真核生物基因表达的转录后调控

本文概要叙述了在核mRNA加工成熟过程中各种调控基因表达的方式。     

植物抗虫基因工程的研究

在当今的农业生产中,虫害问题已成为减产的重要因素之一,随着植物基因工程的兴起与发展,该问题已在很大程度上得到解决。本文介绍了利用基因工程手段而分离到的几种抗虫基因,为抗虫基因工程的发展奠定了基础,并探讨了植物抗虫基因工程中存在问题和解决方案。     

面向对象程序设计浅析

通过分析面向对象程序设计的基本思想和技术特点及他们之间的区别和联系,介绍了面向对象程序设计的优点和发展趋势。     

PCR技术在肿瘤检测及治疗中的应用

本文概述了聚合酶链反应技术在癌基因、抑癌基因、端粒酶的活性、肿瘤病毒、肿瘤免疫的研究及常见肿瘤的检测及预后等方面的应用。     

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy

INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D…     

基因沉默及其生理学意义

基因沉默包括转录水平上的基因沉默和转录后水平上的基因沉默。发生沉默的基因可以是外源性转移基因,也可以是入侵的病毒或宿主内源性基因。双链RNA(dsRNA)作为启动因素或中间体为不同生物基因沉默所共有。基因沉默是生物体在基因调控水平上的一种自我保护机制。通过基因沉默,可以限制外来核酸的入侵;同时,基因沉默还与生物的生长发育有关。     

生物芯片及应用

本概述了生物芯片的发展历史、制作方法及其广阔的应用前景，分析了基因芯片领域的最新成果。生物芯片的发展，必将对人类生活产生深远的影响。因此，生物芯片的研究受到人们的广泛关注。     

构造器内部多态方法的行为研究

概述Java中的多态、多态方法，并通过实例阐述Java中多态的实现机制及本质。进而对构造器中调用多态方法产生的错误结果进行了分析并得出结论。     

Genetic polymorphism analyses of a novel panel of 19 X-STR loci in the Chinese Uygur ethnic minority

The population genetic data and forensic parameters of 19 X-chromosome short tandem repeat (X-STR) loci in Chinese Uygur ethnic minority are presented. These loci were detected in a sample of 233 (94 males and 139 females) unrelated healthy individuals. We observed 238 alleles at the 19 X-STR loci, with the corresponding gene frequencies spanning the range from 0.0021 to 0.5644. After Bonferroni correction (P>0.0026), there were no significant deviations from Hardy-Weinberg equilibrium. The cumulative power of discrimination in females and males, and the probability of exclusion of the 19 X-STR loci were 0.999 999 999 999 999 999 998 091, 0.999 999 999 999 966, and 0.999 999 986 35, respectively. The cumulative mean exclusion chance was 0.999 999 992 849 in deficiency cases, 0.999 999 999 999 628 in normal trios, and 0.999 999 998 722 in duo cases. The high value of the forensic parameters mentioned above revealed that the novel panel of 19 loci had important values for forensic applications in the Uygur group.     

题目：遗传改造细菌基因组的策略：基因定点突变、基因失活和基因过表达

With the availability of the whole genome sequence of Escherichia coli or Corynebacterium glutamicum, strategies for directed DNA manipulation have developed rapidly. DNA manipulation plays an important role in understanding the function of genes and in constructing novel engineering bacteria according to requirement. DNA manipulation involves modifying the autologous genes and expressing the heterogenous genes. Two alternative approaches, using electroporation linear DNA or recombinant suicide plasmid, allow a wide variety of DNA manipulation. However, the over-expression of the desired gene is generally executed via plasmid-mediation. The current review summarizes the common strategies used for genetically modifying E. coli and C. glutamicum genomes, and discusses the technical problem of multi-layered DNA manipulation. Strategies for gene over-expression via integrating into genome are proposed. This review is intended to be an accessible introduction to DNA manipulation within the bacterial genome for novices and a source of the latest experimental information for experienced investigators.