL培养液中可以得到3×105U的酶活,比活达22,200 U/mg,结果好于IPTG诱导表达。  相似文献   
82.
DNA分子标记技术在水生动物遗传多样性研究中的应用   总被引:1,自引:0,他引:1  
简要阐述了几种常见DNA分子标记技术,如限制性片断长度多态性(restriction fragment length polymorphism,RFLP)、随机扩增多态性DNA(random amplified polymorphie DNA,RAPD)、扩增片断长度多态性(amplified fragment length polymorphism,AFLP)、简单重复序列或微卫星标记(simpie sequencerepeat,SSR)、内部简单重复序列(inter-simple sequence repeat,ISSR)和单核苷酸多态性(single nucleotidepelymorphisms,SNP)等的基本原理、技术上的优缺点及其在水生动物遗传多样性研究中的应用,包括遗传多样性的鉴定、品种亲缘关系与分类的研究和种质资源的鉴定等.最后,对DNA分子标记技术在水生动物遗传多样性应用中的存在问题进行了简单的讨论.  相似文献   
83.
Lymphocyte dysregulation in coronavirus disease-19 (COVID-19) is a major contributing factor linked to disease severity and mortality. Apoptosis results in the accumulation of cell-free DNA (cfDNA) in circulation. COVID-19 has a heterogeneous clinical course. The role of cfDNA levels was studied to assess the severity and outcome of COVID-19 patients and correlated with other laboratory parameters. The current case series included 100 patients with mild COVID-19 (MCOV-19) and 106 patients with severe COVID-19 (SCOV-19). Plasma cfDNA levels were quantified using SYBR green quantitative real-time PCR through amplification of the β-actin gene. CfDNA level was significantly higher in SCOV-19 at 706.7 ng/ml (522.6–1258) as compared to MCOV-19 at 219.8 ng/ml (167.7–299.6). The cfDNA levels were significantly higher in non-survivor than in survivors (p = 0.0001). CfDNA showed a significant correlation with NLR, ferritin, LDH, procalcitonin, and IL-6. The diagnostic sensitivity and specificity of cfDNA in the discrimination of SCOV-19 from MCOV-19 were 90.57% & 80%, respectively. CfDNA showed a sensitivity of 94.74% in the differentiation of non-survivors from survivors. CfDNA levels showed a significant positive correlation with other laboratory and inflammatory markers of COVID-19. CfDNA levels, NLR, and other parameters may be used to stratify and monitor COVID-19 patients and predict mortality. CfDNA may be used to predict COVID-19 severity with higher diagnostic sensitivity.  相似文献   
84.
ABSTRACT

This study aimed to systematically review the association between cardiorespiratory fitness and telomere length (TL). Studies were identified from searches in Cochrane Central, PubMed, Scopus, Sportdiscus, and Web of Science databases through July 2019. Eligibility criteria included: cross-sectional, prospective, and experimental study design; outcomes included TL; results expressed the relationship between cardiorespiratory fitness and TL; studies published in English, Portuguese, or Spanish. A total of 20 articles met the inclusion criteria. Sixteen studies (80%) reported a significant relationship between cardiorespiratory fitness, or training load, and TL. Better cardiorespiratory fitness or a large cardiorespiratory training load are associated with an increase in TL. Although, TL was related to regular moderate-to-vigorous aerobic exercise and cardiorespiratory fitness in older healthy humans, it was not related to cardiorespiratory fitness among young subjects. There seems to be a positive and significant relationship between cardiorespiratory fitness and TL, mainly among middle age and older people, which emphasizes the importance of cardiorespiratory fitness for healthy ageing. Therefore, endurance exercise and better cardiorespiratory fitness may regulate the TL in middle age and older adults, slowing the cellular ageing process.  相似文献   
85.
INTRODUCTION Genetic diversity is a major issue of conserva-tion biology recognized by the IUCN (Frankham etal., 2002). Within a population it reflects the evolu-tionary potential to adapt to novel environmentalchanges. Therefore, during the past few years, thegenetic diversity of many threatened mammals, birds,fish, insects and plants have been investigated(Frankham et al., 2002). As a direct and indirectconsequence of human actions, more and more spe-cies or populations are faci…  相似文献   
86.
本文全面考虑了DNA的碱基对的相互作用、双链的整体振动效应和链内分子的集体激发等运动状态,应用在我们最近在蛋白质分子中建立的新理论与其它方法,研究了DNA分子的动力学特性,集体激发与孤立子运动.并应用此结果深入研究DNA的一些生物功能,如复制、转录等问题.  相似文献   
87.
要刊速递     
《天津教育》2011,(7):2-2
韩国与中国一样,都是深受儒家伦理影响的东方国家,有重视教育的传统。类似“再苦不能苦孩子、再穷不能穷教育”的观念,在韩国比在中国贯彻得更彻底、更极端。“学历是门面,是进入上流社会的通行证”,这样的观念深入韩国社会的DNA。  相似文献   
88.
随着移动设备和无线设备的大量使用,需要一种新的公钥密码方案,来适应这些设备在计算能力和带宽方面的限制,同时要提供足够级别的安全性。讨论了椭圆曲线密码系统在这种受限环境中的使用和它的安全性的基础,给出了椭圆曲线密码系统的加解密和数字签名算法,探讨了椭圆曲线密码的安全性,最后概括了椭圆曲线密码系统的研究和应用现状。  相似文献   
89.
The precise etiology and reasons for the increase in incidence of autoimmune disorders still remain unclear, and although both genetic and environmental factors have been proven to shape individual predisposition, it is not known which of the factors, if not both, is responsible for the boom observed during the last decades. In order to establish whether a higher frequency of autoimmune-predisposing alleles may explain this increase we took advantage of ancient DNA methodology to establish the genetic predisposition, conferred by cytotoxic T lymphocyte associated antigen-4 (CTLA4) +49A/G and human leukocyte antigens (HLA) DQBI^57, in population inhabiting Poland in the Middle Ages. After successful typing of 42 individuals from a 12th-14th's century archeological burial site, we found that frequencies of the predisposing alleles in the medieval population were higher than they are at present, suggesting thus that the recently observed incidence increase results most probably from factors of other than genetic nature.  相似文献   
90.
Objective: To identify the mutations of iduronate-2-sulfatase (IDS) gene, to reveal its mutation features, and to establish a basis for genetic counseling and prenatal gene diagnosis of Hunter syndrome. Methods: Urine glycosaminoglycans (GAGs) assay, PCR and DNA sequencing were performed to detect mutation of IDS gene of the patient and his parents. Results: The result showed that the patient was: DS( ), HS( ), KS(-), CS(-), and that both of his parents were negative. A frame-shift deletion mutation (1062 del 16) was identified in exon 7 of the patient's IDS gene. His parents' genotypes were normal. Conclusion:The patient's mutation was not inherited by his parents but a novel one. The mutation probably altered the primary structure and tertiary structure of IDS enzyme protein remarkably and lowered the activity of IDS enzyme greatly. Therefore it is supposed to be the direct cause of the disorder.  相似文献   
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