改进的乙肝病毒感染动力学模型研究

基于Nowak等提出的不带效应细胞的乙肝病毒(HBV)感染模型,本文提出了由两个系统组成的数学模型,并分析了该模型的动力学行为,最后进行了数值模拟,通过数值模拟发现,该模型对慢性HBV感染的拉米夫定治疗效果提供了可能的解释,特别是可以解释为什么Nowak等人的患者的血浆中的病毒数目在停止免疫治疗后又恢复到治疗前的水平.     

Studies on the age dependent changes in serum adenosine deaminase activity and its changes in hepatitis

Serum Adenosine deaminase (ADA) activity in normal healthy control subjects increases upto 30 years, remains steady between 31–60 years of age and shows a steep increase in the age group of 61–70 years. This was compared with serum aspartate transaminase (AST) and alanine transaminase (ALT) activity which also showed a gradual increase upto 40 years of age and decreased thereafter. The activities of serum ADA, AST and ALT increased in patients with hepatitis of all age groups compared to their respective controls. The degree of increase in the activities of the above enzymes in hepatitis, decreased with age. The present study also shows that while studying serum ADA activity in hepatitis for diagnostic purposes, the value obtained in a particular age group should be compared with normal range of values for the respective age group only.     

长春市戊型肝炎的流行病学调查

通过长春市戊型肝炎流行病学调查,了解长春市戊肝病毒在动物与人群中的感染情况为戊肝防治提供宝贵的参考资料。对长春市采集的动物血清包括猪820份、牛506份、羊273份及人血清,包括农村饲养猪人群182份、农村非饲养猪人群102份,城镇人群546份,采用戊肝病毒抗原及抗体试剂盒检测血清中的病毒抗原及抗体。结果感染率分别为猪72.80%、牛11.86%、羊10.26%、农村饲养猪人群20.88%、农村非饲养猪人群15.38%、城镇人群4.58%。结果表明戊肝病毒在长春市的动物及人群中均有流行,且猪HEV感染比牛和羊及人都普遍,农村饲养猪人群和农村非饲养猪人群感染率都远高于普通人群,为戊肝是人畜共患病提供有力证据。     

乙肝歧视的法律界定

乙肝歧视是指在法律规定的条件之外,基于对乙肝病毒的错误认识而固执地给予HBVER有差别的、常常是不合理的对待.依据不同的标准可对日常生活中常见的乙肝歧视进行不同的学理分类.对乙肝歧视所采取的矫正措施和基于真正的职业要求而给予HBVER有差别的待遇一般不认为构成歧视.     

Correlation and Multiple Regression Analyses of Pituitary Growth Hormone and Hepatic Activities in Hepatitis C Infection and Interferon Response

The prevalence of hepatitis C virus (HCV) infection varies across the world, with the highest percent of infections reported in Middle East, increasingly in Egypt. The current study aimed at examining the bio-statistical correlation and multiple regression analyses of pituitary growth hormone (GH) and liver activities among HCV genotype-4 patients treated with PEG-IFN-α plus RBV therapy. Herein, the current study was conducted on 100 HCV genotype-4 infected patients and 50 healthy controls. Patients received PEG-IFN-α/RBV for 24 weeks. Host RNA was isolated from patients’ sera for HCV genotyping and viral load determination. Moreover, the enzymatic activities of the liver, AFP, GH, PT, and CBC were performed in all volunteers. The present study resulted that the activities of the hepatic enzymes among HCV genotype-4 patients correlated together significantly. While, human GH showed a significant positive regression with pre-treatment ALT concentration in responders. Furthermore, multiple regression analysis for GH showed a significant positive correlation with pre-treatment ALT in HCV genotype-4 infected patients. We concluded that there were a putative significant relation between GH and pre-treatment ALT activity in HCV infection and response to IFN-based therapy.     

医学院校大学生丙肝知识知晓率调查研究

开展吉林市医学院校大学生丙型病毒性肝炎防治知识调查,为提高医学生对丙肝的防护知识、诊断及治疗能力提供基础资料及科学依据.方法 采用整群分层抽样的方法,对吉林市2所医学院校在校大学生进行问卷调查.结果医学生丙型肝炎防护知识总体知晓率,15项问题中有3项知晓率低于50％,有8项各年级知晓率有统计学差异（P＜0.01）,7项问题不同院系学生知晓率有统计学差异（P＜0.01）,2项问题知晓率农村和城市学生有差异,但无统计学意义（P＞0.05）.结论医学院校不同院系学生对丙肝相关知识的知晓率存在差异,应针对不同年级、不同专业进行相应的丙型肝炎相关知识培训及健康教育干预.     

HCV阴性者外周血单核细胞的染色体外环状DNA中与HCV同源的DNA序列（英文）

目的:研究与丙型肝炎病毒(HCV)基因RNA序列同源的人染色体外环状DNA(eccDNA)序列.创新点:首次从HCV阴性者eccDNA中检测到HCV 5’-非编码区(5’-NCR)基因组RNA序列,验证了我们的假设: HCV同源DNA序列存在于人的外周血单核细胞的eccDNA组分中.方法:用分离的eccDNA进行HCV特异的聚合酶链反应(PCR),采用核苷酸序列同源性搜索分析软件(BLASTn)对测序结果进行比对分析,并检测其甲基化模式.结论:实验结果证实了我们的假设:即部分HCV 5’-NCR基因组RNA序列存在于外周血单核细胞的eccDNA组分.同时,甲基化分析结果显示了个体间的甲基化模式所代表的受遗传调控的表观遗传特征.     

纳米金修饰金电极固载抗体电流型乙型肝炎免疫传感器的研究

在金电极表面自组装L-半胱胺酸,然后吸附金溶胶,再将乙型肝炎表面抗体(HbsAb)固定在金纳米颗粒上,制成用于检测乙型肝炎表面抗原(HbsAg)的一种新型电流型免疫传感器。通过循环伏安法考察了电极的电化学特性。该传感器制作简单、响应时间短(2min)、选择性好和灵敏度高,该免疫传感器对乙型肝炎表面抗原(HbsAg)检测的线性范围是5μg/L～130μg/L,检出限为0．86μg/L。     

DIC、溶栓治疗、恶性肿瘤及乙型肝炎D-二聚体检测结果分析

目的探讨D-二聚体检测在DIC、溶栓治疗、恶性肿瘤及乙型肝炎等疾病中应用的临床价值.方法收集40例正常对照组和71例病例组,均采用D-二聚体试剂盒进行检测,检测原理为胶乳凝集试验.结果各检测组D-二聚体水平显著高于对照组(P＜0.01).结论D-二聚体检测对DIC、溶栓治疗、恶性肿瘤及乙型肝炎疾病的诊断、治疗及疗效观察提供一定的依据.     

乙型肝炎DNA疫苗的研究进展

乙肝DNA疫苗,它既能预防乙型肝炎,又能治疗乙型肝炎,同传统的疫苗相比具有多方面的优越性,是一种很有发展前途的疫苗．目前研究认为,乙型肝炎DNA疫苗很可能成为预防和治疗乙型肝炎的全新一代疫苗．