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31.
通过离子交换、凝胶过滤、亲和层析及反向高效液相色谱操作,分析鉴定了棒纹牛蛭体内主要抗凝血物质成分为凝血酶抑制蛋白,其组成是三个相对分子质量分别为。7528,7446和7016的小分子量蛋白质,综合分析后认为属水蛭素类物质. 相似文献
32.
Shweta Saxena 《Resonance》2005,10(8):91-96
Lung surfactant, a lipo-protein complex, is a highly surface-active material found in the fluid lining the air-liquid interface
of the alveolar surface. Surfactant plays a dual function of preventing alveolar collapse during breathing cycle and protection
of the lungs from injuries and infections caused by foreign bodies and pathogens. Varying degrees of structure-function abnormalities
of surfactant have been associated with obstructive lung diseases, respiratory infections, respiratory distress syndromes,
interstitial lung diseases, pulmonary alveolar proteinosis, cardiopulmonary bypass surgery and smoking. For some pulmonary
conditions, especially respiratory distress syndrome, surfactant therapy is on the horizon. 相似文献
33.
Mcm7是微小染色体维持蛋白家族成员之一,与其他的Mcm蛋白形成异多聚体,调节DNA的复制。它与转录成分关联,可能与RNA合成即转录有关,并且增殖细胞或组织高表达Mcm7,可以作为肿瘤生物标志的候选因子。 相似文献
34.
INTRODUCTION Bone morphogenetic proteins (BMPs) are agroup of proteins that can induce new bone forma-tion at orthotopical and heterotopical sites in ex-perimental animals (Urist et al., 1983; Groeneveldand Burger, 2000). Their biological activity hasstimulated interest in its clinical use for bone repair.In clinical use, BMPs are undergoing evaluationincluding system administration, gene transfer andlocal matrix delivery vehicle implantation (Kirker-Head, 2000; King et al., 199… 相似文献
35.
36.
脂肪酸的从头合成主要发生在肝脏和脂肪组织,肝脏脂肪酸的合成受到多种因素的调节,文章重点介绍调节脂肪酸合成的核转录因子:肝脏X受体固醇调节元件结合蛋白、碳水化合物反应元件结合蛋白。 相似文献
37.
Li-rong Chen Hong-wei Zhou Jia-chang Cai Rong Zhang Gong-xiang Chen 《Journal of Zhejiang University. Science. B》2009,10(5):348-354
Objective To investigate the mechanism of carbapenem resistance and the occurrence of plasmid-mediated quinolone resistance determinants
qnr and aac(6′)-Ib-cr in a clinical isolate of Enterobacter cloacae.
Methods An ertapenem-resistant E. cloacae ZY106, which was isolated from liquor puris of a female gastric cancer patient in a Chinese hospital, was investigated. Antibiotic
susceptibilities were determined by agar dilution method. Conjugation experiments, isoelectric focusing, polymerase chain
reaction (PCR), and DNA sequence analyses of plasmid-mediated carbapenemases and quinolone resistance determinants were preformed
to confirm the genotype. Outer membrane proteins (OMPs) were examined by urea-sodium dodecyl sulfatepolyacrylamide gel electrophoresis
(Urea-SDS-PAGE).
Results Minimum inhibitory concentrations (MICs) of imipenem, meropenem, and ertapenem for ZY106 were 2, 4, and 16 μg/ml, respectively.
Conjugation studies with Escherichia coli resulted in the transfer of significantly reduced carbapenem susceptibility. ZY106 produced IMP-1 metallo-β-lactamase and
CTX-M-3 extended-spectrum β-lactamase, and E. coli transconjugant produced IMP-1. Plasmid-mediated quinolone resistance determinant qnrS1 was detected in ZY106. Transfer of the qnrS1-encoding-plasmid into E. coli by conjugation resulted in intermediate resistance to ciprofloxacin in E. coli transconjugant. Urea-SDS-PAGE analysis of OMPs showed that ZY106 lacked an OMP of approximately 38 kDa.
Conclusion It is the first IMP-1-producing Enterobacteriaceae in China and the first report of a clinical isolate that harbors both blaIMP and qnrS genes as well. The blaIMP-1, blaCTX-M-3, and qnrS1 are encoded at three different plasmids. IMP-1 combined with the loss of an OMP possibly resulted in ertapenem resistance
and reduced imipenem and meropenem susceptibility in E. cloacae.
相似文献
38.
Glycated hemoglobin levels in hemolysate of normal and diabetic patients were determined by the 2,6-dimethylphenol:57.5% sulphuric
acid conventional method and the values were 0.39±025 and 0.69±0.21 moles of hydroxymethylfurfural(HMF)/mole of globin, respectively.
The mean increase in glycated hemoglobin values in diabetics (1.8fold) was highly significant (p<0.001). A good correlation
(r=0.95) was found between the glycated hemoglobin values obtained by this method and the phenol:sulphuric acid method. The
values obtained by former method were about 1.2–1.4 times the values by the phenol:sulphuric acid method. This study indicates
that conventional 2,6-dimethylphenol: 57.5% sulphuric acid method is more sensitive for the estimation of glycated hemoglobin
than any other method based on the same principle. It is less time consuming, reliable and hence can be employed for the routine
laboratory estimation of glycated hemoglobin for the assessment of glycemic control. 相似文献
39.
小麦胚生物活性肽产品开发研究进展 总被引:1,自引:0,他引:1
小麦胚芽中不但富含维生素E、维生素B等多种B族维生素,还含有膳食纤维、矿物质和活性多肽等生物活性物质,已成为当前食品工业中新产品开发的重要方面.本文阐述了小麦胚生物活性肽的营养价值和小麦胚多肽产品的开发研究现状.旨在为进一步开发小麦胚多肽产品提供参考. 相似文献
40.
Membrane lipid and protein composition was compared in erythrocytes from iron deficiency anemia (IDA) and heterozygous beta
thalassemia patients. The study was planned to correlate the influence of iron deficiency with the intrinsic defect of the
heterozygous condition on the membrane structural integrity as well as to investigate whether there are differences in membrane
changes between the two conditions. Results indicate high levels of saturated fatty acids and low unsaturated fatty acids
in both disorders although arachidonic acid and the unsaturation index were lower in heterozygous thalassemia than IDA. Nevertheless,
neither of the conditions provoked any alterations in membrane protein or glycophorin suggesting alterations in the lipid
moiety only. Present findings indicate that irrespective to the etiology, both, iron deficiency and the heterozygous condition
show a common pattern of lipid derangement, which may in turn result in increased membrane rigidity and decreased cellular
deformability. 相似文献