In vitro study on ribozyme against hepatitis C virus

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.     

CeO2载体对Au/CeO2催化剂CO氧化活性的影响

采用六水硝酸铈和氢氧化钠为反应物,用水熟合成法制备了纳米Ce02.并以不同合成条件下得到的CeO2为栽体,担载了相同含量的贵金属Au,制备了具有高活性的Au/CeO2催化荆.实验结果发现,以CeO1-2h为载体的催化剂在高温区易展现出较好的活性,而以CeO2-10h为载体的催化剂在低温区易展现出较好的活性.说明载体结构对催化荆的CO催化氧化活性有决定性的作用.     

基于MVC的主流Web框架技术研究

随着Web应用的普及和用户需求的多样化、复杂化,基于J2EE和.Net平台的Web框架也逐渐暴露出诸多缺陷。Rails则是一种基于MVC模式、建立在Ruby语言基础上的新一代Web应用程序框架,在简化开发过程、降低开发成本、缩短开发周期和部署Web应用等方面,克服了传统的Web框架技术的不足,具有良好的应用前景。     

运动员竞赛焦虑状态的分析

以参加第六届全国农运会田径项目的150名运动员作为研究对象,采用＂运动竞赛状态焦虑量表＂（简称CSAI-2问卷）为测试工具进行调查.结果表明：（1）不同性别的运动员竞赛焦虑状态各维度上不存在显著差异,但女运动员竞赛焦虑状态各因素得分略高于男运动员;（2）不同运动年限的运动员竞赛焦虑状态各维度上存在显著差异,运动年限越长竞赛焦虑状态各因素得分越高;（3）不同专项的运动员竞赛焦虑状态各维度不存在显著差异.     

话语标记语“I think”的韵律分析

话语标记语是动态话语中十分常见的一种语言现象。本文从韵律的角度,结合声音的波形文件直观地探讨了话语标记语＂I think＂的语用功能,以期为话语标记语方面的研究补充一种新的视角。     

新建地方本科院校课堂教学评价体系新论——基于邯郸学院“234”课堂教学评价体系个案研究

课堂教学评价体系的建构与实施对提高教学质量起着重要的引领作用。对照目前我国高校课堂教学评价体系现存的主要问题,新建地方本科院校应该以先进的教育理论和现代质量管理理论为参照,在外适型质量观的指导下,构建富有现实针对性的评价体系。邯郸学院234课堂教学评价体系在这方面进行了初步探索和研究。     

基于Proteus和Keil的数字电子钟的设计与仿真

Proteus和Keil作为单片机系统开发的主流工具,具有节约硬件成本、缩短研发周期、提高设计成功率的特点.以AT89C52、DS1302和LCD1602为主要元器件,设计和仿真一个数字电子钟系统.     

基于网络协作模式的教学资源建设与实践——以C程序设计课程为例

基于网络协作学习理论,围绕＂C程序设计＂课程建设目标,在建设立体化课程教学资源的同时,构建了基于Wiki的网络协作教学支撑平台。通过教学实践,不仅强化了优质教学资源的共建共享,也对进一步提高课程教学质量,促进人才培养模式改革产生了积极作用。     

维生素C和免疫多糖对中华倒刺鲃非特异性免疫功能的影响

在中华倒刺鲃每千克基础饲料中分别添加免疫多糖2g、免疫多糖4g、维生素C（VC）700mg、VC1400nag、VC700mg＋免疫多糖2g、VC700mg＋免疫多糖4g、VC1400mg＋免疫多糖2g、VC1400mg＋免疫多糖4g,以基础饲料为对照组,连续投喂2周后,通过测定血液白细胞的数量,白细胞吞噬能力,血清、头肾和脾脏的溶菌酶活力,探讨不同浓度的免疫多糖和VC对中华倒刺鲃非特异性免疫功能的影响．结果表明：VC和免疫多糖都能提高中华倒刺鲃免疫机能,且免疫多糖和VC二者间在较低浓度组合时有协同作用,高浓度组合时反而产生拮抗作用．免疫多糖和VC的最适宜组合浓度为（VC700mg＋免疫多糖2g）／kg基础饲料．     

Improving Writing Skills of Thai EFL Students by Recognition of and Compensation for Factors of L 1 to L2 Negative Transfer

In this study, we examined the likelihood of a TAME （extended technology acceptance model）, in which the interrelationships among computer self-efficacy, perceived usefulness, intention to use and self-reported use of computer-mediated technology were tested. In addition, the gender- and age-invariant of its causal structure were evaluated. The data were collected from a self-reported questionnaire administered to 477 administrative staff of a public university in Malaysia. The results of structural equation modeling supported the adequacy of TAME. Although the TAME＇s causal structure was applicable to both male and female staff, age group appeared to moderate the structural relationships among the constructs of interest.