Evaluation of microsatellite instability in tumors of central nervous system: A pilot study

Microsatellite instability (MSI) characterized by alterations at simple repetitive genomic sequences is a distinct mechanism in tumorogenesis. Central nervous system (CNS) tumors have been reported to exhibit MSI, indicator of defective mismatch repair system with controversies. The present study was undertaken to examine sixteen primary brain and two spinal tumors for MSI at six mono: BAT-26, BAT-40, BAX, TGFßRII, IGFIIR and hMSH3 and four dinucleotide loci: D2S123, D9S1851, D9S283 and D18S58. Polymerase chain reaction (PCR) was done to amplify tumour and blood DNA, analyzed on 8% denaturing Polyacrylamide gel followed by autoradiography. Out of 18 CNS tumors examined, 39% exhibited MSI at BAT-26, BAT-40, D9S1851, D9S283 and D18S58 in tumoral DNA. However, no alteration was observed at BAX, TGFßRII, IGFIIR, hMSH3 and D2S123 loci. Low incidence of MS1-high hypothesizes role of MSI in evolution of CNS tumors but not in cancer initiation or progression.     

话语标记的功能

Discourse markers are pervasive in conversation, generally used to facilitate human communication.With respect to different contextual effects that discourse markers have in utterance interpretation, they may function subjectively, interactionally or textually.     

基于分子印迹聚合物的水杨酸电导型传感器

以水杨酸为模板分子,通过制备印迹聚合物作为敏感材料,制成检测水杨酸的电导型传感器。测试了水杨酸印迹聚物[P(SA)]对水杨酸(SA)的吸附特性。该传感器在pH6.0的缓冲介质中,对浓度范围为0.01-0.1mmol L-1的水杨酸有明显的电导响应,寿命为5个月。通过对传感器选择性,灵敏度和稳定性测试,结果证明水杨酸分子印迹聚合物可以作为敏感物质用于水杨酸传感器的开发研制。     

“不是我说你”类话语标记语试说

现代汉语中话语标记语在语法上是独立的,不和句子的其他成分发生结构联系,但有其自身实在语义。在考察＂不是我说你＂类语言成分表现形式的基础上,本文给出判定标准,界定＂不是我说你＂类语言成分是现代汉语中一类特殊的话语标记语,最后本文分析了其结构形式和语法特征。     

语用标记语与贸易谈判策略

语用标记语能帮助说话人有效地传达信息,也可帮助听话者更好理解说话者的意图,因此在贸易谈判中,谈判双方可以充分利用语用标记语的功能,委婉地表明交际意图,最大限度达成谈判一致,建立双方长久的贸易关系。     

分子伴侣的作用探析

分子伴侣不仅是一大类在蛋白质折叠、组装、复性、转运和降解等方面具有重要功能的蛋白质,随着研究的深入,还发现分子伴侣而且是在病毒感染、DNA复制、转录、抗衰老和信号转导等方面也具有重要的功能,本文对此进行了综述。     

自然口语中的话语标记“得了吧”

话语标记是自然口语交际中一种十分普遍的话语现象,它对交际的成功进行起重要作用。文章对"得了吧"的话语标记性质进行了确认,归纳了其使用模式,分析了其语用功能。     

Studying new forms of participation and identity in mathematics classrooms with integrated communication and representational infrastructures

Wireless networks are fast becoming ubiquitous in all aspects of society and the world economy. We describe a method for studying the impacts of combining such technology with dynamic, representationally-rich mathematics software, particularly on participation, expression and projection of identity from a local to a public, shared workspace. We describe the types of mathematical activities that can utilize such unique combinations of technologies. We outline specific discourse analytic methods for measuring participation and methodologies for incorporating measures of identity and participation into impact studies.

基于建构主义的分子生物学教学探索

基于认知心理学理论,结合建构主义的优势,对传统的分子生物学的教学模式进行改革和探索。由于分子生物学课程体系庞大,知识点繁多且抽象,易使学生对其产生厌倦感,影响学习兴趣。我们通过在教学改革实践中依据课程内容特点,随机选择试点班级进行构建主义教学模式探索,教师采用引导学生进入课程主体,培养学生的主动参与自我构建,增强学生的综合素质的教学模式,使得实验班与对照班相比,在分子生物学的期末考试成绩上有显著提高(P<0.05)。因此,科学、合理的分子生物学教学体系,对培养适应现代生命科学技术发展需求、适应市场发展需要的生物技术创新人才具有重要意义,值得应用和大力推广。     

思维导图在高校生物类分子生物学教学中的应用

思维导图作为一种思维工具和辅助学习工具,通过直观化、发散化和非线性方式来帮助掌握学习内容。在分子生物学教学中使用思维导图,可以帮助学生掌握学习重点,提高学习效率,更有效地训练学生的高级思维技能。