耐药标志物金属硫蛋白在乳腺癌中的表达

目的:探讨金属硫蛋白(MT)在乳腺癌中表达的状况.方法:采用免疫组化S-P方法.检测57例乳腺癌中MT表达的情况.结果:MT在乳腺癌组织中的总阳性率为59.65%.浸润性导管癌、单纯癌和髓样癌中MT的阳性率分别是69.05%、80.00%、10.00%,髓样癌和其他组有显著性差异(P＜0.01).MT的表达与淋巴是否转移相关性不明显.结论:检测MT为制定化疗方案提供依据.     

血管内皮生长因子在乳腺良、恶性肿瘤表达的临床意义

目的：探讨血管内皮生长因子（VEGF）在乳癌中过表达的临床意义。方法：取乳腺腺瘤47例,乳腺癌95例,利用SABC法检测VEGF阳性率。结果：VEGF在腺瘤中阳性率为25.53%（12/47）,在乳癌中阳性率为54.75%（52/95）;在浸润性癌和有淋巴结转移组阳性率明显高于非浸润性组和无淋巴结转移组（P〈0.05）。结论：VEGF过表达提示患者预后不良,检测VEGF对化疗方案选择有指导意义。     

Enzyme dynamic study on calmodulin Ca++-Mg++-ATPase system of red blood cells inhibited by α-anordrin and probimane

To clarify the possible mechanisms of α-anordin and probimane on calmodulin Ca⁺⁺-Mg⁺⁺ ATPase system, enzyme dynamic study was carried out by determining three dynamic parameters [the substrate concentration (ATP)-response curve, dose (inhibitors)-response curve and time-response curve]. Our data have shown that the inhibitory rates of α-anordrin and probimane are unrelated to substrate (APT) concentrations, but related to calmodulin concentrations. The inhibition of α-anordrin and probimane is very quick that is completed within 1 to 5 min and can maintain more than 1 hr in the same inhibitory rates. So it is possible that α-anordrin and probimane are calmodulin competitors with calmodulin-like binding whose actions can occur by affecting the reaction balance of substrate and product on target enzymes of calmodulin (Ca⁺⁺-Mg⁺⁺-ATPase).     

浅议健身运动的防癌抗癌作用及癌症患者的健身练习方法

近年来我国肿瘤患者特别是恶性肿瘤患者人数急剧上升,患者一旦知道自己的病情之后大都悲观消极,情绪低落。大部分只注重常规治疗,忽视了健身运动带来的良好效果。文章主要介绍了运动防癌的机理以及针对不同的患者采用合适的练习方法和合适的运动量,从而提高该类人群的生活质量。     

Using bio-orthogonally catalyzed lethality strategy to generate mitochondria-targeting anti-tumor metallodrugs in vitro and in vivo

Synthetic lethality was proposed nearly a century ago by geneticists and recently applied to develop precision anti-cancer therapies. To exploit the synthetic lethality concept in the design of chemical anti-cancer agents, we developed a bio-orthogonally catalyzed lethality (BCL) strategy to generate targeting anti-tumor metallodrugs both in vitro and in vivo. Metallodrug Ru-rhein was generated from two non-toxic species Ru-N₃ and rhein-alkyne via exclusive endogenous copper-catalyzed azide alkyne cycloaddition (CuAAC) reaction without the need of an external copper catalyst. The non-toxic species Ru-arene complex Ru-N₃ and rhein-alkyne were designed to perform this strategy, and the mitochondrial targeting product Ru-rhein was generated in high yield (>83%) and showed high anti-tumor efficacy in vitro. This BCL strategy achieved a remarkable tumor suppression effect on the tumor-bearing mice models. It is interesting that the combination of metal-arene complexes with rhein via CuAAC reaction could transform two non-toxic species into a targeting anti-cancer metallodrug both in vitro and in vivo, while the product Ru-rhein was non-toxic towards normal cells. This is the first example that exclusive endogenous copper was used to generate metal-based anti-cancer drugs for cancer treatment. The anti-cancer mechanism of Ru-rhein was studied and autophagy was induced by increased reactive oxygen species and mitochondrial damage. The generality of this BCL strategy was also studied and it could be extended to other metal complexes such as Os-arene and Ir-arene complexes. Compared with the traditional methods for cancer treatment, this work presented a new approach to generating targeting metallodrugs in vivo via the BCL strategy from non-toxic species in metal-based chemotherapy.     

银杏叶槲皮素对人肝癌HepG2细胞增殖与凋亡的影响

目的探讨银杏叶黄酮单体成分槲皮素体外对人肝癌细胞HepG2增殖与凋亡的影响。方法将银杏叶黄酮单体成分槲皮素作用于体外培养的人肝癌细胞HepG2,MTT法检测其对HepG2细胞增殖的影响,缺口末端核苷标记（TUNNEL）法检测其对HepG2细胞凋亡的影响。结果银杏叶黄酮单体成分槲皮素使体外培养的人肝癌细胞HepG2的增殖效率下降,使凋亡细胞数增加（P〈0.01）,两者均呈剂量依赖效应。结论银杏叶黄酮单体成分槲皮素具有与银杏叶总黄酮相似的作用,对体外培养的人HepG2细胞增殖有抑制作用,并能诱导细胞凋亡。     

银杏叶总黄酮对人肝癌HepG2细胞凋亡的影响及其机制研究

目的探讨银杏叶总黄酮对体外培养的人肝癌HepG2细胞凋亡的促进作用及其分子机理。方法采用TUNEL法检测银杏叶总黄酮对HepG2细胞凋亡的影响,提取Bcl-2基因的mRNA,以RT-PCR法研究银杏叶总黄酮对Bcl-2基因表达的影响。结果银杏叶总黄酮使人HepG2细胞凋亡指数增加（P〈0．01）,且呈剂量依赖效应;Bcl-2基因mRNA水平随银杏叶总黄酮浓度升高而降低。结论银杏叶总黄酮可促进人HepG2细胞的凋亡过程,并使Bcl-2基因mRNA水平降低,从而起到抗肿瘤的治疗作用。     

Evaluation of pelvic lymph node coverage of conventional radiotherapy fields based on bony landmarks in Chinese cervical cancer patients using CT simulation

ncer. CT simulation may be a feasible technique for planning pelvic fields optimally and individually.     

Anti-angiogenesis effect of generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide on breast cancer in vitro

Objective: To study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAMNEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells. Methods: We examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells. Results: The compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly. Conclusion: With a low toxicity, high safety, and high transfection rate, G4PAMAMNEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies.