Antioxidant activity ofcurculigo orchioides in carbon tetrachloride—induced hepatopathy in rats

In this study antioxidant activity of methanol extract of rhizomes ofCurculigo orchioides (MEC) was investigated using carbon tetrachloride (CCl₄)-intoxicated rat liver as the experimental model. The hepatotoxic rats were administered MEC for 90 days (daily, orally at the dose of 70 mg per kg body weight). Lipid peroxidation (LPO) in CCl₄-intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances (TBARS) and diene conjugates (CD), and also a distinct diminution in glutathione (GSH) content in the liver. In CCl₄+MEC—treated rats these biochemical parameters attained an almost normal level. The decreased activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GRD) in CCl₄—intoxicated rats, and its retrieval towards near normalcy in CCl₄+MEC—administered rats revealed the efficacy of MEC in combating oxidative stress due to hepatic damage. Elevated level of glutathione transferase(GTS) observed in hepatotoxic rats too showed signs of retuming towards normalcy in MEC co-administered animals, thus corroborating the antioxidant efficacy of MEC. The findings provide a rationale for further studies on isolation of active principles and its pharmacological evaluation.     

Review:Lipids changes in liver cancer

Liver is one of the most important organs in energy metabolism. Most plasma apolipoproteins and endogenous lipids and lipoproteins are synthesized in the liver. It depends on the integrity of liver cellular function, which ensures homeostasis of lipid and lipoprotein metabolism. When liver cancer occurs, these processes are impaired and the plasma lipid and lipoprotein patterns may be changed. Liver cancer is the fifth common malignant tumor worldwide, and is closely related to the infections of hepatitis B virus (HBV) and hepatitis C virus (HCV). HBV and HCV infections are quite common in China and other Southeast Asian countries. In addition, liver cancer is often followed by a procession of chronic hepatitis or cirrhosis, so that hepatic function is damaged obviously on these bases, which may significantly influence lipid and lipoprotein metabolism in vivo. In this review we summarize the clinical significance of lipid and lipoprotein metabolism under liver cancer.     

日粮中添加铜对小鼠血清和肝脏中铜、锌、锰的影响

96只小白鼠,随机分为4组.日粮中铜的添加量分别为0、60、120、180mg/kg,进行为期30d的试验,观察饲料中添加铜对小鼠血清和肝脏中铜、锌、锰的影响.结果表明:小鼠肝脏、血清中铜水平随日粮中铜添加量的升高而明显升高(P＜0.05),而肝脏、血清中锌、锰含量明显下降(P＜0.05).铜与锌、锰吸收与代谢之间存在颉抗关系.     

肝灌注异常的CT表现及原因分析

目的探讨肝灌注异常的CT表现及产生原因,提高对其影像学表现认识。方法从3248例肝脏平扫＋增强扫描病例中．选取符合肝脏灌注异常产生条件且有完整临床资料、相关辅助检查证实的578例,进行回顾性分析总结。结果肝脏灌注异常发生于动脉期,分高密度和低密度灌注;多呈三角形、楔形、新月形及椭圆形;高密度灌注465例,低密度灌注113例;产生原因为肝原发和继发性肿瘤、肝硬化、感染性病变及肝外伤和肝医源性损伤。结论肝脏灌注异常在增强扫描中,有特定的产生时期、部位和影像学表现,原因多种多样;熟悉该特点有助于提高对肝脏疾病的认识,减少误诊。     

人肝癌细胞总RNA电转染树突状细胞对T细胞的激活效应

目的:采用Trizol一步法提取人肝癌细胞总RNA电转染人外周血树突状细胞(Dendritic Cell,DC),观察其对混合T淋巴细胞的体外激活效应。方法:通过密度梯度离心法分离人外周血单核细胞,用细胞因子体外培养诱导其成为DC。Trizol一步法提取人肝癌细胞总RNA。通过电转染法将人肝癌细胞总RNA导入DC内;通过混合淋巴细胞实验,获取DC激活的特异性效应T细胞。用MTT法测定效应T细胞的增殖率。结果:电转染方法可将人肝癌细胞总RNA导入DC;电转染前后DC分子表达无显著差异。转染了人肝癌细胞总RNA的DC可特异的激活T细胞且增殖率明显增强(P<0.05)。结论:电转染为人肝癌细胞总RNA导入DC提供技术上的可行性;转染了人肝癌细胞总RNA的DC可特异激活T细胞。     

肝衰竭近代中医症候的研究概述

综述近年来肝衰竭的中医病因病机、证候研究进展,探讨了慢性肝衰竭的治疗方法,为该病的临床诊治提供了新思路和新方法。     

鸡传染性贫血病弱毒苗免疫对肝线粒体抗氧化酶活性的影响

观察鸡传染性贫血病弱毒苗多次免疫对肝线粒体中抗氧化酶活性的影响。方法：20只SPF鸡随机分为2组,每组10只,免疫组鸡用鸡传染性贫血弱毒苗免疫四次,每次间隔2W,对照组鸡用同剂量的生理盐水。最后一次免疫后10d取肝脏制备线粒体,利用测试盒测定肝线粒体中的GSH-PX活性、SOD活性和MDA含量。结果：对照组肝线粒体中GSH-Px活性、SOD活性和MDA含量分别为20．444±2．64U／mgprot、15．59±1．33U／mgprot和7．32＋1．89nmol／mL,免疫组肝线粒体中GSH-Px活性、SOD活性和MDA含量分别为24．85±3．53U／mgprot、24．284±4．17U／mgprot和5．264±1．24nmol／mL。与对照组相比,免疫组肝线粒体中GSH-PX活性显著提高（P〈0．05）,SOD活性极显著提高（P〈0．01）,MDA含量显著减低（P〈0．05）。结论：鸡传染性贫血病多次免疫可提高机体抗氧化能力。     

耐力训练及限食对老龄大鼠骨骼肌线粒体氧化及抗氧化水平影响的比较研究

目的:观察长期耐力训练及限食对老龄大鼠骨骼肌线粒体氧化及抗氧化水平的影响,比较其单独及协同作用,为"线粒体衰老"学说提供一定的理论依据。方法:32只17月龄雄性SD大鼠分为4组:安静组(Control,C)、限食组(Caloric-Restricted,CR)、运动组(Exercise,E)和限食加运动组(Caloric-restricted and Exercise,E+CR),训练方式为跑台运动,中等运动强度(64%VO2max,15m/min,60分钟/天,每周5天),限食摄入的标准为正常摄入组的60%,共训练及限食12周,相同月龄对照组正常饲养。12周后于末次训练后取大鼠骨骼肌分别进行线粒体氧化损伤水平及抗氧化水平测定。结果:CR组、E组和CR+E组骨骼肌线粒体丙二醛(malondialdehyde,MDA)含量显著降低,锰超氧化物歧化酶(manganese-containing superoxide dismutase,MnSOD)活性显著增加,谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-PX)活性显著增加;E组和CR+E组骨骼肌线粒体过氧化氢酶(catalase,CAT)活性显著增加。结论:耐力训练提高机体的抗氧化水平,同时使细胞机能节省化,耗氧量相对下降而减少了自由基的产生,限食减弱了机体的氧化应激,减少了自由基的产生,阻止了脂质过氧化,同时限食动员了一系列的适应性的网络防御机制,增加了抗氧化水平,加强了防御。耐力运动在机体抗氧化能力上明显强于限食作用,而耐力运动与限食的协同作用对某些抗氧化酶的影响强于其单独作用的影响。     

Ciliated hepatic foregut cyst: report of first case in China and review of literature

Objective: To report the first case of ciliated hepatic foregut cyst in China, and review of literature to introduce the characteristics of this disease for doctors to recognize this disease. Method: Report the clinical procedure of diagnosis and treatment for the first case of ciliated hepatic foregut cyst in China, and to review the embryologic genesis, incidence, clinical manifestation, radiologic features and therapeutic principle of this disease. Results: We performed the resection for ciliated hepatic foregut cyst under laparoscopy; the patient recovered well after the procedure. Conclusion: Ciliated hepatic foregut cyst is quite rare clinically, belongs to non-parasitic, solitary and unilocular cystic lesion, is always less than 4 cm in diameter, mostly seen in the left lobe, and has the tendency of malignant change. It should be removed as soon as diagnosed.     

肝脏局灶性结节增生的影像学诊断价值

目的探讨肝脏局灶性结节增生(focal nodular hyperplasia,FNH)的影像学诊断价值.方法回顾性分析经病理证实的肝脏局灶性结节增生18例22个病灶的US、CT和MR影像资料.结果US、CT和MR非造影检出率:US13个,占59.1%(13/22);CT检出16个,占72.7%(16/22);MR显示全部病灶,占100%(22/22).US、CT和MR造影检出率:US共检出17个,占77.3%(17/22);CT共检出19个,占86.4%(19/22);MR全部检出,占100%(22/22).正确诊断:US27.3%(6/22),CT40.9%(9/22),MR68.2%(15/22).影像综合正确诊断:90.9%(20/22).结论影像学综合检查能够正确诊断大部分肝脏局灶性结节增生,MRI诊断准确性明显优于CT和US.