Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro

BackgroundCecropin P1, acting as an antimicrobial, has a broad-spectrum antibacterial activity with some antiviral and antifungal properties. It is a promising natural alternative to antibiotics which is originally isolated from the pig intestinal parasitic nematode Ascaris suum. Many studies have shown that Cecropin P1 is helpful for the prevention or treatment of clinical diseases. Therefore, it is very necessary to establish a safe, nontoxic, and efficient expression method of Cecropin P1.ResultsThe results indicated that the recombinant protein was about 5.5 kDa showed by Tricine–SDS–PAGE and Western blot. And Cecropin P1 was efficiently secreted and expressed after 12 h of induction, with an increasing yield over the course of the induction. Its maximum concentration was 7.83 mg/L after concentration and purification. In addition, in vitro experiments demonstrated that Cecropin P1 not only exerted a strong inhibitory effect on Escherichia coli, Salmonella sp., Shigella sp., and Pasteurella sp., but also displayed an antiviral activity against PRRSV NADC30-Like strain.ConclusionsCollectively, the strategy of expressing Cecropin P1 in Saccharomyces cerevisiae is harmless, efficient, and safe for cells. In addition, the expressed Cecropin P1 has antiviral and antibacterial properties concurrently.How to cite: Jiang R, Zhang P, Wu X, et al., Expression of antimicrobial peptide Cecropin P1 in Saccharomyces cerevisiae and its antibacterial and antiviral activity in vitro. Electron J Biotechnol 2021;50. https://doi.org/10.1016/j.ejbt.2020.12.006     

中国水生植物新记录属——钻叶荠属(十字花科)

 报道中国十字花科一新记录属¾钻叶荠属Subularia L. 及一新记录种¾钻叶荠S. aquatica L.。     

中国西部柳属新植物

 Two new species and one new variety of Salix L. from western China, namcl. S. ludingensis T. Y. Ding et C. F. Fang, S. neoamnematchinensis T. Y. Ding et C. F. Fang, and S. taoensis Gorz var. leiocarpa T. Y. Ding et C. F.Fang, are reported in this paper.     

“大学域” 图书馆资源共享研究

随着高等教育规模的扩大，大学城在我国得到了迅速的发展。文章分析了大学城在我国产生的必然性，大学城资源共享的主要领域，提出了大学城图书馆之间可以在政策制定、协调采购、目录编制、馆际互借、对外交流和利用现代信息技术方面实现资源共享，分析了在资源共享方面存在的问题。     

Knowledge-relatedness in firm technological diversification

This paper claims that knowledge-relatedness is a key factor in affecting firms’ technological diversification. The hypothesis is tested that firms extend the range of their innovative activities in a non-random way. Specifically, we test the extent to which firms diversify their innovative activities across related technological fields, i.e. fields that share a common knowledge base and rely upon common heuristics and scientific principles. The paper proposes an original measure of knowledge-relatedness, using co-classification codes contained in patent documents, and examines the patterns of technological diversification of the whole population of firms from the United States, Italy, France, UK, Germany, and Japan patenting to the European Patent Office from 1982 to 1993. Robust evidence is found that knowledge-relatedness is a major feature of firms’ innovative activities.     

基于5W1H分析的公共图书馆大字本图书阅读推广服务探究

大字本图书是低视力人群重要的信息来源和学习资源,公共图书馆作为大字本图书主要的馆藏地,肩负着大字本图书阅读推广服务的重要职责。通过5W1H分析法,提出公共图书馆大字本图书阅读推广服务对策,包括服务时间、地点的选择、服务团队的建设以及服务方法等,以期对公共图书馆开展大字本图书阅读推广服务有所帮助。     

高职高等数学"2+1"教学模式研究与实践

针对目前高职数学课程教学内容体系一成不变,教学方式单一,教学模式陈旧的现状,高职院校要转变教学思想,改革课程体系,以应用能力培养为主线,通过构建“2＋1”（二年理论教学＋一年实践教学）模式来提高教学效率,并通过改革教学方法和教学手段以保证“2＋1”教学模式的顺利实施。     

Habilitationsverfahren; Gutachten; Beweiswürdigung

Der Umstand, dass fünf Gutachten zum Ergebnis gelangt seien, die im Habilitationsverfahren vorgelegten Arbeiten würden die wissenschaftliche Qualifikation des Habilitanden iSd § 28 Abs 5 UOG 1993 erweisen, w?hrend nur eines eine gegenteilige Auffassung vertrete, besagt für sich nichts über die inhaltliche Richtigkeit der einen oder der anderen Auffassung. Eine Bindung der Beh?rde an die "klare Mehrheit" der in den eingeholten Gutachten vertretenen Auffassung besteht nicht. Vielmehr ist iSd § 45 Abs 2 des im Habilitationsverfahren anzuwendenden AVG der im Wege der Beweiswürdigung zu ermittelnde "innere Wahrheitsgehalt" der Ergebnisse des Beweisverfahrens ma?geblich, wobei die bei der Beweiswürdigung ma?gebenden Erw?gungen in der Begründung des Bescheides klar und übersichtlich zusammenzufassen sind.     

母语思维对儿童第二语言习得的积极影响

关于母语思维与二语习得的关系,学界已有不少论述,但大多注重研究母语思维对二语习得的消极负面影响,对其积极正面影响则探讨不多。造成这一问题的关键就是认识研究的方法是机械的、片面的,而非辩证的、科学的。母语思维与二语习得具有三重关联性,即外在关联性、本质关联性和过程关联性,因此母语思维在儿童二语习得过程中,除了其消极影响外,它还有助于儿童理解、消化和吸收第二语言信息,进而帮助儿童更好地完成第二语言习得。