Three-dimensional tracking of GLUT4 vesicles in TIRF microscopy

TIRF microscopy has provided a means to view mobile granules within 100 nm in size in two dimensions. However quantitative analysis of the position and motion of those granules requires an appropriate tracking method. In this paper, we present a new tracking algorithm combined with the unique features of TIRF. Firstly a fluorescence correction procedure was processed to solve the problem of fluorescence bleaching over time. Mobile granules were then segmented from a time-lapse image stack by an adaptive background subtraction method. Kalman filter was introduced to estimate and track the granules that allowed reducing searching range and hence greater reliability in tracking process. After the tracked granules were located in x-y plane, the z-position was indirectly inferred from the changes in their intensities. In the experiments the algorithm was applied in tracking GLUT4 vesicles in living adipose cells. The results indicate that the algorithm has achieved robust estimation and tracking of the vesicles in three dimensions.     

From research to reality: Australian evidence-based practice in autism education

Many teachers are sporadic in using evidence-based practice (EBP) concerning students with autism spectrum disorder (ASD). Numerous reasons have been posited for this. However, no single study has worked to understand the relative importance of identified criteria in the EBP decision-making processes of teachers. Through the development of a new survey tool, the Evidence-Based Practice Innovation Survey (EBPIAS), an exploration of the decision-making process of Australian teachers in their adoption and cessation of EBP was undertaken. Teachers rated the perception that an EBP would meet the needs of their students as the most important criteria in determining their use of EBP. Conversely, the judgement that an EBP did not meet the needs of their student was the most important criteria in their decision to cease use. In comparing the relevant importance of identified criteria, teachers rated the perception of training as the second-lowest consideration in their decision to adopt and cease using EBP. Consequently, there may need to be greater promotion of, or information concerning, the training required by teachers for effective implementation supporting a more balanced and holistic approach in assessing criteria impacting the adoption of EBP by teachers in the first instance, potentially lowering cessation rates.     

视频示范教学法在自闭症谱系障碍者职业技能训练中的应用

视频示范教学法在自闭症谱系障碍者职业技能训练中的应用模式包括基本视频示范教学模式、自我视频示范模式、视频提示和视频反馈,并经常与虚拟现实和提示系统等其他循证实践一起使用。未来的研究和实践中应使用视频示范教学加强对非操作性技能的训练,探索更具竞争性的职业种类进行训练,培训专业人员,研发视频示范教学关联软件、注重被试样本的多样性及差异性、比较不同应用模式的干预效果。     

地美硝唑与牛血清白蛋白结合热力学特征的荧光光谱研究

采用荧光光谱和紫外可见吸收光谱法研究了地美硝唑与牛血清蛋白的结合反应,发现地美硝唑对牛血清白蛋白有较强的荧光猝灭作用,该猝灭过程主要为静态猝灭过程。从荧光光谱数据,由Stern—Volmer方程和Lineweaver—Burk方程分析并处理实验数据得到了23℃时结合反应的结合常数K=1．03×10^4L／mol,结合位点数为0．70,结合反应的标准焓变、标准熵变、和标准吉布斯自由能变分别为～63．48kJ／mol,-96．22J／K,-35．00kJ／mol。     

2-氨基-6-甲基-3-乙氧羰基-5,7-二苯基-4,5,6,7-四氢吡啶并噻吩的合成与结构表征

首先利用醛酮缩合制得二烯酮（1）,然后利用1与甲胺溶液作用制得哌啶酮（2）,最后利用Gewald反应使2、氰乙酸乙酯与硫磺在吗啡啉催化下,得到未见文献报道的2-氨基四氢吡啶并噻吩衍生物3,产率60%～80%。所得产物的结构由元素分析,IR,1H NMR和MS所确证。     

Er^3＋：Y0.5Gd0.5VO4晶体生长和缺陷研究

采用液相法合成了多晶材料,用高温提拉法（CZ法）生长出Er^3＋：Y0.5Gd0.5VO4晶体。用ICP光谱法测定晶体中Er^3＋离子含量为1．25at％,分凝系数为1．06。采用热磷酸腐蚀晶片,在偏光显微镜下对晶体的（010）、（001）面上的缺陷进行了观察,可明显观察到位错线、生长层和包裹物。南此得出,Er^3＋：Y0.5Gd0.5VO4晶体的主要缺陷是位锚、生长层和包裹物等。     

Beyond Interests, Ideas, and Technology: An Institutional Approach to Communication and Information Policy

This article examines the theoretical assumptions generally used in communication and information policy studies, and suggests that more attention to the institutional determinants of public policies is needed. The first part discusses three alternative theoretical approaches: the interest-group approach, the ideological approach, and the technology-centered approach. The second part outlines the conceptual tools of the new institutionalism approach and discusses its application for the study of regime change in telecoms and media. The third part presents an abbreviated example of such application to the case of U.S. spectrum policies and the licensing of digital broadcasting. The conclusion suggests new directions for research aimed at broadening the set of social actors participating in the global governance of new technologies.     

含卤素基团振动波数关系

本文从大量数据中总结出:组成及结构类似的含卤素基团的振动波数基本上按F、Cl、Br、I的顺序降低     

数学中三种新的数量形式

分析了传统无穷理论体系及与之相关的数学内容中的缺陷，提出数学中三种新的数量形式，并构造了一个与新无穷观相对应的新数谱。     

多元智能理论与学前儿童音乐教育——美国多彩光谱项目及其对我国学前儿童音乐教育的启示

本文对美国以多元智能理论为基础的多彩光谱项目作了简要的介绍，分析了多彩光谱项目中的音乐领域与我国学前音乐教育相比较之新特点，以及该项目对我国学前儿童音乐教育改革的启示。