急性运动对机体免疫系统的影响

运用文献综述的研究方法,对急性运动对机体免疫系统的影响进行了分析,讨论了它们的发生机制和可能的生理或病理意义。     

细胞凋亡与运动训练关系的研究进展

细胞凋亡和坏死是截然不同的细胞死亡形式。目前 ,细胞凋亡的研究已涉及生命科学的各个领域。在运动训练状态下开展有关细胞凋亡机制的研究 ,可从新的角度解释疲劳的发生机制 ,找出监测疲劳的更有效指标 ,并对开发新的促进恢复的营养补剂和全民健身产生积极作用     

SRB显色法用于抗癌药物敏感性试验的研究

采用ＳＲＢ显色法对９６孔培养板中短期培养的人大肠癌细胞株Ｃｏｌｏ２０５、人骨肉瘤细胞株ＯＳ７３２,人舌癌细胞株Ｔｃａ８１１３经５－ＦＵ,顺铂,蟾酥等药物处理后的活性进行了研究。结果表明,ＳＲＢ法对这些细胞株的体外抗癌药物敏感试验结果稳定,该法与其它细胞毒试验相比有一定优点,值得推广应用。     

运动训练中内皮素与心血管系统关系的综述

为了了解运动训练中内皮素对心血管系统的作用.采用文献资料法论述了内皮素的主要生理作用,综述了国内外有关内皮素与运动训练关系的研究现状及该领域研究中存在的问题.研究表明:适宜强度的有氧耐力运动,可以改善内皮素的释放量进而有利于心血管系统在运动训练中的适应.     

免疫细胞分离鉴定综合实验设计

在传统免疫细胞分离检测实验教学的基础上,利用高性能正置荧光显微镜和流式细胞仪以不同技术检测鉴定免疫细胞,对实验教学内容和教学方法进行了改革和实践.将教学与临床科研相结合,利用大型贵重仪器以及合理安排实验时间开发综合实验.结果表明,学生不仅可以更有效消化医学免疫学课程基础知识,了解学科新技术的发展,而且可以培养学生的科研...     

流动注射微型电化学发光池的研制及性能研究

报道了一种自行设计和研制的新型流动注射电化学发光（EcL）池,并对该流动注射电化学发光池的结构和性能进行了优化,在发光试剂鲁米诺浓度5×10^-10mol／L-5×10^-8mol／L范围内,检测池ECL强度随浓度呈现良好的线性关系,相关系数为0．9998,检测下限可达10^-10mol／L．该器件可用于H2O2的流动注射检测,测定线性范围为1×10^-4至5×10^-3mol／L,相关系数为0．9993,检测下限为5×10^-5mol／L．考察该器件在评估葡萄（美国进口红提）籽核和肉质的抗氧化性能方面的应用,经换算得出它们的抗氧化性能;红提籽为121mg H2O2／g,红提肉为5．1mg H2O2／g．即红提籽的抗氧化能力明显强于红提肉．在实际样品测定中,该流动注射电化学发光池具有较好的重现性,它结合了电化学发光的高灵敏度和流动注射的实用性,操作方便．     

肌乳酸跨膜运输

乳酸生成后的代谢去向主要是氧化清除.乳酸可以生成部位通过组织间隙和血液穿梭到邻近的高氧化能力部位.乳酸跨膜运输是在一元羧化乳酸运输蛋白(MCT)协助下沿浓度梯度和PH进行的.通过训练,骨胳肌和心肌细胞的MCT1含量会增加,结果摄取乳酸增加,乳酸生成后从骨胳肌外流增加.更重要的是,线粒体膜也含有MCT,这有助于乳酸在此进行氧化.     

FTIR Measurement of Cellulose Microfibril Angle in Historic Scots Pine Wood and Its Use to Detect Fungal Decay

Microfibril angle (MFA) – the orientation of cellulose fibres in the S2 layer of the secondary cell wall – is a key determinant of the stiffness and strength of timber. The microfibril angle depends on the way in which the timber was grown and its position within the tree. Microfibril angle can be measured by X-ray diffraction and other methods, but the methods in current use are slow or require advanced instrumentation. The aim of this study was to explore the use of polarised Fourier transform infrared (FTIR) microscopy as a relatively fast and inexpensive method for measuring MFA in historic Scots pine (Pinus sylvestris L.). The FTIR measurements were calibrated against X-ray measurements of MFA in modern Scots pine. We observed a wide range in MFA values and a radial pattern of MFA similar to modern Scots pine in undecayed Scots pine heartwood from sixteenth and seventeenth century beams in Scottish secular buildings. The density of the heartwood was also similar to modern plantation-grown Scots pine despite the much slower growth rate recorded in the ring widths of the historic timber. The sapwood, which had been attacked by both insect pests and fungi, showed an erratic reduction in density and a large increase in MFA compared to the modern material. The increased sapwood MFA was attributed to selective destruction of the S2 layer of the wood cell walls by fungal decay. Using MFA measurements in conjunction with density offers the possibility to estimate the mechanical properties of sound historic pine timber, to detect fungal decay more sensitively than by density alone, and to distinguish between pest and fungal attack in a way that relates directly to the remaining mechanical performance of the timber.