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胡轶群 《和田师范专科学校学报》2013,(4):80-83
笔者按照文稿准备到表达的过程,由浅至深地从播音创作基础、朗读学、表演学、艺术接受学、教育学等角度,提出了聆听过程中的三个阶段和应该注意的问题,具体分析了《出师表》的历史背景、写作背景和播音背景,并对于舞台表演时的状态给予了提示。希望对于中学老师进行语文教学和朗诵辅导时提供一些思路。 相似文献
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过去的二十几年,我国环境建设不断升温,各类环境小品越来越多地出现在公众视野。该文以两个引发争议的环境小品案例分析了目前环境小品设计存在的创意和表达问题.并分别就这两方面做出一些思考探讨。 相似文献
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朱柳敏 《辽宁教育行政学院学报》2008,25(7)
口语表达能力是中国学生一直久攻不下的难题.通过教学,帮助学生提高用英语进行交际的能力是摆在广大英语教师面前的一个迫切需要解决的课题.英语口语教学可以从神经生物学的角度分析情感在外语学习过程中的作用和影响,从神经语言学的角度具体分析影响英语口语表达的因素,并以此为基础进行以需求为目的分层次的英语口语教学. 相似文献
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目的:在康脑液方剂干预下观察皮质区内源性神经细胞干细胞因子(SCF)mRNA在大鼠脑缺血再灌注损伤后不同时间的表达情况。方法:成年SD大鼠,以线栓法建立大脑中动脉缺血再灌注模型,随机分为模型组、药物干预组和假手术组。原位杂交技术检测脑缺血1.5h再灌注1~14d后,脑皮质区SCFmRNA表达情况。结果:脑缺血再灌注后,药物干预组、模型组SCFmRNA的表达在皮质区均明显高于假手术组,于第7天达高峰,第14天下降。结论:药物干预后脑缺血再灌注脑皮质区SCFmRNA表达在不同时间点与模型组具备相同的表达规律,两组SCFmRNA的表达无显著性差异。 相似文献
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如何充分掌握利用委婉的表达是个很复杂的问题,启用何种委婉的说法会因人因时因地等不同的环境或条件而有所不同。作为一种修辞手段,委婉语还不仅仅是所谓“捡好听的说”,它与语言学中关于如何判定词义褒贬不无关系。实际上没有一种委婉的说法不打上交际者个人交际能力的烙印。本文重点讲了生活中常见的几种委婉的表达,也是外语教师教学中常常忽略的问题。 相似文献
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大学生非理性行为探析 总被引:4,自引:0,他引:4
处于青年中期大学生 ,受生理和社会经济环境的影响 ,在社会交往中容易产生非理性行为。本文拟就大学生非理性行为的表现、原因及矫正大学生非理性行为的对策作一初浅探析 ,以引导大学生理智克服非理性行为 相似文献
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Sun Jie Huang He Zhu Yuan-yuan Lan Jian-ping Li Jing-yuan Lai Xiao-yu Yu Jian 《Journal of Zhejiang University. Science. B》2005,6(12):1141-1147
Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes
to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology
information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic
leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification
(TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells
(0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease.
Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science
Foundation of China (No. 39870339) 相似文献