有限域中的循环矩阵在密码学方面的相关问题

利用有限域上循环矩阵的性质,使用2种不同方法去解决有限域上可逆循环矩阵的个数问题. 最后给出有限域上可逆循环矩阵个数的计算公式,并对多变量密码学中的循环矩阵的应用进行简要分析, 这对矩阵理论研究和相关密码学的分析有促进作用.     

骨骼肌基因转移系统的建立

以EGFP为标记基因，利用重新构建的pAV-EGFP质粒，采用直接注射方法，进行了离体和在体基因转移研究。实验结果显示，经脂质体包裹后，质粒可以有效地进行细胞转移；DNA表达载体肌肉直接注射后，利用共聚焦显微镜可以在注射点周围的肌细胞观察到绿色荧光蛋白；而在对侧肢PCR和共聚焦显微镜的结果均为阴性。实验结果表明，肌肉直接注射DNA可以在肌肉得到表达，将会为治疗肌肉损伤带来新的方法。     

蝴蝶不同部位提取DNA效果的研究

提取高质量的蝴蝶基因组是蝶类分子系统学研究的重要步骤。本文通过对蝴蝶干标本不同部位DNA提取效果的比较,找出了最适合蝴蝶干标本基因组DNA提取的部位,同时用PCR扩增的方法进行了检验,力争为开展蝴蝶的分子生物学研究奠定基础。结果表明,足部的提取效果最佳。     

Statistical properties of nucleotide clusters in DNA sequences

Using the complete genome of Plasmodiumfalciparum 3D7 which has 14 chromosomes as an example, we have examined the distribution functions for the amount of C or G and A or T consecutively and non-overlapping blocks of m bases in this system. The function P(S) about the number of the consecutive C-G or A-T content cluster conforms to the relation P(S)oce-as;values of the scaling exponent αCG are much larger than αAT; and αAT of 14 chromosomes are hardly changed, whereas αCG of 14chromosomes have a number of fluctuations. We found maximum value of A-T cluster size is much larger than C-G, which implies the existence of large A-T cluster. Our study of the width function ξ(m) of cluster C-G content showed that follows good power law ξ(m)ocm-γ. The average γ for 14 chromosomes is 0.931. These investigations provide some insight into the nucleotide clusters of DNA sequences, and help us understand other properties of DNA sequences.     

激光对DNA分子的非线性作用效应的混沌行为分析

本文对第一作者提出的激光－ＤＮＡ相互作用方程的非线性混沌行为进行了解析分析和数值分析，将有助于对激光的生物遗传诱变效应的解释．     

mtDNA analysis of the human remains buried in the sarcophagus of Federico II

The sarcophagus containing the remains of Federico II, located in the Cathedral of Palermo (Sicily, Italy), was opened on 1998 to perform a multidisciplinary survey [1]. Next to the remains of Federico II and in close contact with them were laying two other skeletons belonging, according to historical records, to Pietro II di Aragona and to an anonymous person (“The Third Individual”), probably a woman. The bones appeared severely deteriorated. Chemical analysis performed on bone samples excluded that the bodies underwent some kind of embalming process. The analysis of mtDNA from bone samples taken from the three skeletons was successful in only one of the two labs involved. The HVR1-mtDNA sequence (region: from nt 16,035 to nt 16,395), obtained from the bone samples of Federico II and “The Third Individual” appear identical but bear double peaks at the same nucleotide positions, suggesting mixing (i.e. contamination) of different mtDNA types. The HVR1 sequence obtained from the bone sample of Pietro II di Aragona does not present double peaks and differ from the Cambridge Reference Sequence (CRS) at six nucleotide positions. Cloning experiment of the Federico II amplicon demonstrated that the mixed mtDNA types are only two: one identical to CRS, the other identical to the sequence of Pietro II di Aragona. A reconstruction of these data are proposed in the Discussion. Due to the problematic context in which this study was carried out (mixed and deteriorated biological material, failure to replicate results in two different labs), our results and reconstruction can only be offered on a tentative basis. It is hoped that the data presented in this study will reveal useful, for future comparison, if further molecular genetics research will be carried out on the royal dynasties that ruled Sicily in the early centuries of the past millennium.     

基于RT-HPLC的古代mtDNA序列差异性分析

线粒体DNA具有突变速度快、重组率低、严格母系遗传、拷贝数多等特点,近年来成为分子考古研究中的一个热点。为了研究古墓群中的血亲关系,可以从古墓葬遗留骨骸、牙齿中提取和扩增mtDNA片断进行分析。通过设计2对部分重叠的引物,扩增线粒体16055-16218区域目标片段,进行反相高效液相色谱分析,确认四个样品对应片断在反相柱上的保留时间上存在差异,以此推断扩增片段在序列上可能存在差异。为利用mtDNA分析人类母系血缘关系奠定了基础。     

The Plasma DNA Concentration as a Potential Breast Cancer Screening Marker

Increased levels of plasma DNA have frequently been noticed in the blood plasma of cancer patients. The possibility of using plasma DNA level as the indicator of tumor stage in breast cancer was investigated in plasma samples obtained from 100 breast cancer patients and 100 healthy women who were included as controls. Circulatory plasma free DNA was extracted from plasma samples and quantified by fluorometer. The median concentration of plasma DNA in the plasma samples from breast cancer patients classified by TNM staging system as stage I, II, III, IV and breast surgical patients were 0.5, 235, 422, 1,280 and 0.5 ng/ml, respectively. The level of plasma DNA in the stage II- IV group was significantly higher than those in the surgical group with breast cancer and control group (P value < 0.001). The plasma DNA concentration in stage II, III and IV of breast cancer were higher when compared with healthy group. These tumor size, TNM stage and metastasis were significantly correlated with plasma DNA. The cut point of 120 ng/ml was early screening and treatment follow up breast cancer.     

细胞计算进展与展望

细胞是生物体最基本的结构和功能单元,它蕴含了大自然几千万年进化所沉淀的智能。细胞外膜将细胞内部和细胞环境分割开,控制细胞内外物质的进出,而细胞内膜将细胞内部分成具有不同生物功能的细胞器,这使得细胞自身构成了一个分布式并行信息处理系统。文章介绍了研究细胞计算的背景,细胞的基本结构和功能,以及基于细胞的结构和功能而发展起来的计算机科学新领域:膜计算。膜计算目前主要有3类计算模型:细胞型计算模型、组织型计算模型和神经型计算模型,这3类计算模型分别以单个细胞、细胞群体和神经元作为计算载体。膜计算在生物系统建模等方面具有重要的应用价值。随着生物技术的发展,人们用大肠杆菌等实现了部分膜计算模型。最后,展望了膜计算在生物学、医学、大规模数据存储、大规模并行计算等方面的应用前景。