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Feng-ying LI Xiao-bin REN En-ping XU Qiong HUANG Hong-qiang SHENG Bing-jian LV Mao-de LAI 《Journal of Zhejiang University. Science. B》2010,11(4):258-266
Regenerating gene IV (RegIV),a member of the regenerating gene family discovered in 2001,has been found to be involved in malignancy in several different organs including the stomach,colorectum,pancreas and prostate,but the overall expression profile of RegIV has not been reported. To learn more about RegIV,we evaluated its distribution by immunohistochemistry (IHC) in a total of 360 samples including 24 types of normal tissue,40 benign and malignant lesions,and 18 neuroendocrine tumors. We found that in no... 相似文献
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Sun HC Xue FM Qian K Fang HX Qiu HL Zhang XY Yin ZH 《Journal of Zhejiang University. Science. B》2006,7(4):324-330
To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400μg of the p215C3LYZ vector, over 2.0 μg/ml ofrhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhL YZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hL YZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis. 相似文献
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作为唐代清乐主流的清商曲辞,其比重不如相和、杂曲;作为唐代清商曲主流的三十二调,其歌辞比重也不如清商曲辞中的其他曲调.这两"少"说明衰落了的音乐在一定程度上对歌辞的创作具有抑制作用.娱乐性的歌辞表达的往往是一种泛化的情感,这并不利于作者情感的抒发.娱乐性的减弱使唐代诗人得以借乐府古题来抒发其强烈的感情. 相似文献
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研究讨论全球化背景下少数民族地区的自我表达问题,从红河哈尼梯田存在大量英语翻译错误入手,实地考察和大量走访表明该地区的自我表达已自成体系。自我表达的内容被划分为身份、定位和价值三个层面。互动中的当地人、管理层和外来者三方都进行着"意义"生产,三方共同努力构建了表达系统。对异同关系的再思考、深挖民族文化内涵、彰显民族平等是未来表达的方向。 相似文献
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不完全表述是语言表达中的常见现象,文章以影视文学作品为素材,主要从存在性前提、逻辑前提、常识性前提三个方面进行探讨,并证实了从前提理解不完全表述方法的可行性。因此,作为一种新的尝试,对交流过程中谈话双方信息的理解,有一定的实践意义。 相似文献
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邱冬梅 《毕节师范高等专科学校学报》2014,(1):7-14
定指性强是“x-方的N”结构具有照应功能的重要前提。该类结构在篇章中的照应表现为回指和下指两类。从内部看,该结构的构件在用词和组配方面表现出明显的倾向性。从外部看,是否选择“x·方的N”结构作照应语,受到语言和非语言语境因素的制约。 相似文献
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清代民法为任何一个对法制史有所涉猎的人所熟悉,但人们大都是从清代官方的表达中对它加以了解的.美国学者黄宗智在<清代法律、社会与文化:民法的表达与实践>一书中,从几个方面论证了清代法律制度的实际运作与清代官方表达之间有很大的差异,即实践与表达的背离.这使读者们不得不努力追忆以往所读过的清代官方表达与前人著说,与黄先生的观点做一番对照,以探求历史的真迹. 相似文献
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石坚平 《历史教学(高校版)》2011,(1)
契约文书文类的特殊性决定了契约文书中的货币表达方式必须同时兼顾国家货币制度、民间社会实践与地方财税政策三者的关系。面对国家制度的僵化迟滞,地方官府的被动适应,广府侨乡社会一面广泛使用银元,主动选择采用银元制的流通方式,以顺应中国货币制度近代化的客观趋势;另一方面不断创新契约文书的货币表达方式,创造出一个系列介于新、旧货币制度之间的过渡性混合制的货币表达方式,既维护了王朝国家的制度性规定,又为广府侨乡社会自主选择货币流通方式提供了灵活的弹性选择空间。 相似文献