Correlation and Multiple Regression Analyses of Pituitary Growth Hormone and Hepatic Activities in Hepatitis C Infection and Interferon Response

The prevalence of hepatitis C virus (HCV) infection varies across the world, with the highest percent of infections reported in Middle East, increasingly in Egypt. The current study aimed at examining the bio-statistical correlation and multiple regression analyses of pituitary growth hormone (GH) and liver activities among HCV genotype-4 patients treated with PEG-IFN-α plus RBV therapy. Herein, the current study was conducted on 100 HCV genotype-4 infected patients and 50 healthy controls. Patients received PEG-IFN-α/RBV for 24 weeks. Host RNA was isolated from patients’ sera for HCV genotyping and viral load determination. Moreover, the enzymatic activities of the liver, AFP, GH, PT, and CBC were performed in all volunteers. The present study resulted that the activities of the hepatic enzymes among HCV genotype-4 patients correlated together significantly. While, human GH showed a significant positive regression with pre-treatment ALT concentration in responders. Furthermore, multiple regression analysis for GH showed a significant positive correlation with pre-treatment ALT in HCV genotype-4 infected patients. We concluded that there were a putative significant relation between GH and pre-treatment ALT activity in HCV infection and response to IFN-based therapy.     

医学院校大学生丙肝知识知晓率调查研究

开展吉林市医学院校大学生丙型病毒性肝炎防治知识调查,为提高医学生对丙肝的防护知识、诊断及治疗能力提供基础资料及科学依据.方法 采用整群分层抽样的方法,对吉林市2所医学院校在校大学生进行问卷调查.结果医学生丙型肝炎防护知识总体知晓率,15项问题中有3项知晓率低于50％,有8项各年级知晓率有统计学差异（P＜0.01）,7项问题不同院系学生知晓率有统计学差异（P＜0.01）,2项问题知晓率农村和城市学生有差异,但无统计学意义（P＞0.05）.结论医学院校不同院系学生对丙肝相关知识的知晓率存在差异,应针对不同年级、不同专业进行相应的丙型肝炎相关知识培训及健康教育干预.     

柴芍六君子汤加味治疗慢性乙型病毒性肝炎的临床观察

目的:观察柴芍六君子汤加味治疗慢性乙型病毒性肝炎(肝郁脾虚型)的临床疗效。方法:将120例符合纳入标准的患者随机分为治疗组60例和对照组60例,对照组予恩替卡韦分散片治疗,治疗组在对照组基础上给予柴芍六君子汤加味治疗,治疗12周为1个疗程。结果:1个疗程结束后,治疗组总有效率为83.33%,对照组总有效率为60%,两组比较,治疗组优于对照组(P0.05)。结论:柴芍六君子汤加味联合恩替卡韦分散片治疗CHB疗效确切,值得推广。     

乙肝专题文献知识库的创新构建

信息整合与数据挖掘技术在文献数据库创新性建设领域具有非常重要的意义,这已在国外同行的应用中取得了较好的效果,但国内对此项技术的研究和应用才刚刚起步。该研究通过广泛调研,制定了文献专题知识数据库的期刊和文献收录标准,以及数据库框架结构和数据录入标准。在此基础上,收集了国内外乙肝相关文献,借助期刊和文献影响力及专家内容分析等筛选方法筛选了近1.5万篇左右文献信息,构建了一个知识覆盖面广、文章质量高和分类标引好的乙肝专题文献知识库,并进一步通过构建文本挖掘工具字典,成功实现了乙肝专题文献知识库与国内外科学数据型数据库的关联与整合,从而初步实现了乙肝病毒相关蛋白质的知识挖掘。     

双环醇治疗慢性乙型病毒性肝炎肝纤维化及其作用机制

[目的]观察双环醇对慢性乙型病毒性肝炎患者肝纤维化程度以及肝组织内转化生长因子β1(TGF-β1)和Ⅰ、Ⅲ型胶原含量的影响.[方法]20例慢性乙型病毒性肝炎患者以双环醇75~150 mg/d治疗24周,治疗前后分别肝活检进行常规病理学检查,并应用免疫组化技术和多媒体彩色病理图文分析系统观察双环醇治疗前后肝组织内TGF-β1和Ⅰ、Ⅲ型胶原含量的变化.[结果]慢性乙型病毒性肝炎肝纤维化患者经双环醇治疗后,肝组织炎症和纤维化程度明显改善,肝组织内TGF-β1和Ⅰ、Ⅲ型胶原含量均较治疗前明显降低,差异有显著性(P<0.05).[结论]双环醇治疗可以减轻慢性乙型病毒性肝炎患者肝脏炎症反应,同时明显降低慢性乙型病毒性肝炎患者肝组织内TGF-β1和Ⅰ、Ⅲ型胶原的含量,从而发挥抗纤维化作用.     

大学生接种乙型肝炎疫苗不良反应的观察及干预措施——以盐城师范学院为例

目的对大学生乙型肝炎疫苗接种后的反应进行观察、分析,以更好的降低接种不良反应的发生。方法 2010年10月12日-2014年4月30日中,来本所加强免疫接种乙肝疫苗共907人,有213例出现不同程度的不良反应,对该结果进行统计分析。结果乙型肝炎疫苗接种后主要为正常免疫反应,不良反应发生率很低。结论预防接种乙型肝炎疫苗(HBV)是控制乙肝发生最科学、经济和有效的手段,对其不良反应有充分的认识,掌握应对措施,加强宣传和预防。     

Green tea polyphenol,epigallocatechin-3-gallate,possesses the antiviral activity necessary to fight against the hepatitis B virus replication in vitro

Although several antiviral drugs and vaccines are available for use against hepatitis B virus (HBV), hepatitis caused by HBV remains a major public health problem worldwide, which has not yet been resolved, and new anti-HBV drugs are in great demand. The present study was performed to investigate the anti-HBV activity of epigallocatechin-3-gallate (EGCG), a natural-origin compound, in HepG2 2.2.15 cells. The antiviral activity of EGCG was examined by detecting the levels of HBsAg and HBeAg in the supernatant and extracellular HBV DNA. EGCG effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose- and time-dependent manner, and it showed stronger effects at the level of 0.11–0.44 μmol/ml (50–200 μg/ml) than lamivudine (3TC) at 0.87 μmol/ml (200 μg/ml). EGCG also suppressed the amount of extracellular HBV DNA. The data indicated that EGCG possessed anti-HBV activity and suggested the potential of EGCG as an effective anti-HBV agent with low toxicity.     

拳卷地钱总黄酮提取物抗乙型肝炎病毒体外实验研究

目的:通过体外实验,探讨拳卷地钱总黄酮提取物抗乙肝病毒的作用。方法:将拳卷地钱总黄酮提取物作用于HBV-DNA转染细胞系2.2.15细胞,通过检测细胞培养液中HBsAg、HBeAg的变化来评价拳卷地钱总黄酮提取物抗乙肝病毒的效果及其可能的药理活性部位。结果:当拳卷地钱总黄酮提取物的最大无毒浓度为31.3μg·mL-1,对2.2.15细胞HBeAg、HBsAg分泌抑制率分别为51.56%、39.69%;治疗指数(TI)>2。结论:在2.2.15细胞无毒浓度下,拳卷地钱总黄酮对2.2.15细胞HBsAg、HBeAg分泌具有比较好的抑制作用;且可以有效阻止HBV-DNA表达和复制。提示:拳卷地钱总黄酮提取物在体外有一定的抗乙肝病毒作用。     

3种甘草酸苷对慢性乙肝患者血糖及肝功能病毒影响的临床研究

[目的]了解甘草酸苷对慢性乙肝患者血糖的影响.[方法]把95例确诊为慢性乙型肝炎的患者随机分为A、B、C三组,分别给予复方甘草酸苷80 m l、复方甘草酸单铵120 m l、甘草酸二铵40 m l静脉点滴,用药前、用药2周及4周观察三组患者的空腹血糖、肝功能、HBVM、HBV-DNA定量.[结果]治疗前三组患者空腹血糖的均数分别为5.28±1.75、5.32±1.66及5.65±1.82 mmol/L,在用药2周时,三组患者空腹血糖的均数即已有所下降,至用药4周其空腹血糖的均数分别为4.5 1±1.6 5、4.6 8±1.3 7及4.9 8±1.87 mmol/L,与治疗前相比三组患者空腹血糖的均数都有不同程度下降,但治疗前后的差异无显著性(P>0.05),各组之间下降的差异亦无显著性(P>0.05).[结论]甘草酸苷对慢性乙肝患者的血糖没有升高作用,反而在一定剂量下有降低的作用.