大菜粉蝶颗粒体病毒(pbGV)包涵体蛋白mRNA的提取及电镜观察

包涵体蛋白 mRNA 是大菜粉蝶颗粒体病毒(pbGV)感染晚期合成的主要信使 RNA.我们从染病晚期幼虫脂肪体中,用饱和苯酚法提取总核酸,用 LiCl 去除其中 DNA,经 Oligo-(dT)纤维素层析柱,mRNA 呈现二个洗脱峰,将洗脱峰组份用尿素-高甲酰胺法制样电镜观察.电镜下 mRNA 分子柔软、粗糙,呈弯曲的线状,分子长度约0.3μ(30个统计)     

大麦胚性愈伤组织细胞ODO冷冻断裂面的扫描电镜观察

ODO冷冻断裂法是一项扫描电镜生物样品制备的有效技术,它在生物医学的形态学研究中发挥了重要作用,把这个技术用到植物离体培养物的超微形态研究,是一个新的尝试.大麦胚性愈伤组织用低浓度的戊二醛前固定,再用ODO冷冻断裂法制备扫描电镜样品.本研究主要观察了淀粉粒和淀粉质体在胚性愈伤组织细胞中的分布,因为淀粉粒沉积的变化和质体结构的变化是去分化和再分化过程中的重要特征之一.本文还图示了自制的冷冻断裂器,并对ODO冷冻断裂法的应用作了一些讨论.     

Design Principles for Computer-assisted Instruction in Histology Education: An Exploratory Study

The purpose of this paper is to describe the development process and the key components of a computer-assisted histology material. Computer-assisted histology material is designed to supplement traditional histology education in a large Midwestern university. Usability information of the computer-assisted instruction (CAI) material was obtained through formative research methodology. Findings indicate that computer-assisted instruction should be used as complimentary to traditional histology instruction.     

自旋极化输运的方法讨论

电子自旋注入和自旋相关输运是自旋电子学中被广泛研究的课题。本文将介绍在自旋电子学器件上具有广泛应用前景的不同结构中物理原理相关的材料问题,如F/I/S隧穿、F/I/F隧穿、Andreev反射。     

反对称磁垒纳米结构中的电子自旋极化效应

提出了一种反对称磁垒纳米结构,并研究了其电子自旋输运的性质。结果表明,当考虑电子自旋与非均匀磁场的相互作用时,这种磁垒纳米结构具有很强的电子自旋极化效应。而且,电子的自旋极化度与系统的结构参数密切相关,因此通过调整磁条可以调控系统中自旋极化电子的行为。     

量子点的输运特性研究

研究了电子隧穿通过量子点的相干输运特性。考虑量子点上的库仑相互作用,得到了电导—门压的共振隧穿曲线,并讨论其物理意义。     

Seeking Nettle Textiles – Utilizing a Combination of Microscopic Methods for Fibre Identification

Bast fibres have been commonly used as a textile material in Northern Europe since Neolithic times. However, the process of identifying the different species has been problematic, and many important questions related to their cultural history are still unanswered. For example, a modified Herzog test and the presence of calcium oxalate crystals have both been used in identification. In order to generate more reliable results, further research and advancement in multi-methodological methods is required. This paper introduces a combination of methods which can be used to identify and distinguish flax (Linum usitatissimum), hemp (Cannabis sativa), and stinging nettle (Urtica dioica). The research material consisted of reference fibres and 25 fibre samples obtained from 12 textiles assumed to be made of nettle. The textiles were from the Finno-Ugric and Historical Collections of The National Museum of Finland. The fibre samples were studied by observing the surface characteristics and cross sections with transmitted light microscopy, and by using a modified Herzog test with polarized light, in order to identify the distinguishable features in their morphological structures. The study showed that five out of 25 samples were cotton, 16 nettle, one flax, and one hemp. Findings from two samples were inconsistent. The results show that it is possible to distinguish common north European bast fibres from each other by using a combination of microscopic methods. Furthermore, by utilizing these combined methods, new and more reliable information could be obtained from historical ethnographic textiles, which creates new vistas for the interpretation of their cultural history.     

钻孔法替代巷道法进行采空区瓦斯治理的实践

顶板走向长钻孔替代瓦斯抽采巷道抽采采空区顶板裂隙带瓦斯技术,是淮南矿区研发的瓦斯治理新技术,该技术在丁集煤矿的1262（1）工作面进行实践,取得了预期效果;根据1262（1）工作面的地质条件,设计合理的顶板走向抽采钻孔,通过对获取的抽采数据分析,得出了在采煤工作面顺层钻孔预抽后瓦斯含量小于10m^3／t时,可以用钻孔法代替巷道法抽采瓦斯。     

大学英语泛读教学浅析

就大学英语当中应注重学生泛读的培养谈了一些观点。阅读也是今后有效交际的重要手段之一，本文就其作用及如何能够提高泛读的水平与阅读速度做了一些较为详尽的阐述。掌握泛读的技巧对于学生今后有效获取信息知识，与有效的进行交流有看至关重要的作用。     

An evaluation of outcomes following the replacement of traditional histology laboratories with self‐study modules

Changes in medical school curricula often require educators to develop teaching strategies that decrease contact hours while maintaining effective pedagogical methods. When faced with this challenge, faculty at the University of Cincinnati College of Medicine converted the majority of in‐person histology laboratory sessions to self‐study modules that utilize multiple audiovisual modalities and a virtual microscope platform. Outcomes related to this shift were investigated through performance on in‐house examinations, results of the United States Medical Licensing Examination^® (USMLE^®) Step 1 Examination, and student feedback. Medical School College Admissions Test^® (MCAT^®) scores were used as a covariate when comparing in‐house examinations. Results revealed no significant change in performance on in‐house examinations when the content being assessed was controlled (F(2, 506) = 0.676, P = 0.51). A significant improvement in overall practical examination grade averages was associated with the self‐study modules (F(6, 1164) = 10.213, P < 0.01), but gradual changes in examination content may explain this finding. The histology and cell biology portion of USMLE Step 1 Examination remained consistent throughout the time period that was investigated. Student feedback regarding the self‐study modules was positive and suggested that features such as instructor narrated videos were an important component of the self‐study modules because they helped recreate the experience of in‐person laboratory sessions. Positive outcomes from the student perspective and no drop in examination performance suggests that utilizing self‐study modules for histology laboratory content may be an option for educators faced with the challenge of reducing contact hours without eliminating content. Anat Sci Educ 10: 276–285. © 2016 American Association of Anatomists.